Showcasing the recent progresses of the field, Cyclic Nucleotide Signaling covers the major tools and methodologies used in various areas of research. The majority of the chapters are protocol oriented, with the goal of providing clear directions for laboratory use. Students and investigators new to the field will find this book particularly informative, as will scientists already actively researching nucleotide signaling.
Table of Contents
Compartmentalization of cyclic nucleotide signaling. Analysis of EPAC-mediated cAMP functions in cell communication. Cyclic nucleotide analogs as pharmacological tools for studying signaling pathways. Discovery of small molecule EPAC specific modulators. Adenylyl cyclase and A kinase-anchoring proteins in cardiovascular physiology and pathology. Cyclic nucleotide recognition and selectivity. Real-time intracellular imaging of cGMP. Dissecting cyclic nucleotide signalosomes by chemical proteomics. Cyclic nucleotide analogs as chemical tools for interaction analyses. cAMP signaling in pancreatic ß cells. Quantitative high throughput cAMP assay for G-protein coupled receptors. Quantitative proteomics analysis of the cAMP signaling pathway. Network analysis of cyclic nucleotide signaling. Evolution and functional diversity of cyclic nucleotide binding domains. X-ray crystallographic analysis of cyclic nucleotide activated protein kinases. Dissect physiological functions of PKA isoforms using genetically modified mouse models. Assessing cyclic nucleotide binding domain allostery by NMR analysis. Fluorescence resonance energy transfer (FRET) imaging of cyclic nucleotide in live cells. Structural and functional analyses of exchange protein directly activated by cAMP. Structural analysis of PKA: strategies for therapeutic intervention. Monitoring cyclic nucleotide signaling using genetically encoded fluorescent reporters.