Biotechnology Fundamentals: 2nd Edition (Paperback) book cover

Biotechnology Fundamentals

2nd Edition

By Firdos Alam Khan

CRC Press

Purchasing Options:$ = USD
New in Paperback: 9780815370048
pub: 2017-09-07
$67.95
x
Hardback: 9781498723428
pub: 2015-11-23
$175.00
x
eBook (VitalSource) : 9781315370767
pub: 2018-09-03
from $32.48


FREE Standard Shipping!

Description

A single source reference covering every aspect of biotechnology, Biotechnology Fundamentals, Second Edition breaks down the basic fundamentals of this discipline, and highlights both conventional and modern approaches unique to the industry. In addition to recent advances and updates relevant to the first edition, the revised work also covers ethics in biotechnology and discusses career possibilities in this growing field.

The book begins with a basic introduction of biotechnology, moves on to more complex topics, and provides relevant examples along the way. Each chapter begins with a brief summary, is illustrated by simple line diagrams, pictures, and tables, and ends with a question session, an assignment, and field trip information. The author also discusses the connection between plant breeding, cheese making, in vitro fertilization, alcohol fermentation, and biotechnology.

Comprised of 15 chapters, this seminal work offers in-depth coverage of topics that include:

  • Genes and Genomics
  • Proteins and Proteomics
  • Recombinant DNA Technology
  • Microbial Biotechnology
  • Agricultural Biotechnology
  • Animal Biotechnology
  • Environmental Biotechnology
  • Medical Biotechnology
  • Nanobiotechnology
  • Product Development in Biotechnology
  • Industrial Biotechnology
  • Ethics in Biotechnology
  • Careers in Biotechnology
  • Laboratory Tutorials

Biotechnology Fundamentals, Second Edition provides a complete introduction of biotechnology to students taking biotechnology or life science courses and offers a detailed overview of the fundamentals to anyone in need of comprehensive information on the subject.

Reviews

"Dr. Khan’s Biotechnology Fundamentals serves as a great introduction to the field; presenting the various topics with clear definitions along with real-world examples and applications. In this second edition, Dr. Khan has included an introduction to several new exciting interdisciplinary areas such as biorobotics, biomimetics and epigenetics. These are all relatively new areas in biotechnology that show great promise and are exciting areas to explore."

—Alexandra Gorgevska, Palm Beach State College, Lake Worth, Florida, USA

Table of Contents

Introduction to Biotechnology

Learning Objectives

What Is Biotechnology?

Animal Biotechnology

Agricultural Biotechnology

Medical Biotechnology

Industrial Biotechnology

Environmental Biotechnology

Other Emerging Fields of Biotechnology

History of Biotechnology

Human Genome Project

Major Scientific Discoveries in Biotechnology

Biotechnology as the Science of Integration

Bio-Revolution

Ethical and Regulatory Issues in Biotechnology

Future of Biotechnology

Problems

Assignment

Online Resources

References and Further Reading

Genes and Genomics

Learning Objectives

Introduction

Cell as the Building Block of Life

Classification of Cells

Prokaryotic Cell

Eukaryotic Cell

Extracellular Organization

Cell Membrane

Cell Capsule

Flagella

Intracellular Organization

Macromolecules

Genes and Genetics

Cell Division

DNA Replication

DNA Interactions with Proteins

DNA-Modifying Enzymes

DNA Methylation

DNA Mutation

Tools of Biotechnology

Problems

Assignment

References and Further Reading

Proteins and Proteomics

Learning Objectives

Introduction

Significance of Proteins

Protein Biosynthesis

Protein Structure

Protein Folding

Protein Modification

Protein Transport

Protein Dysfunction and Degradation

Regulation of Protein Synthesis

Regulatory Protein

Methods for Protein Analysis

Protein Purification

Tools of Proteomics

Problems

Assignment

References and Further Reading

Recombinant DNA Technology

Learning Objectives

Introduction

Making of Recombinant DNA

Significance of Recombinant DNA Technology

Role of Restriction Enzymes in rDNA Technology

Steps in Gene Cloning

Synthesis of Complete Gene

Polymerase Chain Reaction and Gene Cloning

Significance of Vectors in Recombinant DNA Technology

Classification of Vectors

Integration of the DNA Insert into the Vector

Introduction of the Recombinant DNA into the Suitable Host

Increased Competence of E. Coli by CaCl2 Treatment

Infection by Recombinant DNAs Packaged as Virions

Selection of Recombinant Clones

Identification of Clones Having Recombinant DNAs

Selection of Clone Containing a Specific DNA Insert

Applications of Recombinant DNA Technology

DNA Sequencing

Microarrays

DNA Chips

Isolation of Desired DNA

cDNA Library

Preparation of cDNA

Genomic Library

DNA Libraries

Chemical Synthesis Gene

Applications of Synthetic Oligonucleotides

Problems

Assignments

References and Further Reading

Microbial Biotechnology

Learning Objectives

Introduction

Structural Organization of Microbes

Microbial Metabolism

Microbial Growth

Microbial Genetics

Genetic Recombination in Bacteria

Transposable Genetic Elements

Use of E. Coli in Microbial Cloning

Pathogenic Bacteria

Application of Microbes

Food Microbiology

Microbial Biotechnology

Problems

References and Further Reading

Agricultural Biotechnology

Learning Objectives

Introduction

Plant Breeding

Plant Diseases

Applications of Molecular and Genetic Tools in Agriculture

Herbicide-Tolerant Plants

Pigmentation in Transgenic Plants

Altering the Food Content of Plants

Gene Transfer Methods in Plants

Target Cells for Gene Transformation

Vectors for Gene Transfer

Transformation Techniques Using Agrobacterium

Glucuronidase Gene

Agroinfection and Gene Transfer

DNA-Mediated Gene Transfer

Electroporation for Gene Transfer

Liposome-Mediated Gene Transfer

Gene Transformation Using Pollen

Application of Transgenic Plants

How Safe Are Transgenic Plants?

Bioengineered Plants

Genetically Modified Maize

Terminator Technology

Problems

Assignment

References and Further Reading

Animal Biotechnology

Learning Objectives

Introduction

History of the Use of Animals in Research

Drug Testing in Animals Is Mandatory

Most Commonly Used Animals in Research

Application of Animal Models

Animal Models

Animal Biotechnology

Regulations of Animal Testing

Alternatives to Animal Testing

Problems

Assignment

References and Further Reading

Environmental Biotechnology

Learning Objectives

Introduction

Factors Affecting the Environment

Global Warming

Carbon Footprint

Destruction of Forests

Air Pollution

Major Carbon Dioxide Emission Countries

Greenhouse Effect

Acid Rain

Ocean Acidification

Health Hazards Due to Pollution

Environment Protection by Biotechnology

Bioremediation

Mycoremediation

Wastewater Treatment

Pretreatment Phase

Screening Phase

Sedimentation Phase

Secondary Treatment Phase

Activated Sludge

Filter Beds

Biological Aerated Filters

Nutrient Removal

Nitrogen Removal

Phosphorus Removal

Disinfection of Wastewater

Sludge Disposal

Biofuels

Biodiesel

Biogas

Bioalcohols

Bioethers

Syngas

Solid Biofuels

Second-Generation Biofuels

Third-Generation Biofuels

International Biofuel Efforts

Future of Biofuels

Biodegradable Plastic

Biodegradation by Bacteria

Oil-Eating Bacteria

Biostimulation and Bioaugmentation

Bioleaching

Single-Cell Protein and Biomass from Waste

Vermitechnology

Biosorption

Bacteria

Fungi

Algae

Genetically Engineered Organisms

Problems

Debate

Field Visit

References and Further Reading

Medical Biotechnology

Learning Objectives

Introduction

Antibody Production

Therapeutic Proteins

Stem Cell Transplantation

Bioengineered Skin

Bioengineered Organ Transplantation

Gene Therapy

Molecular Diagnostics

Artificial Blood

Organ Transplant

Cloning

Questions

Assignment

References and Further Reading

Nanobiotechnology

Learning Objectives

Introduction

Nanotechnology

Nanobiotechnology

Applications of Nanobiotechnology

Nanotechnology in the Food Industry

Water Pollution and Nanotechnology

Research Trends

Problems

References and Further Reading

Product Development in Biotechnology

Learning Objectives

Introduction

Methods of Scientific Inquiry

Commercialization of Scientific Discovery

Business Plan

Biotechnology Product Development

Phases of Biotechnology Product Development

Biotechnology Entrepreneurship

Biotechnology Industry: Facts and Figures

Formation of a New Biotechnology Company

Successful Bioentrepreneurship

Biotechnology Products and Intellectual Property Rights

Biotechnology Stock Investment: Pros and Cons

Marketing Trends in Biotechnology

Role of Regulators in Biotechnology Product Development

Certification and Accreditation

Problems

References and Further Reading

Industrial Biotechnology

Learning Objectives

Introduction

Fermenter or Bioreactor

Principle of Fermentation

Production of Biomolecules Using Fermenter Technology

Development Process of Microbial Products

Upstream Bioprocess

Downstream Bioprocess

Bioprocess Automation

Industrial Application of Microbes

Industrial Production of Healthcare Products

Problems

References and Further Reading

Ethics in Biotechnology

Learning Objectives

Introduction

Genetically Modified Foods and Plants

Use of Animals as Experimental Models

Use of Humans as Experimental Models

Xenotransplantation

Genetic Screening

Biometrics

DNA Fingerprinting

Organ Donation and Transplantation

Euthanasia

Neuroethics

Assisted Reproductive Technology

Embryonic Stem Cell Research

Questions

Assignments

References and Further Reading

Careers in Biotechnology

Learning Objectives

Introduction

Education and Investment in Biotechnology

Research and Development in Biotechnology

Biotechnology Industry and Products

Biotechnology Status in the United States

Career Opportunities in Biotechnology

Entry-Level Job Positions in Biotechnology

Administration

Which Job Is Good For Me?

Why Are R&D Jobs the Most Challenging?

Salary and Incentives in Biotechnology

Problems

Assignments

Field Visit

References and Further Reading

Laboratory Tutorials

Laboratory Experiments

Laboratory Safety

Good Laboratory Practices for Biotechnology Labs

General Laboratory Techniques

General Principles of Animal Handling

Animal Anesthesia

Animal Histology

Blood Collection in Animals

Histology and Microscopy

Separation Techniques

Microbiology Techniques

Biochemistry Techniques

Molecular Techniques

Genetic Techniques

Agricultural Biotechnology

Microbial Biotechnology

References

About the Author

Firdos Alam Khan is a professor and chairperson of the School of Life Sciences, Manipal University, Dubai, United Arab Emirates (UAE). He earned his PhD from Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra, India, in 1997. He has more than 21 years of research and teaching experience in various domains of biotechnology. His area of expertise includes stem cell technology and neuroscience. He has written numerous articles in various national and international journals in the areas of neuroscience, neuropharmcology, and stem cell biology, and has been an editorial board member of the EC Microbiology and MOJ Immunology scientific journals.

Subject Categories

BISAC Subject Codes/Headings:
MED009000
MEDICAL / Biotechnology
SCI010000
SCIENCE / Biotechnology
SCI043000
SCIENCE / Research & Methodology
TEC059000
TECHNOLOGY & ENGINEERING / Biomedical