Biofilms: Investigative Methods and Applications, 1st Edition (Hardback) book cover

Biofilms

Investigative Methods and Applications, 1st Edition

By Hans-Curt Flemming, Ulrich Szewzyk, Thomas Griebe

CRC Press

268 pages

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Hardback: 9781566768696
pub: 2000-06-23
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Description

Until now, techniques for studying biofilms- the cellular colonies that live in drinking water systems, wastewater operations, even ground and surface water- have been limited. Yet during the last decade, biofilms have become a critical element in water quality preservation systems, a key component of wastewater treatment biological reactions and the subject of extensive microbiological inquiry. An understanding of biofilm development, structure and dynamics is one condition for improving water supplies and for addressing technical problems such as biofouling, corrosion and bioweathering.

Biofilms: Investigative Methods and Applications provides the first in-depth assessment of current and experimental ways to study biofilms, both in sample form and in situ. It shows how sensors, microscopy, lasers, and calorimetry, among other tools, can be used to obtain data on the morphology and metabolism of biofilms. This text is the first to organize and examine the best methods for investigating biofilms. It covers culture-based methods and emerging nondestructive techniques. It also shows how they can be used to characterize biofilms in a variety of manmade settings, such as sewers, wastewater plants, and drinking water distribution systems, as well as in karsts and groundwater sources.

In clarifying the way biofilms are studied, the book offers new insights into these living films. It also applies inquiry techniques to the many problems confronting the environmental specialist-corrosion control, biofouling, and the improvement of fixed reactors in wastewater treatment. At the same time it explains technologies for the controlled growth of biofilms and shows how biofilms can be effectively monitored and subjected to quantitative analysis. The technical information in Biofilms: Investigative Methods and Applications is designed to be of use to engineers and researchers and to be helpful in the generation of electronic data.

Table of Contents

Preface

List of Contributors

Steps in Biofilm Sampling and Characterization in Biofouling Cases by Gabriela Schaule, Thomas Griebe and Hans-Curt Flemming

  • Introduction

  • Sampling Strategy

  • Field Methods In Situ

  • Laboratory Methods

  • Laboratory Methods to Examine Removed Biofilms

  • Identification

  • Chemical and Biochemical Analysis

  • Genetic Methods

  • Immunological Methods

  • Conclusions and Outlook

  • References

    Rotating Annular Reactors for Controlled Growth of Biofilms by Thomas Griebe and Hans-Curt Flemming

  • Introduction

  • Materials and Methods

  • Results

  • Discussion

  • References

    Microcalorimetry for Evaluating Countermeasures against Biofouling in Water Circulation Systems by Henry Von Rége and Wolfgang Sand

  • Introduction

  • Materials and Methods

  • Results and Discussion

  • Conclusions

  • Acknowledgement

  • References

    Extraction and Quantification of Extracellular Polymeric Substances from Wastewater by Rosemarie Spaeth and Stefan Wuertz

  • Introduction

  • Extraction Methods

  • Quantification

  • Cell Lysis

  • Applications

  • Acknowledgements

  • Nomenclature

  • References

    Cell Biomass Determination in Sewer Biofilms and a Monospecies Pseudomonas putida Biofilm by Andreas Jahn and Per Halkjaer Nielsen

  • Introduction

  • Direct Determination

  • Indirect Methods

  • Conclusions

  • References

    Simultaneous Morphological and Population Analyses of Environmental Biofilms by Jannie A. Flood, Nicholas J. Ashbolt and Peter J. Beatson

  • Principle of the Method

  • Range of Applications

  • Methods

  • Results

  • Comparison with Other Methods

  • Discussion

  • Nomenclature

  • Acknowledgements

  • References

    Development of a New Method to Determine the Metabolic Potential of Bacteria in Drinking Water Biofilms: Probe Active Counts (PAC) by Sibylle Kalmbach, Werner Manz and Ulrich Szewzyk

  • Introduction

  • Materials and Methods

  • Results and Discussion

  • References

    Formation and Bacterial Composition of Young, Natural Biofilms Obtained from Public Bank-Filtered Drinking Water Systems by Thomas Schwartz, Sandra Hoffmann and Ursula Obst

  • Introduction

  • Materials and Methods

  • Results

  • Discussion

  • Acknowledgements

  • References

    The Use of Immunological Techniques and Scanning Confocal Laser Microscopy for the Characterization of Agrobacterium tumefaciens and Pseudomonas fluorescens, Atrazine-Utilizing Biofilms by Martina Hausner, John R. Lawrence, Gideon M. Wolfaardt, Michael Schloter, Klaus-Peter Seiler and A. Hartmann

  • Introduction

  • Materials and Methods

  • Results

  • Discussion

  • Acknowledgements

  • References

    The in situ Detection of a Microbial Biofilm Community on Karst Rock Coupon in a Groundwater Habitat by E. Müller, B. Assmus, A. Hartmann and K.-P. Seiler

  • Introduction

  • Materials and Methods

  • Results and Discussion

  • Summary

  • References

    Investigation of Spatial and Temporal Gradients in Fixed-Bed Biofilm Reactors for Wastewater Treatment by Axel Wobus, Kerstin Röske and Isolde Röske

  • Introduction

  • Aim of the Study

  • Experimental Setup

  • Chlorophenol Elimination by Biofilms under Different Operating Conditions

  • Quantitative Determination of Biomass and Biological Activity

  • Investigation of the Biomass and Biological Activity

  • Investigation of the Bacterial Community of the Biofilm

  • The Protozoa and Metazoa in Biofilms

  • Conclusions

  • Nomenclature

  • Acknowledgements

  • References

    Quantitative Microscopy in Biofilm Studies by Martin Kuehn, Ulrich Schindler, Peter A. Wilderer and Stefan Wuertz

  • Introduction

  • Materials and Methods

  • Results

  • Discussion

  • Acknowledgements

  • References

    Confocal Laser Scanning Microscopy (CLSM) of Biofilms by Thomas R. Neu

  • Introduction

  • CLSM History

  • Confocal Imaging

  • Advantages/Disadvantages

  • Techniques

  • Applications

  • Conclusions

  • Acknowledgement

  • References

    Microelectrodes and Tube Reactors in Biofilm Research by Harald Horn

  • Introduction

  • Materials and Methods

  • Results

  • Discussion

  • References

    Index

  • Subject Categories

    BISAC Subject Codes/Headings:
    TEC010030
    TECHNOLOGY & ENGINEERING / Environmental / Water Supply