Published on behalf of the Biological Stain Commission
For 75 years Conn's Biological Stains has been a standard reference for all those who used dyes and colorants in the biological and medical sciences. This long awaited tenth edition appears 25 years after R.D. Lillie's ninth and has been completely rewritten to reflect the increase in range of uses. Although the staining of microscopical preparations continues to expand the uses of dyes and fluorochromes now extend far beyond this traditional application.
This book provides the first critical overview of the whole range of low molecular weight fluorescent probes, outside the catalogue literature. The first ten chapters are essays, by leading experts, on the important aspects of colorants and their uses. Most of the remainder of the book consists of descriptions by Dr Horobin of the properties and recent applications of hundreds of individual compounds, in about twenty chemical classes. The last chapter reviews the procedures employed at the Biological Stain Commission's laboratory to assay and test dyes and certify them as suitable for their intended applications.
Table of Contents
Ch 1. Introduction to dyes and stains, F.H. Kasen. Ch 2. The history of staining, B. Bracegirdle. Ch 3. Nomenclature and classification of dyes and other coloring agents, J.A. Kiernan. Ch 4. Applications of dyes, fluorochromes and pigments, M. Wainwright. Ch 5. Mechanisms of biological staining, R.W. Horobin. Ch 6. Dye purity and dye standardisation for biological staining, H. Lyon. Ch 7. Reactive staining reagents and fluorescent labels, J.C. Stockert. Ch 8. The use of dyes and fluorochromes as indicators, M. Wainwright. Ch 9. Nitroso and nitro dyes, R.W. Horobin. Ch 10. Monoazo dyes, R.W. Horobin. Ch 11. Dis-, tris- and poly azo dyes, R.W. Horobin. Ch 12. Diazonium salts and their reaction products with coupling agents, R.W. Horobin. Ch 13. Tetrazolium salts and formazans, R.W. Horobin. Ch 14. Amino di- and triarylmethane dyes, R.W. Horobin. Ch 15. Hydroxy triarylmethanes, R.W. Horobin. Ch 16. Xanthenes, R.W. Horobin. Ch 17. Acridines and phenanthridines, R.W. Horobin. Ch 18. Azines, R.W. Horobin. Ch 19. Oxazines and related dyes, R.W. Horobin. Ch 20. Thiazines, R.W. Horobin. Ch 21. Romanowsky-Giemsa stains, D.H. Wittekind. Ch 22. Polyene dyes and fluorochromes, R.W. Horobin. Ch 23. Polymethines - 1. Cyanines, oxonols, benzimidazoles, indolenines and azamethines, R.W. Horobin. Ch 24. Polymethine dyes - 2. Styryls, thiazoles, coumarins and flavonoids, R.W. Horobin. Ch 25. Carbonyl dyes including indigoids, anthraquinones and naphthalimides, R.W. Horobin. Ch 26. Phthalocyanines, porphyrins and related azaannulenes, R.W. Horobin. Ch 27. Miscellaneous inorganic and organic substances used as biological stains, J.A. Kiernan and R.W. Horobin. Ch 28. Methods for testing biological stains, D.P. Penney and J.M. Powers with the assistance of C. Willis, M. Frank and C. Churukian.