1st Edition

Basics of CRISPR/Cas Mediated Plant Genome Editing

Edited By Anshu Alok, Jitesh Kumar, Mahipal Shekhawat Copyright 2025
256 Pages 17 Color Illustrations
by CRC Press

256 Pages 17 Color Illustrations
by CRC Press

256 Pages 17 Color Illustrations
by CRC Press

CRISPR/Cas has emerged, as a powerful genetic engineering tool. It is simple yet highly affordable and robust. This book provides a basic introduction to the mechanism and architecture of the CRISPR/Cas. It enlightens the readers about the new advancements such as gene insertion, transcriptional activation, suppression, gene tagging, multiplexing, base editing, prime editing, and tissue culture... Read more

Preface

Introduction to Genome Editing Tool in Plants  

Navjot Kaur, Zienab Ahmed, Anshu Alok and Jitesh Kumar

The Mechanism of CRISPR and Its Classification

Alka Jangra, Garima Sharma, Siddhant Chaturvedi and Vinod Chhokar

Classification of the CRISPR/Cas System

Mitrabinda Panda, Seema Pradhan and Late. Ajay Parida

Emergence of CRISPR Technology Bringing Revolution in Plant Biotechnology

Nandita Thakur, Phanikanth Jogam and Baohong Zhang

CRISPR/Cas Delivery Methods in Plants

Neetu Singh Kushwah, Shristi Verma, Meenal Rathore and NP Singh

Bioinformatics Approaches to Guide RNA Designing and Off-target Prediction

Vinita Sharma, Himanshu Sharma, Chibuikem Unamba, Joy Roy and Abhishek Bhandawat

Importance of Tissue Culture in Genome Editing

Bushra Hafeez Kiani

Multiplex Genome Editing Approaches for the Plant Genetic Manipulations

Sukhmandeep Kaur, Sanskriti Vats, Bharti Aggarwal, Jashandeep Kaur and Anupriya Bhardwaj

Transcriptional Activation and Suppression using CRISPR/Cas9: A Plant’s Outlook

Ayyagari Ramlal, Hardik Singhal, Kamal Kumar, Lalit Kumar, Vimal Pandey and Sahil Mehta

CRISPR/Cas-Based Genome Editing for Soybean Improvement

Ashita Bisht, Dinesh Kumar Saini, Pankaj Kumar and B S Gill

Application of CRISPR Technology in Plant Salt Tolerance and Membrane Proteins

Debajyoti Dutta and Prabir Kumar Das

Index

Biography

Dr. Anshu Alok has expertise in plant genetic engineering and genome editing. He has used genome editing in tobacco, potato, wheat, and banana. He has authored over 50 peer-reviewed research and review articles, and book chapters. Dr. Alok has served as editor in 3 Biotech, IJMS and still serves on the Editorial Boards of BMC Plant Method and Frontier in Genome editing.

Dr. Jitesh Kumar has expertise in plant biology, particularly genome editing. He has authored over 25 peer-reviewed papers and book chapters. Dr. Kumar actively contributes to the academic community by serving on the Editorial Boards of BMC Plant Biology and Biotechnology.

Dr. Mahipal S. Shekhawat is an expert in the area of plant cell and tissue culture. He is working on the micropropagation of medicinal & horticultural crops, plant structural & behavioral responses, and biosynthesis of nanoparticles and their applications in crop improvement. He has authored over 150 peer-reviewed research papers.