1st Edition

Theory and Practice

ISBN 9780367455033
Published February 14, 2002 by CRC Press
544 Pages

USD $69.95

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Book Description

The field of bioseparation, and biochromatography in particular, is advancing very rapidly as our knowledge of the properties of molecules and atomic forces increases. This volume covers the basic principles of biochromatography in detail. It assesses different techniques and includes a large number of applications, providing the reader with a multidisciplinary perspective that gives the insight to master the many chromatographic methods. Biochromatography: Theory and Practice is a valuable tool for graduate and research scientists, technicians, engineers and teachers in a range of fields including biochemistry, biotechnology, biorecognition and chromatography.

Table of Contents

Upstream and Downstream Steps in Biochemistry. Gel Filtration. Ion Exchange Interaction Chromatography. Hydrophobic ( Interaction ) Chromatography of Proteins. Conformational Behaviour of Polypeptides and Proteins in Reversed Phase and Lipophilic Environments. Affinity Chromatography. Dye Ligand Affinity Chromatography. Immobilized Synthetic Dyes in Affinity Chromatography. Immobilized Histidine Ligand Affinity Chromatography. Immobilized Metal-Ion Affinity Chromatography: From Phenomenological Hallmarks to Structure-Based Molecular Insights. Thiophilic Interaction Chromatography. Miscellaneous Methods in Affinity Chromatography Part 1: Boronic Acids as Selective Ligands for Affinity Chromatography. Part 2: Shielded Affinity Chromatography in Packed Bed and Expanded Bed Mode. Glycobiology and Biochromatography. Capillary Electrokinetic Chromatography. Imprintec Polymers as Tailor-Made Stationary Phases for Affinity Separation. Computer-aided Simulation of Biochromatography. Industrial Biochromatography: Engineering Aspects. Validation Aspects in Biochromatography. Biochromatography and Biomedical Applications.

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Vijayalakshmi\, M. A.