Genomic technologies provide the means of diagnosis and management of many human diseases. Without insights from cytogenetics, correct interpretation of modern high-throughput results is difficult, if not impossible. This book summarizes applications of cytogenetics and molecular cytogenetics for students, clinicians and researchers in genetics, genomics and diagnostics. The book combines the state-of-the-art knowledge and practical expertise from leading researchers and clinicians and provides a comprehensive overview of current medical and research applications of many of these technologies.
KEY FEATURES
• Provides clear summaries of fluorescence in situ hybridization technologies and others
• Comprehensively covers established and emerging methods
• Chapters from an international team of leading researchers
• Useful for students, researchers and clinicians
Series Preface
Scott O. Rogers
Preface
Acknowledgements
Editor
List of Contributors
Chapter 1 Cytogenetics and Chromosomics
Thomas Liehr
Chapter 2 Banding Cytogenetics
Hongyan Chai and Peining Li
Chapter 3 Generation of Microdissection-Derived Painting Probes from Single Copy Chromosomes
Svetlana Romanenko and Vladimir Trifonov
Chapter 4 FISH—An Overview
Thomas Liehr
Chapter 5 FISH—Microscopy and Evaluation
Ivan Y. Iourov
Chapter 6 FISH—in Routine Diagnostic Settings
Chenghua Cui and Peining Li
Chapter 7 FISH—in Leukemia Diagnostics
Roberto Matos, Mariana de Souza, Gerson Ferreira, Amanda Figueiredo, Marcelo Land and Maria Luiza Silva
Chapter 8 FISH—in Tissues
Thomas Liehr
Chapter 9 FISH—in Human Sperm and Infertility
Martina Rincic and Thomas Liehr
Chapter 10 FISH—in Spontaneously Aborted Products of Conception
Thomas Liehr
Chapter 11 FISH—Characterization of Chromosomal Alterations, Recombination, and Outcomes after Segregation
Ilda Patrícia Ribeiro, Eunice Matoso, Joana Barbosa Melo, Ana Jardim, Thomas Liehr and Isabel Marques Carreira
Chapter 12 Multicolor-FISH—Methods and Applications
Thomas Liehr
Chapter 13 FISH—Centromere- and Heterochromatin-Specific Multicolor Probe Sets
Thomas Liehr
Chapter 14 FISH—Detection of Individual Radio Sensitivity
Thomas Liehr
Chapter 15 FISH—Detection of CNVs
Tigran Harutyunyan, Anzhela Sargsyan and Rouben Aroutiounian
Chapter 16 FISH—Interphase Applications Including Detection of Chromosome Instability (CIN)
Ivan Y. Iourov, Svetlana G. Vorsanova and Yuri B. Yurov
Chapter 17 FISH—Determination of Telomere Length (Q-FISH/CO-FISH)
Gordana Joksić, Jelena Filipović Tričković and Ivana Joksić
Chapter 18 FISH—in Three Dimensions—3D-FISH
Thomas Liehr
Chapter 19 FISH—On Fibers
Thomas Liehr
Chapter 20 FISH—and Single-Cell Gel Electrophoresis Assay (Comet Assay)
Galina Hovhannisyan, Tigran Harutyunyan and Rouben Aroutiounian
Chapter 21 Molecular Karyotyping
Ilda Patrícia Ribeiro, Luís Miguel Pires, Susana Isabel Ferreira, Mariana Val, Joana Barbosa Melo and Isabel Marques Carreira
Chapter 22 FISH—Mitochondrial DNA
Tigran Harutyunyan
Chapter 23 FISH—in Birds
Rafael Kretschmer, Michelly da Silva dos Santos, Ivanete de Oliveira Furo, Edivaldo Herculano Correa de Oliveira and Marcelo de Bello Cioffi
Chapter 24 FISH—in Fish Chromosomes
Francisco de M. C. Sassi, Gustavo A. Toma and Marcelo de Bello Cioffi
Chapter 25 FISH—and the Characterization of Synaptonemal Complex
Victor Spangenberg
Chapter 26 RNA-FISH—on Lampbrush Chromosomes: Visualization of Individual Transcription Units
Tatiana Kulikova and Alla Krasikova
Chapter 27 FISH—in Insect Chromosomes
Vladimir E. Gokhman and Valentina G. Kuznetsova
Chapter 28 FISH—in Plant Chromosomes
Susan Liedtke, Sarah Breitenbach and Tony Heitkam
Chapter 29 FISH—and CRISPR/CAS9
Thomas Liehr
Index
Biography
Thomas Liehr is working in human genetics since 1991. He is biologist by education and head of the molecular cytogenetic group in Jena, Germany. Research fields include clinical genetics, leukemia cytogenetics and interphase structure of human chromosomes. The results of his research are published in >10 books, ~100 book chapter, ~800 referred papers, and ~1000 abstracts. His particular expertise includes small supernumerary marker chromosomes (sSMC), chromosomal heteromorphisms and uniparental disomy – see ChromosOmics databases (http://cs-tl.de/DB.html). He is active member of the European Board of Medical Genetics and received multiple prices, two invited professorships and a Dr.h.c. as summarized at http://cs-tl.de/.
