Biotechnology of Plasma Proteins: 1st Edition (Hardback) book cover

Biotechnology of Plasma Proteins

1st Edition

By Roger L. Lundblad

CRC Press

460 pages | 20 B/W Illus.

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Hardback: 9781439850268
pub: 2012-08-08
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The fractionation of human blood plasma can be considered to be a mature industry, with the basic technology, alcohol fractionation, dating back at least to the 1940s. Many of the products described in the current work have been approved biologics since the 1950s. The information gathered from the development of plasma proteins has proved vital to the development of recombinant therapeutic proteins.

Discussing the role of plasma proteins in current biotechnology, Biotechnology of Plasma Proteins describes the protein composition of human plasma, the fractionation of plasma to obtain therapeutic proteins, and the analysis of these products. It delineates the path from plasma products to recombinant products, and highlights products from albumin, intravenous immunoglobins, and coagulation. It offers a comprehensive review of current techniques for the analysis of proteins including electrophoresis, chromatography, spectrophotometry, mass spectrometry, and updates not published since 1975.

Key Topics

Protein Composition of Plasma

  • Proteomic methods for plasma protein analysis
  • Plasma protein biomarkers
  • Validation of biomarkers
  • Assays for plasma biomarkers

Methods for the Analysis of Protein Products

  • Assay development and validation
  • Electrophoresis
  • Chromatography
  • Immunoassay
  • Mass spectrometry
  • Raman spectroscopy

Plasma Fractionation: Historical and Modern Methods

  • Development of Cohn alcohol fractionation
  • Industrial methods
  • Development of chromatographic methods

Plasma Protein Products of Therapeutic Value

  • Albumin
  • Intravenous immunoglobulin
  • Coagulation products
  • Growth factors
  • Wound management

Table of Contents

Blood, Plasma, Cells, and Other Biological Fluids

Plasma Fractionation and Plasma Protein Products

Methods for the Analysis of Plasma and Plasma Protein Fractions


Structure of Albumin

In Vitro Chemical Modification of Albumin

Albumin as a Carrier

Ligand-Binding by Albumin

Purification and Characterization of HSA for Clinical Use

Biological Activity of Albumin

Enzymatic Activity of Albumin

Clinical Use of Albumin

Albumin as Diagnostic/Biomarker

Pharmacokinetics of Albumin

Analytical Methods for Albumin Including Use of Albumin as Standard for Analytical Methods

Albumin as Excipient



Plasma Immunoglobulins

Factor VIII and von Willebrand Factor

von Willebrand Factor

Economic Issues Impacting the Hemophilia Business

Conclusions and Future Directions


Plasma Proteinase Inhibitors


α1-Antitrypsin (α1-Antiprotease Inhibitor, SERPINA1)

Heparin Cofactor II (SERPIND1)


Tissue Factor Pathway Inhibitor


C1-Inhibitor (C1-Esterase Inhibitor)

Plasminogen Activator Inhibitor-1

Protein C Inhibitor (Plasminogen Activator Inhibitor-3)


Vitamin K-Dependent Proteins

Miscellaneous Plasma Proteins


Fibrin Sealant

Autologous Fibrin Sealant

Fibrinogen and Tissue Soldering

Thrombin and Fibrin Foam

Fibrinogen Plastics


Plasminogen and Plasmin




About the Series

Protein Science

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biochemistry
MEDICAL / Biotechnology
MEDICAL / Hematology
SCIENCE / Life Sciences / General