This book describes new strategies being used to combat disease agents and invertebrate pests. Outstanding experts from the United States, Belgium, China, Guatemala, Japan, Philippines, Singapore, and Thailand have contributed chapters that cover the latest achievements in genetic engineering, emphasizing the microbial and viral biological control agents that can provide environmentally safe, economical control systems. Topics discussed include genetic engineering of Bacillus thuringiensis and B. sphaericus, the development of insect resistance to microbial biocontrol agents, engineering of baculoviruses and nematodes, bioengineering of plants, plant transformation by particle bombardment, fusion of cultured insect cells, new immunodiagnostic assays and control measures against parasitic human diseases, and genetically engineered microbial agents for malaria control. The book also presents improved mass production procedures of microbial and viral biocontrol agents, as well as regulatory and environmental aspects of genetically engineered biocontrol agents. Biotechnology for Biological Control of Pests and Vectors will provide a valuable reference for researchers and students of biological control, microbiology, virology, and molecular biology.
Section 1: Genetic Engineering of Bacterial Biocontrol Agents 1. Bacillus thuringiensis Insecticidal Crystal Proteins: Gene Structure, Action, and Utilization M.J. Adang 2. Mosquitocidal Toxin Gene of Bacillus thuringiensis Subspecies israelensis L.A. Bulla, Jr., K.C. Raymond, and R.M. Faust 3. The Bacillus sphaericus Toxins and Their Potential for Biotechnological Development C. Berry, J. Hindley, and C. Oei 4. Insect Resistance to Bacillus thuringiensis T.B. Stone, S.R. Sims, S.C. MacIntosh, R.L. Fuchs, and C. Marrone Section 2: Genetic Engineering of Viruses And Nematodes 5. Development of Genetically Enhanced Baculovirus Pesticides H.A. Wood 6. Genetic Engineering of Nematodes for Pest Control G.O. Poinar, Jr. Section 3: Bioengineering of Plants 7. Engineering of Insect Resistance in Plants with Bacillus thuringiensis Genes M. Peferoen and H. Van Mellaert 8. Plant Transformation by Particle Bombardment T.M. Klein and P.Maliga 9. Monoclonal Antibodies for Detection of Rice Viruses- Grassy Stunt, Stripe, Dwarf, Gall Dwarf, and Ragged Stunt P.Q. Cabauatan and H. Hibino Section 4: Insect Cell Fusion 10. Fusion of Cultured Insect Cells J. Mitsuhashi Section 5: Medical Aspects 11. Control of Mosquito Vectors by Genetically Engineered Bacillus thuringiensis and B. sphaericus in the Tropics S. Pantuwatana 12. Biomedical Applications of Monoconal Antibodies (McAb) against Entamoeba histolytica (N. Thammapalerd and S. Thapavanij) 13. Human Onchocerciasis: New Immunodiagnostic Assays and Control Mesaures (R. Luj�and E.W. Cupp) Section 6: Mass Production of Microbial And Viral Biocontrol Agents 14. Mass Production of Bacillus thuringiensis and B. sphaericus for Microbial Control of Insect Pests C.C. Beegle, R.I. Rose, and Y. Ziniu 15. Mass Production of Viral Insecticides S.N. Agathos Section 7: Regulatory and Environmental Aspects 16. Federal Regulation of Biotechnology- Jurisdiction of the U.S. Department of Agriculture S. Shantharam and A.S. Foudin 17. Environmental Impacts of Genetically Engineered Microbial and Viral Biocontrol Agents Anne K. Hollander