CRISPR-Cas9 as a Revolutionary Gene Editing Tool Genome Engineering, Advances and Applications in Agriculture

Preface

Principles and Practices of CRISPR-Cas9 Genome Editing Techniques

Seher Yerlikaya, Abdullah Aydın, Nisa Nur Yılmaz, Bayram Ali Yerlikaya, Musa Kavas and Izhar Ullah

Molecular Mechanisms and Current Progress of CRISPR/Cas in Plant Development

Pramod Sargar, Swapnil Baraskar, Muhammad Tanveer Altaf, Waqas Liaqat, Amna Jamil and Asia Maqbool

Genome Mapping and Gene Sequencing: Applications of CRISPR/Cas9 in Cereal Crops

Samar G. Thabet, Fatmah Ahmed Safhi, Amr Elkelish, Andreas Börner, Murad Muhammad, Mona FA Dawood and Ahmad M. Alqudah

CRISPR/Cas Design, DNA Repair Pathways, and Mutation Analysis

Abdullah Aydın, Seher Yerlikaya, Nisa Nur Yılmaz, Izhar Ullah, Bayram Ali Yerlikaya, Musa Kavas and Abdul Basit

CRISPR/Cas9 as an Effective Tool Used for the Improvement of Fruit Crops

Zain ul Abideen, Laiba Chaudhry, Mohsin Ali, Yaqoob Sultan and Abdul Basit

Ornamental Crops Improvement Using CRISPR/Cas9

Nouraiz Ahmed Tanveer, Momna Mehmood, Zain Ul Abadin, Noor ul Ain and Abdul Basit

Manipulating the CRISPR/Cas Genome Editing Technology for Targeted Genome Mutagenesis in Ornamental Plants

Irum Saadia Khan, Khurram Shahzad, Fahim Ullah Khan, Abdul Basit, Yaqoob Sultan and Attiq ur Rehman

CRISPR/Cas9 Technology in Engineering Viral and Bacterial Immunity in Agricultural Crops

Murad Muhammad, Shumaila Batool, Aqsa Batool, Matoor Mohsin Gilani, Ibad Ullah, Abdul Wahab, Ahmad M. Alqudah, Samar G. Thabet and Mona FA Dawood

Defence Mechanisms Developed by CRISPR/Cas in Plants Against Abiotic and Biotic Stresses

Laiba Chaudhry, Pawan Kumar Verma, Ravindra Solanki, Yaqoob Sultan, Murad Muhammad and Heba I. Mohamed

Trends in CRISPR/Cas9 Editing Technology as an Approach for Climate-Smart Agriculture

Samina Yasmin, Amjad Ali, Muhammad Tatar and Jaweria Iqbal

CRISPR/Cas9: An Innovative Biotechnological Approach for the Improvement of Oilseeds

Muhammad Tanveer Altaf, Waqas Liaqat, Amna Jamil, Muhammad Faheem Jan, Asia Maqbool, Naveed Abbas and Faheem Shehzad Baloch

Improvement of Vegetable Crops Using CRISPR-Cas9 as A Gene Editing Tool

Dilawar Khan, Kumail Ali Rizvi, Asim Gulzar and Tayyaba Zainab

CRISPR-Cas Based Genome Editing Strategies Towards Adaptation of Rice Crop to Disease Resistance

Irfan Ullah and Tariq Mahmood

Advancement in CRISPR/Cas9 Genome Editing in Field of Crop Improvement

Husnain Rauf

Linking miRNAs and Nano-technology with CRISPR/Cas System for Improvement of Agricultural Crops

Dilawar Khan, Asim Gulzar and Sobia Khaliq

Legal, Ethical, and Social Issues Remained in CRISPR/Cas Genome Editing Technology

Sana Ullah

Index

About the Editors

Biography

Abdul Basit is a Ph.D. researcher at the Department of Horticultural Science, Kyungpook National University, South Korea. He previously worked at the National Agricultural Research Center, Islamabad, and the Climate Change Center, The University of Agriculture, Peshawar. Supported by Pakistani and Korean government scholarships, he has published 67+ articles and 17+ book chapters. He has participated in over 35 scientific events. His research focuses on molecular cytogenetics, plant genomics, breeding, stress physiology, and horticultural crop production.

Heba I. Mohamed is a Professor of Plant Physiology at Ain Shams University. Her research focuses on plant stress physiology, biochemistry, eco-friendly stress alleviation, and genetic diversity. She has edited nine books published by international publishers and has also published 40 book chapters, eight reviews, and over 140 research articles. She obtained Encouragement State Award in Basic Sciences (Biological Sciences) 2018 and has been listed among Stanford–Elsevier’s top 2% most influential scientists (2021–2024).

Syed Tanveer Shah is an Assistant Professor at the Department of Agriculture, Hazara University, and previously served over a decade in Horticulture at The University of Agriculture, Peshawar. He holds B.Sc (Hons), M.Sc (Hons), and Ph.D. degrees in Horticulture. Listed among H-index–based scientists (2022–23), he has 60+ research articles, and 8+ book chapters. He has participated in 20+ scientific events. His expertise includes pomology, postharvest science, stress physiology, plant genomics, and horticultural crop production.

Mona FA Dawood is an Associate Professor at the Botany and Microbiology Department, Assiut University, Egypt. She earned her M.Sc and Ph.D. in abiotic stress and has published 50+ research articles, 5 reviews, 9 book chapters, and 10 conference abstracts. With an H-index of 21, she serves as editor and guest editor for reputed journals and has reviewed over 210 articles. In 2023, she was recognized among Stanford University’s top 2% scientists worldwide.