1st Edition

Clinical Biochemistry A Laboratory Guide

    306 Pages 73 Color & 74 B/W Illustrations
    by Jenny Stanford Publishing

    306 Pages 73 Color & 74 B/W Illustrations
    by Jenny Stanford Publishing

    A knowledge of the practical aspects of clinical biochemistry is essential for medical students to understand the diagnostic and prognostic status of any ailment. This book addresses and compiles some of the invariably used protocols in biochemistry with the aim to enhance the understanding of the basics behind following the methodologies, including those for urine analysis; carbohydrate, protein, and lipid analyses; and diabetes and lipid profile. It discusses organ function tests, such as liver, renal, or pancreatic function tests, in addition to exploring pH and buffer, colorimeter, spectrophotometer, chromatography, electrophoresis, enzyme-linked adsorbent assay, radioimmunoassay, biosensor/glucometer, fructosamine, DNA isolation, mass spectrometry, flow cytometry, centrifugation, automation, cerebrospinal fluid examination, and oxalic acid test. The book also touches upon the basics of laboratory safety rules and laboratory glassware.

    The book is helpful for students to understand the relationship between quality management and specimen collection and processing them for their biological functions. It is well-suited for academic courses, including undergraduate, graduate, postgraduate, and professional courses and covers the practical syllabus for MBBS, BDS, BSc MLT, BSc nursing, and MSc students. For undergraduate students in the disciplines of biochemistry, molecular biology, chemistry, and other related biological sciences, the book forms an integral part of the syllabus with its case studies, MCQs, biochemistry, OSPEs, etc. It also demonstrates the actual experiments for understanding the concepts better and is a must read for students to acquire skills to work with a clinical biochemistry laboratory.

    1. Lab Safety Rules

    2. Specimen Collection and Processing

    3. Quality Management in the Laboratory

    4. Urine analysis

    5. Carbohydrates

    6. Blood Glucose

    7. Proteins

    8. Lipid Analysis

    9. Lipid Profile

    10. Liver Function Test

    11. Renal Function Test

    12. Pancreatic Function Test

    13. Acids and Bases

    14. Colorimeter

    15. Chromatography

    16. Electrophoresis

    17. Enzyme-Linked Adsorbent Assay

    18. Radioimmunoassay

    19. Polymerase Chain Reaction

    20. Biosensors/Glucometer

    21. Glycated Hemoglobin

    22. Enzymes

    23. DNA Isolation from Blood and Tissue

    24. Mass Spectrometry

    25. Flow Cytometry

    26. Centrifugation

    27. Automation

    28. Cerebrospinal Fluid Examination

    29. Oxalic acid


    Rooma Devi is an assistant professor at the Department of Biochemistry, MM Institute of Medical Sciences & Research, Maharishi Markandeshwar (deemed to be university) [MM(DU)], Mullana, Ambala, India. She has worked as a demonstrator in the Department of Biochemistry at Pt. B. D. Sharma PGIMS, Rohtak, India, and as a Fastrack Young Scientist at the Department of Science and Technology, India. Her current research interests include the development of laboratory models, biosensors technology, nanotechnology, enzyme technology, and clinical biochemistry.

    Aman Chauhan is an assistant professor at the Department of Biochemistry, MM Institute of Medical Sciences & Research, MM(DU). He has worked as a senior resident in the Department of Biochemistry at Atal Bihari Vajpayee Institute of Medical Sciences and Dr Ram Manohar Lohia Hospital as well as Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India. His current research focuses on biochemical biomarkers, including interleukins and nanocomposites-based sensors, and Alzheimer’s and Parkinson’s diseases on animal models.

    Simmi Kharb is a senior professor and head of the Department of Biochemistry at Pt. B. D. Sharma PGIMS, University of Health Sciences, Rohtak, India. She is also the nodal officer at the DHR-ICMR-Multidisciplinary Research Unit at the university. She is part of several prestigious research projects, including Ayushman Bharat–Pradhan Mantri Jan Arogya Yojana (AB-PMJAY), multicentric research projects from MRUs, and Dr Padam Singh Research and Development Scheme from DHR-ICMR.

    Chandra Shekhar Pundir is an emeritus professor of CSIR at the Department of Biochemistry, Maharshi Dayanand University (MDU), Rohtak, India. He has been a professor and the founder head of the Department of Biochemistry and also the dean of the Faculty of Life Sciences at MDU. He holds 7 Indian patents and 1 international (PCT) patent in the fields of enzyme technology and biosensor technology. He is at the 245th rank in the Top 2% Scientists in Biotechnology listed by Stanford University, USA. His current research areas are analytical/clinical biochemistry, enzyme technology, biosensor technology, and bionanotechnology.