Cell Membrane Nanodomains: From Biochemistry to Nanoscopy describes recent advances in our understanding of membrane organization, with a particular focus on the cutting-edge imaging techniques that are making these new discoveries possible. With contributions from pioneers in the field, the book explores areas where the application of these novel techniques reveals new concepts in biology. It assembles a collection of works where the integration of membrane biology and microscopy emphasizes the interdisciplinary nature of this exciting field.
Beginning with a broad description of membrane organization, including seminal work on lipid partitioning in model systems and the roles of proteins in membrane organization, the book examines how lipids and membrane compartmentalization can regulate protein function and signal transduction. It then focuses on recent advances in imaging techniques and tools that foster further advances in our understanding of signaling nanoplatforms. The coverage includes several diffraction-limited imaging techniques that allow for measurements of protein distribution/clustering and membrane curvature in living cells, new fluorescent proteins, novel Laurdan analyses, and the toolbox of labeling possibilities with organic dyes.
Since superresolution optical techniques have been crucial to advancing our understanding of cellular structure and protein behavior, the book concludes with a discussion of technologies that are enabling the visualization of lipids, proteins, and other molecular components at unprecedented spatiotemporal resolution. It also explains the ins and outs of the rapidly developing high- or superresolution microscopy field, including new methods and data analysis tools that exclusively pertain to these techniques.
This integration of membrane biology and advanced imaging techniques emphasizes the interdisciplinary nature of this exciting field. The array of contributions from leading world experts makes this book a valuable tool for the visualization of signaling nanoplatforms by means of cutting-edge optical microscopy tools.
Protein and Lipid Nanodomains
Giant Unilamellar Vesicles (GUVs) as a Laboratory to Study Mesoscopic Lipid Domains in Membranes, Luis A. Bagatolli and Ole G. Mouritsen
An Active Basis for the Nanoscopic Organization of Membrane Components in Living Cell Membranes, Subhasri Ghosh and Satyajit Mayor
Functional Role of Membrane Lipids in EGF Receptor Dynamics and Regulation, Michal Grzybek, Theresia Gutmann, and Ünal Coskun
Tetraspanins as Master Organizers of the Plasma Membrane, Cindy K. Miranti, Alexis Bergsma, and Annemiek B. van Spriel
B Cell Receptor Signaling, Christoph Feest, Andreas Bruckbauer, and Facundo D. Batista
Imaging the Complexity, Plasticity, and Dynamics of Caveolae, Asier Echarri and Miguel A. Del Pozo
Membrane Microdomains Enriched in Ceramides: From Generation to Function, Sibylle Schneider-Schaulies, Nora Müller, and Erich Gulbins
Domains of Phosphoinositides in the Plasma Membrane, Geert van den Bogaart and Martin ter Beest
Signaling Phagocytosis: Role of Specialized Lipid Domains, Sharon Eisenberg and Sergio Grinstein
Advanced Ensemble Imaging Techniques
Fluctuation Spectroscopy Methods for the Analysis of Membrane Processes, Michelle A. Digman
Spatial Intensity Distribution Analysis (SpIDA): A Method to Probe Membrane Receptor Organization in Intact Cells, Antoine G. Godin, Benjamin Rappaz, Laurent Potvin-Trottier, Yves De Koninck, and Paul W. Wiseman
Live-Cell TIRF Imaging of Molecular Assembly and Plasma Membrane Topography, Adam D. Hoppe and Shalini T. Low-Nam
Expanding the Fluorescence Toolbox
Laurdan Identifies Different Lipid Membranes in Eukaryotic Cells, Enrico Gratton and Michelle A. Digman
Development of Optical Highlighter Fluorescent Proteins and Their Applications in Super-Resolution Fluorescence Microscopy, Yan Fu and George H. Patterson
Targeting Dyes for Biology, Saumya Saurabh and Marcel P. Bruchez
Combined Topography, Recognition, and Fluorescence Measurements on Cells, Rong Zhu, Memed Duman, Josef Madl, Gerhard J. Schütz, and Peter Hinterdorfer
Super-Resolution Imaging with Single-Molecule Localization, Anna Oddone, Ione Verdeny Vilanova, Johnny Tam, Štefan Bálint, and Melike Lakadamyali
Visualization and Resolution in Localization Microscopy, Robert P.J. Nieuwenhuizen, Sjoerd Stallinga, and Bernd Rieger
Molecular Plasma Membrane Dynamics Dissected by STED Nanoscopy and Fluorescence Correlation Spectroscopy (STED-FCS), Christian Eggeling and Alf Honigmann
Nanophotonic Approaches for Nanoscale Imaging and Single-Molecule Detection at Ultrahigh Concentrations, Mathieu Mivelle, Thomas S. van Zanten, Carlo Manzo, and Maria F. Garcia-Parajo