Cell Electrophoresis: 1st Edition (Hardback) book cover

Cell Electrophoresis

1st Edition

By Johann Bauer

CRC Press

336 pages

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Hardback: 9780849389184
pub: 1994-09-09
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Description

This book presents a summary of the application and instrumentation of cell electrophoresis. The method of making cell purification and characterization possible according to the cellular negative surface charge density is discussed, and ideas for future developments are explained. The negative electrostatic forces at cell surfaces provide information about cell-cell interaction, blood vessel sealing, cytokine actions, cell transformation, ion transport phenomena, and other biological phenomena. Recalculations of the physical principles of cell electrophoresis reveal possibilities for removing disruptive factors caused by electrical current, heat, and sedimentation. The introduction of computer technology, the performance of simultaneous two-parameter measurements, and the application of cell-friendly but current-inert buffer systems render the method more reliable and efficient.

Table of Contents

Theoretical Considerations:

Contributions to a Mathematical Theory of Free Flow Electrophoresis, F.G. Boese

Numerical Description of Zone Electrophoresis in the CFE Device, T.M. Grateful, A.M. Athalye, and E.N. Lightfoot

Improvements of the Method:

Theoretical and Experimental Studies on the Stabilization of Hydrodynamic Flow in Free-Fluid Electrophoresis, P.H. Rhodes and R.S. Snyder

The Loading and Unloading of Cells in Electrophoretic Separations, P. Todd

Electrode Compartment Separating Membranes for Cell Electrophoresis, J. Heinrich and H. Wagner

Cell Electrophoresis Using Antibodies and Antigens as Ligands, H.H.P. Cohly and S.K. Das

Buffers and Their Effects on Cells:

New Buffer Systems for Cell Electrophoresis, K.A. Kinsley and L.S. Rodkey

Alteration of Cellular Features after Exposure to Low Ionic Strength Medium, I. Bernhardt

Electrophoresis of Cells at Physiologic Ionic Strength, M.V. Golovanov

The Importance of Determination of the Negative Surface Charge Density of Cells:

Simultaneous Two-Parameter Measurements of the Electrophoretic Features of Cell Populations and Their Sedimental Characteristics, G.G. Slivinsky

Cell Interactions and Surface Hydrophilicity: Influence of Lewis Acid-Base and Electrostatic Forces, C. J. van Oss

Electrical Surface Phenomena of Endothelial Cells, F.F. Vargas

Application of Cell Electrophoresis for Clinical Diagnosis, W. Schütt, N. Hashimoto, and M. Shimizu

The Negative Surface Charge Density of Cells and Their Actual State of Differentiation or Activation, J. Bauer

Cell Electrophoresis in Space:

Cell Electrophoresis in Microgravity: Past and Future, D.R. Morrison

Cell Electrophoresis in Microgravity, U. Friedrich, G. Ruyters, and J. Bauer

Subject Categories

BISAC Subject Codes/Headings:
MED008000
MEDICAL / Biochemistry
SCI017000
SCIENCE / Life Sciences / Cytology
SCI029000
SCIENCE / Life Sciences / Genetics & Genomics