1st Edition
Cancer Detection and Diagnosis A Handbook of Emerging Technologies
Preface
List of Contributors
Overview of Cancer Features and Impact
Miguel Ossandon, Ben Prickril, and Avraham Rasooly
Section 1 Optical technologies
1 Near-Infrared Macroscopic and Mesoscopic Fluorescence Lifetime FRET Imaging to Measure
Intra-Tumor Heterogeneity of Antibody-Target Engagement
Amit Verma, Saif Ragab, Shan Gao, Catherine Sherry, Nanxue Yuan, Vikas Pandey, Xavier Intes, and Margarida Barroso
2 Combined Reflectance Confocal Microscopy–Optical Coherence Tomography for Skin Cancer
Detection and Therapy Guidance
Nicusor Iftimia, John Grimble, Gopi Maguluri, Aditi Sahu, Miguel Cordova, and Milind Rajadhyaksha
3 In Vivo Confocal Laser Endomicroscopy: An Imaging Biomarker for Risk Stratification of Precancerous
Pancreatic Cystic Lesions
Da Yeon Ryoo, Bryn Koehler, Vanisha Patel, and Somashekar G. Krishna
4 Portable Confocal Microscopy for Aiding Diagnosis and Treatment of Skin Cancer
Momoka Sugimura, Jingwei Zhao, Kenneth Marcelino, Yongjun Kim, Rafael Romero, and Dongkyun Kang
5 Quantitative Fluorescence Imaging, Light-Triggering, and Monitoring of Chemodrug Release from
Liposomes In Vivo by a Mesoscopic-Scale Theranostic Endoscope
Ulas Sunar, Rasel Ahmmed, Eli Kluiszo, and Semra Aygun-Sunar
6 Fluorescence Imitating Brightfield Imaging (FIBI): A Simple Slide-Free Microscopy Approach
Nathan Anderson, Farzad Fereidouni, and Richard Levenson
7 Fluorescently Labeled Tyrosine Kinase Inhibitors for Intracellular Protein Target
Allison Solanki, Jesse Korber, Summer L. Gibbs, and Lei G. Wang
8 Real-Time Cancer Metabolism Detection Using a Nanocoil Integrated Hyperpolarized Micromagnetic Resonance Spectrometer
Thasin Peyear, Roberta Pigliapochi, and Kayvan R. Keshari
9 Detection of TMEM Doorways and Their Activity Required for Metastasis
George S. Karagiannis, Robert J. Eddy, Joseph A. Sparano, Jesus Anampa Mesias, Thomas E. Rohan, Craig Branch, John S. Condeelis, David Entenberg, and Maja H. Oktay
10 Reading the Reader: Utilizing Eye Movements and Machine Learning to Enhance Accuracy during Diagnostic Visual Search
Gregory J. DiGirolamo, Federico Sorcini, Leo Joskowicz, Yakir Winograd, Assaf Hoog, and Max P. Rosen
11 Optical Imaging Technology for In Vivo Tumor Detection
Radka Bokorova, Duy Binh Tran, and Tohru Yamada
12 Adapting Image Foundational Model to Identify Tumor Budding from H&E Images in Colorectal Cancer Diagnostics
Ziyu Su, Usama Sajjad, Wendy L. Frankel, Metin N. Gurcan, Wei Chen, and Muhammad Khalid Khan Niazi
13 Quantitative Phase Imaging for Assessing Tumor Cell Adaptability
Rebecca G. Zitnay, Shukran Alizada, Anastasia Prokofyeva, Thomas A. Zangle, and Robert L. Judson-Torres
14 An Exo-PROS Biosensor Simultaneously Detects Tumor-Derived Exosomal Protein-MicroRNA Pairs for Lung and Breast Cancer Diagnosis
Chang-Chieh Hsu and Yun Wu
Section 2 Imaging Technologies
15 Epigenetic Tools for Guiding Low Dose Computerized Tomography (LDCT) Screening Decision-Making
Winter Philibert and Robert Philibert
16 Multi-View Models for Colorectal Polyp Detection in CT Colonography
Mohamed Yousuf, Samir Harb, Asem Ali, Salwa Elshazly, and Aly Farag
17 Hybrid Multi-Dimensional MRI of Prostate Cancer
Aritrick Chatterjee, and Aytekin Oto
18 Detection and Characterization of Brain Metastases Using Quantitative Chemical Exchange Saturation Transfer MR Fingerprinting (CEST-MRF)
Ouri Cohen
19 Narrow-Beam CT: A Solution to the Limitations in Breast Cancer Screening
Peymon Ghazi
20 Broadband Coherent Anti-Stokes Raman Scattering Microscopy for Metabolic and Phenotypic Imaging in Cancer
Jessica Z. Dixon, Wei-Wen Chen, Rajas Poorna, Xavier Audier, and Marcus T. Ciceronex
21 Spatial Multiplexed Immunofluorescence Imaging in Exploring Tumor Immune Microenvironment of Melanoma
Jun Jiang, Shankar Suman, Caitlin Ward, Raymond Moore, Zachary Fogarty, Alexey Leontovich, Wendy Nevala, Chathu Atherton, Jill Schimke, and Svetomir Markovic
22 Spectroscopic Optical Coherence Tomography for In Situ Analysis of Colonic Epithelium
Jatin Roper, Wesley Kendall, and Adam Wax
23 Analysis of Circulating Tumor DNA (ctDNA) by Digital Droplet PCR (ddPCR)
Bryant L. McCue and George Miles
24 Noninvasively Monitoring the Delivery of DNA Alkylating Agents and Cellular Responses to These Agents Using Chemical Exchange Saturation Transfer Magnetic Resonance Imaging
Ethel J. Ngen
Section 3 Nucleic Acid Technologies
25 Clonal Analysis of Cancer by Mitochondrial DNA Barcoding
Jonathan Good, Ksenia R. Safina, and Peter van Galen
26 Implementing Clinical Risk Assessment for Genetic Susceptibility to Cancer: Challenges and Successes in a Statewide Initiative
Sarah Austin, Liliana Arida-Moody, Erika Hanson, Shayna Weiner, Matthew Demerath, Emerson Delacroix, Erika Koeppe, Ken Resnicow, Jennifer J. Griggs, and Elena M. Stoffel
27 Assessing Tumor Tissue for the Alternative Lengthening of Telomeres (ALT) Phenotype
C. Patrick Reynolds, Trevor A. Burrow, and Balakrishna Koneru
28 Click Chemistry-Mediated Enrichment of Tumor-Derived Extracellular Vesicles for RNA-Based Digital Scoring
Yazhen Zhu, Jacqueline Ziqian Yang, Anna Yaxuan Wei, Vivian Xufei Zuo, Judy Law, Sungyong You, Ju Dong Yang, Vatche G. Agopian, Edwin Posadas, Junseok Lee, and Hsian-Rong Tseng
29 Identifying Methods to Deliver Mutant p53-Based Diagnostics and Therapeutics to Target the Previously
Gu Xiao, Outi Keinänen, Florencia Madorsky Rowdo, Samantha Delaney, Katherine W. Harmon, Brian M. Zeglis, and Jill Bargonetti
30 DNA Methylation Biomarkers for Preoperative Diagnostic of Thyroid Nodules
Byung-Wook Kim, Yuman Fong, John H. Yim, and Maria A. Hahn
31 Utilizing RADAR for the Integration of Genomic Data and Immunotherapy Targets in Precision Oncology for Multiple Myeloma
Alessandro Laganà, Sanjeev Sariya, David T. Melnekoff, Meghana Ram, and Samir Parekh
32 Nanopore Whole Transcriptome Sequencing Offers the Potential for Accessible Classification of Pediatric Cancers
Thomas B. Alexander and Jeremy R. Wang
33 Quantitative Detection of Cancer Nucleic Acid Biomarkers with a Selective Solid-State Nanopore Assay
Adam R. Hall, Sara Abu Jalboush, Ian D. Wadsworth, Mohamed Amin Elaguech, and Komal Sethi
34 Isolation and Characterization of Cell-Free RNA from Liquid Biopsy Taken from Cancer Patients
Real Sumayya, Abdul Sattar, Luisa Quezada Ziesse, and Subhajyoti De
Section 4 Biomarker–Molecular Technologies
35 The Use of Dielectrophoresis to Recover Cancer-Derived Nanoparticles Straight from Undiluted Human
Plasma for Cancer Detection Applications
Delaney Shea, Jason Warea, Michael Henderson, Ella Stimson, Mehrzad Sasanpour, Sarah Mitchell, Christian Ross, Shelby Nicholas, and Stuart D. Ibsen
36 Conditional Reprogramming: A Living Biomarker and Phenotypic Screening Drug Platform for Urological Cancer
Danyal Daneshdoust, Abdul Qawee Rani, Peng Wang, Shang-jui Wang, Akshay Sood, Debasish Sundi, Qi-en Wang, Zaibo Li, Anil V. Parwani, Nagireddy Putluri, Cheryl Lee, Jenny Li, and Xuefeng Liu
37 Proteolytic Activity Signatures as Candidate Biomarkers for Thyroid Cancer
Danielle E. Dettling and Giselle M. Knudsen
38 Carbohydrate Microarrays Identify the Stage-Specific Embryonic Antigen (SSEA)-0 as a Novel Oncofetal Cancer Marker
Denong Wang
39 Collective Attributes of Extracellular Vesicles as Biomarkers for Cancer Detection
Alfred Akinlalu, Tommy Gao, Sky Gao, and Dali Sun
40 Using a Gamified Mobile App Approach to Train Tobacco Control Program Implementers in Schools in India
Eve M. Nagler, Chuck Sigmund, Smita P. Warke, Priyanka Ghosh, Paromita Mehta, Leah C. Jones, and Mangesh Pednekar
41 Parallel Reaction Monitoring (PRM) Quantitative Analysis of Glycopeptide Biomarkers from Patient Serum
Komal Abhange, Yu Lin, Jie Zhang, Jianhui Zhu, Amit G. Singal, Neehar D. Parikh, and David M. Lubman
42 Point-of-Care Technologies for Molecular Subtyping of Breast Cancer in Low- and Middle-Income Countries ��� 387
Wenting Gao, Iftak Hussain, Sally Adebamowo, David Erickson, and Clement Adebamowo
43 Development of LLM for Prostate Cancer—The Need for Domain-Tailored Training
Amara Tariq, Man Luo, Aisha Urooj, Avisha Das, Jiwoong Jeong, Shubham Trivedi, Haidar Abdul-Muhsin, Umer
Ghaffar, Nathan Yu, Bhavik Patel, and Imon Banerjee
44 Biosample and Method Selection for Marker Studies
Angela J. Yoon
45 Subharmonic-Aided Pressure Estimation for Evaluating Cancer
Hailee Mayer, Kibo Nam, Jason Shames, and Flemming Forsberg
46 Development and Implementation of a Method for Registering Prostate-Specific Antigen (PSA) Biosensor-Based Assay Results in a Personalized QR Code
Maria Daoutakou1 and Spyridon Kintzios
Section 5 Organel and Cellular Technologies
47 Deep Learning-Based Colon Segmentation for Accurate Colorectal Polyps Detection
Samir Harb, Mohamed Yousuf, Ahmed Elsayed, Asem Ali, Salwa Elshazly, and Aly Farag
48 Emerging Blood-Based Tests for Colorectal Cancer Screening: Advances, Challenges, and Future Directions
Gloria D. Coronado, Jennifer L. Schneider, Cheryl A. Johnson, and Leslie Bienen
49 Microfluidic Isolation and Molecular Characterization of Circulating Tumor Cells in Prostate Cancer
Rea Pittie, Victor Putaturo, Shyamala Maheswaran, Daniel A. Haber, Mehmet Toner, Avanish Mishra, and David T. Miyamoto
50 Chemical Imaging for Next-Generation Histopathology
Kianoush Falahkheirkhah, Kevin Yeh, and Rohit Bhargava
51 GALAD Score and Extracellular Vesicle-Based Assays for Early Detection of Hepatocellular Carcinoma
Yi-Te Lee, Yazhen Zhu, Sungyong You, Vatche G. Agopian, Hsian-Rong Tseng, and Ju Dong Yang
52 Using Epidemiologic Research Methods to Adapt a Human Papillomavirus Assay for Cervical Cancer Screening in Low- and Middle-Income Countries
Louise Kuhn, Rakiya Saidu, and Lynette Denny
53 Organoid Models for Early Detection and Diagnosis of Colorectal Cancer
Jinghui Liang, Janvhi Suresh Machhar, and Chengyu Liang
Section 6 Computaional – Digital Technologies
54 Experimental Methods for Establishing and Maintaining Canine Bladder Cancer Organoids
Mohamed Elbadawy, Christopher Zdyrski, Hannah Wickham, Corey F. Saba, Savantha Thenuwara, Alison P. Mosichuk, Dipak Kumar Sahoo, Jonathan P. Mochel, and Karin Allenspach
55 Top-Down Proteomics of Cancer Cells by Advanced Capillary Electrophoresis–Mass Spectrometry
Tian Xu and Liangliang Sun
56 X-ray Diffraction Imaging for Cancer Detection and Diagnosis
Joel A. Greenberg and Anuj J. Kapadia
57 Restriction Spectrum Imaging (RSI) Models for the Detection of Female Cancers
Aida Haddad, Summer J. Batasin, Sabrina Kohanzad, Emily Barnard, Ana E. Rodríguez-Soto, Stéphane Loubrie, and Rebecca Rakow-Penner
Index
Biography
Miguel Ossandon has a dual background in clinical laboratory and computer science. He started working in cancer research at the Lombardi Cancer Center at Georgetown University where he also began his undergraduate training in computer science. He has been working for the National Cancer Institute since 2007. Miguel received his master’s degree at the George Washington University and PhD in computer science at the University of Maryland Baltimore County. As a program director in the Diagnostic Biomarkers and Technology Branch, he manages a grant portfolio related to computational modeling and machine learning approaches for cancer diagnosis, digital image processing/analysis, and microfluidic and circulating tumor cell technology.
Ben Prickril is a former US government official working at the US National Institutes of Health, National Cancer Institute. He has a background in medically related chemistry, microbiology, immunology, patenting and intellectual property, and global health. International research development includes experience in France, Turkey, Ukraine, Czech Republic and Burkina Faso. He received his PhD from the University of Georgia.
Avraham Rasooly is in the Division of Cancer Treatment and Diagnosis, National Cancer Institute. He has been responsible for developing research programs on new approaches for cancer therapy, including technologies for microbial-based cancer therapy. He received his PhD from Michigan State University.
