204 Pages
118 B/W Illustrations
by
Taylor & Francis
204 Pages
118 B/W Illustrations
by
Taylor & Francis
Also available as eBook on:
Upstream processing refers to the production of proteins by cells genetically engineered to contain the human gene which will express the protein of interest. The demand for large quantities of specific proteins is increasing the pressure to boost cell culture productivity, and optimizing bioreactor output has become a primary concern for most pharmaceutical... Read more
1. Cell Line Development and Culture Strategies: Future Prospects to Improve Yields 2. Use of DNA Insulator Elements and Scaffold / Matrix-Attached Regions for Enhanced Recombinant Protein Expression 3. Targeted Gene Insertion to Enhance Protein Production From Cell Lines 4. Recombinant Human IgG Production From Myeloma and Chinese Hamster Ovary Cells 5. Cell Culture Media Development: Customization of Animal Origin-Free Components and Supplements 6. Post-Translational Modification of Recombinant Proteins 7. Metabolic Engineering to Control Glycosylation 8. An Alternative Approach: Humanization of N-glycosylation Pathways in Yeast 9. Perfusion or Fed-Batch? A Matter of Perspective
Biography
Michael Butler-Associate Dean of Science; Professor of Cell Technology, Department of Microbiology, School of Science, University of Manitoba, Winnipeg, Canada
