1st Edition

Capillary Electrophoresis of Proteins




ISBN 9780824702052
Published October 20, 1998 by CRC Press
300 Pages

USD $300.00

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Book Description

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."

Table of Contents

Principles and practice of capillary electrophoresis: basic concepts; brand broadening in capillary electrophoresis; capillary electrophoresis intrumentation; preparative capillary electrophoresis; capillary electrophoresis separation modes. Detection of proteins: absorbance detection; fluorescence detection; mass spectrometry. Strategies for reducing protein-wall interactions: operations at pH extremes; use of buffer additives; capillary coatings and other surface modifications. Capillary zone electrophoresis: developing a CZE method; sample preconcentration techniques; applications. Capillary isoelectric focusing: sample preparation and injection; focusing; two-step CIEF; single-step CIEF; capillary selection; detection; optimizing CIEF analysis; capillary IEF as a micro-preparative technique; applications; future developments. Sieving separation: analysis of native proteins; analysis of SDS-protein complexes. Concluding remarks.

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Reviews

". . .well written and referenced. It is one of the most comprehensive texts currently available on the application of capillary electrophoresis to protein analysis. . ..a useful source of information for separation scientists and postgraduate students wishing to obtain an overall review of protein capillary electrophoresis separations."
---European Journal of Pharmaceutics and Biopharmaceutics