DNA Liquid-Crystalline Dispersions and Nanoconstructions: 1st Edition (Paperback) book cover

DNA Liquid-Crystalline Dispersions and Nanoconstructions

1st Edition

By Yuri M. Yevdokimov, V.I. Salyanov, S.V. Semenov, S.G. Skuridin

CRC Press

304 pages | 19 Color Illus. | 128 B/W Illus.

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Description

The discovery of the spatial structure of the double-stranded DNA molecule is one of the greatest achievements of science. It would not be an exaggeration to say that the DNA double helix is a distinguished symbol of modern biology.

Divided into three parts, DNA Liquid-Crystalline Dispersions and Nanoconstructions covers the information presently available on the condensation of various forms of DNA and describes practical applications of the peculiar properties of the liquid-crystalline particles.

  • Part 1 describes the main methods used for condensation of linear high- and low-molecular mass DNA, including their complexes with polycations and circular DNA
  • Part 2 compares the state and reactivity of double-stranded nucleic acid molecules fixed spatially in the liquid-crystalline as well as the same molecules under intracellular conditions
  • Part 3 explains how the discovery of the fundamental principles underlying the formation of nucleic acid liquid-crystalline dispersion particles opens a gate for the operational use of these principles in the area of nanotechnology and biosensorics

With detailed coverage of DNA liquid crystals, this book provides an understanding of the information presently available on the condensation of various forms of DNA. Double-stranded nucleic acids, spatially organized in a liquid-crystalline structure, represent an important polyfunctional tool for molecular biology and nanobiotechnology. The possibility of programmed and controlled variations in the properties of these molecules and in the characteristics of their liquid-crystalline dispersions, provides wide options for the formation of biologically active three-dimensional structures with unique, widely applicable properties.

Table of Contents

THE LIQUID-CRYSTALLINE STATE OF DNA

The Condensed State of the High-Molecular-Mass Double-Stranded DNA

The DNA Condensation and Aggregation

Polyphosphates as a Simplified DNA Model

Models of High-Molecular-Mass DNA Condensation in Water–Polymeric Solutions

Grosberg Model of High-Molecular-Mass DNA Condensation

Liquid-Crystalline Phases of the Low-Molecular-Mass Double-Stranded DNA Molecules

Ordering of Low-Molecular-Mass Double-Stranded DNAs

Brief Concept of Types of Liquid-Crystalline Phases

Liquid-Crystalline Phases of Low-Molecular-Mass Double-Stranded DNA Molecules

Dispersions of Low-Molecular-Mass Double-Stranded DNA Molecules

Low-Molecular-Mass Double-Stranded DNA Dispersions in Water–Polymer Solutions

Formation of DNA Dispersions in PEG-Containing Solutions

Circular Dichroism of Nucleic Acid Dispersions

Circular Dichroism as a Method of Proof of Cholesteric Packing of Nucleic Acid Molecules in Dispersion Particles and Analysis of Their Properties

Effect of Different Factors on Formation and Properties of CLCD Particles

Parameter of Nucleic Acid Molecule Order in CLCD Particles

Polymorphism of Liquid-Crystalline Structures Formed by (DNA-Polycation) Complexes

Some Peculiarities of Interaction of DNA Molecules with Polycations

Specificity of Chitosan Binding to DNA

Formation of Dispersions of (DNA–Chitosan) Complexes

CD Spectra of Dispersions Formed by (DNA–Chitosan) Complexes

X-Ray Parameters of Phases Formed by (DNA–Chitosan) Complexes

Dependence of Efficiency of CLCD Formation by (DNA–Chitosan) Complexes on Various Factors

Peculiarities of Interaction of Chitosan Molecules with Nucleic Acids

Attempt at a Theoretical Description of Interactions Occurring in the (DNA–Chitosan) Complexes and Resulting in the Formation of Liquid-Crystalline Dispersions with Different Optical Properties

Liquid-Crystalline State of DNA Circular Molecules

Phase Exclusion of Circular Molecules of Nucleic Acids

Formation of Dispersions From Circular Superhelical DNA

CD Spectra of Circular Superhelical DNA Dispersions Under Conditions That Modify Parameters of Their Secondary Structure

Packing Density and Rearrangement of the Spatial Structure of Superhelical DNA Molecules in LCD Particles

Topological Forms and Rearrangement of the Spatial Organization of Superhelical DNA Molecules in LCD Particles

DNA LIQUID-CRYSTALLINE FORMS AND THEIR BIOLOGICAL ACTIVITY

Liquid-Crystalline State of DNA in Biological Objects

DNA and Biological Objects

DNA Reactions under Conditions Causing Liquid-Crystalline Dispersions

Molecular Crowding

Condensation of DNA under the Effect of Chitosan in Conditions Causing Molecular Crowding

Activity of Nucleolytic Enzymes Under Conditions of Molecular Crowding

Activity of Proteolytic Enzymes under Conditions of Molecular Crowding

DNA LIQUID-CRYSTALLINE DISPERSIONS IN NANOTECHNOLOGY AND BIOSENSORICS

Nanoconstructions Based on Nucleic Acid Molecules

The General Concept of Nanotechnology

Biological Molecules as a Background for Nanodesign

Two Strategies of Nanodesign Based on NA Molecules

Biosensors Based on Nucleic Acids

General Concept of Construction and Operation of Biosensors

Double-Stranded DNA Molecule as Polyfunctional Biosensing Unit

Content and Principle of Operation of an Optical Biosensor Based on DNA Liquid-Crystalline Dispersions

DNA CLCD Particles as Sensing Units

Sandwich-Type Biosensing Units Based on (DNA–Polycation) Liquid-Crystalline Dispersions

DNA Nanoconstruction as a Sensing Unit (New Type of Biodetectors)

Hydrogels Containing DNA NaCs as New "Film-Type Biodetectors

Index

About the Authors

Yevdokimov Yuri Mikhailovich - Dr. Sci. (Chemistry), Professor: Head of the Condensed State of Nucleic Acids laboratory at Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences.

Interests: chemistry of natural and physiologically relevant compounds (especially, nucleic acids), lyotropic liquid crystals of biopolymers, biosensors, and nanotechnology of nucleic acids.

Salyanov Victor Ivanovich - Ph.D. (Chemistry): Senior research scientist of the Condensed State of Nucleic Acids laboratory at Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences.

Interests: chemistry of natural and physiologically relevant compounds, lyotropic liquid crystals of nucleic acids, and nanotechnology of nucleic acids.

Semenov Sergey Victorovich - Ph.D. (Physics): Senior research scientist at the Russian Research Center’s Kurchatov Institute

Interests: physics of liquid crystals, elementary particles physics and interaction of radiation with matter

Skuridin Sergey Gennadievich - Dr. Sci. (Molecular Biology): Leading research scientist of the Condensed State of Nucleic Acids laboratory at Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences.

Interests: biochemistry of natural and physiologically relevant compounds, lyotropic liquid crystals of nucleic acids, biosensors, and nanotechnology of nucleic acids.

About the Series

Liquid Crystals Book Series

The Liquid Crystals Book Series publishes authoritative accounts of all aspects of the field, ranging from the basic fundamentals to the forefront of research, from the physics of liquid crystals to their chemical and biological properties, and from their self-assembling structures to their applications in devices. The series provides readers new to liquid crystals with a firm grounding in the subject, while offering experienced scientists and liquid crystallographers an indispensable resource to enhance their own research.

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
SCI010000
SCIENCE / Biotechnology
SCI013000
SCIENCE / Chemistry / General
SCI013060
SCIENCE / Chemistry / Industrial & Technical
TEC021000
TECHNOLOGY & ENGINEERING / Material Science