Cell Culture Technology for Pharmaceutical and Cell-Based Therapies  book cover
1st Edition

Cell Culture Technology for Pharmaceutical and Cell-Based Therapies

ISBN 9780824753344
Published August 30, 2005 by CRC Press
788 Pages 23 Color & 102 B/W Illustrations

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Book Description

Edited by two of the most distinguished pioneers in genetic manipulation and bioprocess technology, this bestselling reference presents a comprehensive overview of current cell culture technology used in the pharmaceutical industry. Contributions from several leading researchers showcase the importance of gene discovery and genomic technology development in the production of biotechnology products, tissue engineering, and cell-based therapies. Offering detailed guidance, they cover the steps leading up to the creation of viable therapies including host cell selection, cloning and gene amplification, bioreactor design and operation, protein purification, optimization, scale-up, and facility design.

Table of Contents

Preface. Cell Culture Technology: An Overview. Recombinant DNA Technology and Cell Line Development. Medium Development. Cell Metabolism. Protein Glycosylation. Cell Culture Bioreactors. Aeration, Mixing and Hydrodynamics in Bioreactors. Instrumentation and Process Control. Cell Culture Kinetics and Modeling. Fed-Batch Cultivation Of Mammalian Cells For Production Of Recombinant Proteins. Optimization of High Density Perfusion Bioreactors. Cell Separation And Product Capture. Downstream Processing. Formulation, Finishing, and Packaging. Validation Of Cell Culture Based Processes & Qualification Of Associated Equipment And Facility. Facility Design. Transient Expression of Proteins. Large-Scale Propagation of Insect Cells. Advances In Adult Stem Cell Culture. Ex Vivo Culture Of Hematopoietic And Mesenchymal Stem Cells For Tissue Engineering And Cell-Based Therapies.

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"…The editors have brought together an outstanding group of experts from both private industry and academia….recommend[ed]."
-Doody's Reviews