1st Edition
Clinical Biochemistry A Laboratory Guide
A knowledge of the practical aspects of clinical biochemistry is essential for medical students to understand the diagnostic and prognostic status of any ailment. This book addresses and compiles some of the invariably used protocols in biochemistry with the aim to enhance the understanding of the basics behind following the methodologies, including those for urine analysis; carbohydrate, protein, and lipid analyses; and diabetes and lipid profile. It discusses organ function tests, such as liver, renal, or pancreatic function tests, in addition to exploring pH and buffer, colorimeter, spectrophotometer, chromatography, electrophoresis, enzyme-linked adsorbent assay, radioimmunoassay, biosensor/glucometer, fructosamine, DNA isolation, mass spectrometry, flow cytometry, centrifugation, automation, cerebrospinal fluid examination, and oxalic acid test. The book also touches upon the basics of laboratory safety rules and laboratory glassware.
The book is helpful for students to understand the relationship between quality management and specimen collection and processing them for their biological functions. It is well-suited for academic courses, including undergraduate, graduate, postgraduate, and professional courses and covers the practical syllabus for MBBS, BDS, BSc MLT, BSc nursing, and MSc students. For undergraduate students in the disciplines of biochemistry, molecular biology, chemistry, and other related biological sciences, the book forms an integral part of the syllabus with its case studies, MCQs, biochemistry, OSPEs, etc. It also demonstrates the actual experiments for understanding the concepts better and is a must read for students to acquire skills to work with a clinical biochemistry laboratory.
1. Lab Safety Rules
2. Specimen Collection and Processing
3. Quality Management in the Laboratory
4. Urine analysis
5. Carbohydrates
6. Blood Glucose
7. Proteins
8. Lipid Analysis
9. Lipid Profile
10. Liver Function Test
11. Renal Function Test
12. Pancreatic Function Test
13. Acids and Bases
14. Colorimeter
15. Chromatography
16. Electrophoresis
17. Enzyme-Linked Adsorbent Assay
18. Radioimmunoassay
19. Polymerase Chain Reaction
20. Biosensors/Glucometer
21. Glycated Hemoglobin
22. Enzymes
23. DNA Isolation from Blood and Tissue
24. Mass Spectrometry
25. Flow Cytometry
26. Centrifugation
27. Automation
28. Cerebrospinal Fluid Examination
29. Oxalic acid
Biography
Rooma Devi is an assistant professor at the Department of Biochemistry, MM Institute of Medical Sciences & Research, Maharishi Markandeshwar (deemed to be university) [MM(DU)], Mullana, Ambala, India. She has worked as a demonstrator in the Department of Biochemistry at Pt. B. D. Sharma PGIMS, Rohtak, India, and as a Fastrack Young Scientist at the Department of Science and Technology, India. Her current research interests include the development of laboratory models, biosensors technology, nanotechnology, enzyme technology, and clinical biochemistry.
Aman Chauhan is an assistant professor at the Department of Biochemistry, MM Institute of Medical Sciences & Research, MM(DU). He has worked as a senior resident in the Department of Biochemistry at Atal Bihari Vajpayee Institute of Medical Sciences and Dr Ram Manohar Lohia Hospital as well as Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India. His current research focuses on biochemical biomarkers, including interleukins and nanocomposites-based sensors, and Alzheimer’s and Parkinson’s diseases on animal models.
Simmi Kharb is a senior professor and head of the Department of Biochemistry at Pt. B. D. Sharma PGIMS, University of Health Sciences, Rohtak, India. She is also the nodal officer at the DHR-ICMR-Multidisciplinary Research Unit at the university. She is part of several prestigious research projects, including Ayushman Bharat–Pradhan Mantri Jan Arogya Yojana (AB-PMJAY), multicentric research projects from MRUs, and Dr Padam Singh Research and Development Scheme from DHR-ICMR.
Chandra Shekhar Pundir is an emeritus professor of CSIR at the Department of Biochemistry, Maharshi Dayanand University (MDU), Rohtak, India. He has been a professor and the founder head of the Department of Biochemistry and also the dean of the Faculty of Life Sciences at MDU. He holds 7 Indian patents and 1 international (PCT) patent in the fields of enzyme technology and biosensor technology. He is at the 245th rank in the Top 2% Scientists in Biotechnology listed by Stanford University, USA. His current research areas are analytical/clinical biochemistry, enzyme technology, biosensor technology, and bionanotechnology.
