220 Pages
by
Garland Science
224 Pages
by
Garland Science
Also available as eBook on:
The technique of DNA Sequencing lies at the heart of modern molecular biology. Since current methods were first introduced, sequence databases have grown exponentially, and are now an indispensable research tool. This up-to-date, practical guide is unique in covering all aspects of the methodology of DNA sequencing, as well as sequence analysis. It describes the basic methods (both manual and... Read more
What is DNA Sequencing? Chemical degradation (Maxam and Gilbert) method. Chain termination (Sanger dideoxy) method. Instrumentation and reagents. Template preparation. Gel electrophoresis. Non-radioactive methods. Troubleshooting. Confirmatory sequencing. Sequencing PCR products. Strategies for new sequence determination. Introduction to Bioinformatics and the Internet. Sequence databases. Sequence alignment and database searches. Sequencing projects and Contig analysis. Protein function prediction. Protein structure prediction. Appendices.
Biography
Dr Luke Alphey
