An indispensable guide to buffers and to understanding the principles behind their use. Helps the user to avoid common errors in preparing buffers and their solutions.
A must for researchers in the biological sciences, this valuable book takes the time to explain something often taken for granted - buffers used in experiments. It answers the common questions such as: which buffer should I choose? What about the temperature effects? What about ionic strength? Why is the buffer with the biggest temperature variation used in PCR? It provides even the most experienced researchers with the means to understand the fundamental principles behind their preparation and use - an indispensable guide essential for everyone using buffers.
Table of Contents
1. Introduction and Basic Concepts. 2. Acids and Bases. 3. Theory of Buffer Action. 4. Measuring pH - the pH Meter. 5. Preparation of Buffer Solutions. 6. Automatic Buffer Calculations.
'a must for any cellular or molecular biologist'
- Cellular and Molecular Biology
' this book is a primer for the first and a refresher course for the second. It advises on which buffers to use, the effect of temperature and the consequences of ionic strength. What is most useful is that it explains biological buffers from first principles.' - Laboratory News