1st Edition

Biotechnology and Crop Improvement Tissue Culture and Transgenic Approaches

Edited By Nitish Kumar Copyright 2023
    268 Pages 10 Color & 2 B/W Illustrations
    by CRC Press

    268 Pages 10 Color & 2 B/W Illustrations
    by CRC Press

    Biotechnology and Crop Improvement

    The green revolution led to the development of improved varieties of crops, especially cereals, and since then, classical or molecular breeding has resulted in the creation of economically valuable species. Thanks to recent developments in biotechnology, it has become possible to introduce genes from different sources, such as bacteria, fungi, viruses, mice and humans, to plants. This technology has made the scientific community aware of the critical role of transgenic, not only as a means of producing stress tolerant crops but also as a platform for the production of therapeutics through molecular farming. Biotechnology and Crop Improvement: Tissue Culture and Transgenic Approaches focuses on important field crops to highlight germplasm enhancement for developing resistance to newly emerging diseases, pests, nutrient- and water-use efficiency, root traits and improved tolerance to increasing temperature and introduces significant recent achievements in crop improvement using methods such as micropropagation, somaclonal variation, somatic embryogenesis, anther/pollen/embryo culture, and compressing the breeding cycle for accelerated breeding and early release of crop varieties.

    Plant biotechnology has now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenic now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. Development of transgenic crop plants, their utilization for improved agriculture, health, ecology and environment and their socio-political impacts are currently important fields in education, research, and industry and also of interest to policy makers, social activists and regulatory and funding agencies. This work prepared with a class-room approach on this multidisciplinary subject will fill an existing gap and meet the requirements of such a broad section of readers. It describes the recent biotechnological advancement and developments in plant tissue culture and transgenic. Plant tissue culture techniques such as such as micropropagation, regeneration, somaclonal variation, somatic embryogenesis, anther/pollen/embryo culture are discussed for genetic improvement of crop plant. Transgenic techniques are discussed for developing resistance to newly emerging diseases, pests, nutrient- and water-use efficiency, root traits, and improved tolerance to increasing temperature.

    Key Features

    • Shows the importance of plant tissue culture and transgenic technology on plant biology research and its application to agricultural production
    • Provides insight into what may lie ahead in this rapidly expanding area of plant research and development
    • Contains contributions from major leaders in the field of plant tissue culture and transgenic technology

    This book is devoted to topics with references at both graduate and postgraduate levels. The book traces the roots of plant biotechnology from the basic sciences to current applications in the biological and agricultural sciences, industry, and medicine. The processes and methods used to genetically engineer plants for agricultural, environmental, and industrial purposes along with bioethical and biosafety issues of the technology are vividly described in the book.

    Chapter 1: Transgenic Technology in Crop Improvement
    Anugraha A C, Toji Thomas and Dennis Thomas T

    Chapter 2: Elicitation: A Biotechnological Approach for Enhancement of Secondary Metabolites in in-vitro Cultures
    Ritu Mahajan, Tania Sagar, Pallavi Billowria, Nisha Kapoor

    Chapter 3: Tissue Culture of Rare and Endangered Forest Plant Species of India
    R. S. Sharma, Vikram Singh Gaur, Varsha Kumari, S.R. Maloo

    Chapter 4: Enhancement of Nutritional, Pharmaceutical and Industrial Value of Crops through Genetic Modification with Carotenoid Pathway Genes
    Amar A. Sakure

    Chapter 5: Factors Influencing Somatic Embryogenesis and Regeneration with Particular Reference to Carica Papaya L.
    Manish Shukla, Mala Trivedi & Rajesh K. Tiwari

    Chapter 6: Application of Plant Tissue Culture for Improvement of Centella Asiatica
    Shweta Kumari, Nitish Kumar and Maheshwar Prasad Trivedi

    Chapter 7: Improvement of Seed Protein Quality in some important Food Crops using Genetic Engineering Approaches
    Jitendra Kumar Sharma, Anita Rani Santal, and Nater Pal Singh

    Chapter 8: Somatic Embryogenesis and Transformation Studies in Ginger
    Valiyaparambath Musfir Mehaboob, Kunnampalli Faizal, Palusamy Raja, Ganesan Thiagu, Kizhakke Modongal Shamsudheen and Abubakker Aslam and Appakan Shajahan

    Chapter 9: Role of Biotechnology in Genetic Improvement of Clitoria Ternatea: A Rare Medicinal Plant
    Ambika Gupta, Nitish Kumar

    Chapter 10: Molecular Clonal Fidelity Assessment of Micropropagated Orchids using DNA Markers
    Paonam Sonia, Nandeibam Apana, Abhijit Dey, Imlitoshi Jamir

    Chapter 11: Tissue Culture Studies in Lamiaceae – A Review
    Deepa AV, Dennis Thomas T

    Chapter 12: Cinnamomum Tamala - A Review of its Traditional Uses, Phytochemistry and Pharmacological Properties and Micropropagation
    Priyanka Chaudhary, Shivika Sharma, Vikas Sharma

    Chapter 13: Quantitative Trait Locus (QTL) Mapping in Crop Improvement
    Sharanabasappa B. Yeri, Varsha Kumari, Radheshyam Sharma and Sumer Singh Punia

    Chapter 14: Progress in Genetic Engineering of Pigeonpea [Cajanus cajan (L.) Millsp.] A Review
    Gourab Ghosh, Jasdeep Chatrath Padaria



    Dr. Nitish Kumar is Senior Assistant Professor at the Department of Biotechnology, Central University of South Bihar, Gaya, Bihar, India. Dr. Kumar completed his doctoral research at the Council of Scientific & Industrial Research–Central Salt & Marine Chemicals Research Institute, Bhavnagar, Gujarat, India. He has published more than 60 research articles and book chapters in leading international and national journals and books. He has a wide area of research experience in the field of genetic improvement of crop plant and has received many awards/fellowships/projects from various organizations, for example, the CSIR, DBT, ICAR and SERB-DST, BRNS-BARC, among others. He is an active reviewer for journals, including Biotechnology Reports, Aquatic Botany, Industrial Crops and Products, PLoS One, Plant Biochemistry and Biotechnology, and 3Biotech to name a few. He also serves as an associate editor of the journal Gene.