1st Edition

Dengue Diagnostics The Right Test at the Right Time for the Right Group

Edited By Shamala Devi Sekaran Copyright 2024
    116 Pages 12 Color Illustrations
    by Jenny Stanford Publishing

    116 Pages 12 Color Illustrations
    by Jenny Stanford Publishing

    Diagnostics plays a vital role in identifying infectious diseases that have the potential to become an epidemic, such as dengue. Good diagnostics enables identifying the cause of an outbreak and assessing interventions for a better impact. The dengue virus is a member of the family of flaviviruses that cross-reacts serologically with all members of its family. The presenting symptoms do not allow definitive diagnosis because they can be of malaria, dengue, Zika, chikungunya, or one of a host of other possibilities. In children, efficient and accurate dengue diagnostics is very important for the early confirmation of dengue because of its quick progress to severe dengue. Dengue diagnostic assays are wide-ranging—from being a reliable one that is time-consuming and expensive to rapid test kits that substantially vary in their accuracy. Therefore, it is important to know which test is to be used at what time, considering whether the population is in an endemic area, as well as how and when to use these tests, be it for the virus or for its genome, antigens, or antibodies. An ideal diagnostic test is one that can pick the virus early enough, is rapid and easy to perform, and affordable by all communities but such a test is yet not available. This book deals with most of the methods that have been used or developed for diagnosing dengue. It addresses the timeline for the evolution of the virus in the body, the body’s response from the onset of fever, and the role of diagnostics as time progresses. It covers most methods, detailing selected protocols, as well as compares them and assesses the time point at which they are useful. The book will be helpful in determining the right test, at the right time, for the right population.

    1. Introduction to Dengue and Issues with Current Diagnostics

    Shamala Devi Sekaran, Rishya Manikam, and Gaythri Thergerajan

    2. Traditional and Current Diagnostics

    Wang Seok Mui and Chandramathi Samudi Raju

    3. Newer Diagnostics for Dengue Disease

    Crystale Lim Siew Ying, Nur Haliza Hassan, and Shamala Devi Sekaran

    4. Search of Newer Markers for Dengue Disease

    Yong Yean Kong, Tan Hong Yien, Shamala Devi Sekaran, and Rishya Manikam

    5. Research and Recommendations

    Shamala Devi Sekaran


    Shamala Devi Sekaran is a professor in the Faculty of Applied Sciences at UCSI University Kuala Lumpur Campus, Malaysia. She teaches immunology and microbiology to undergraduates and postgraduates of medical, dental, and biomedical sciences. She obtained her PhD in 1986 on cell-mediated immune responses to dengue in mice. She is a fellow of the Academy of Sciences, Malaysia, since 2017 and fellow of the Royal Society of Pathologists, London, since 2019. Administratively, she was the director of the WHO collaborating center from 2003 to 2007; a member of the faculty committee for postgraduate degrees and viva voces, promotions, and counselling; coordinator for the MBBS, dental and biomedical courses; and head of the virology diagnostic laboratory. Her expertise is in research on infectious diseases, focusing on dengue and the development and evaluation of diagnostics, endothelial dysfunction mechanisms, and antivirals. She has received multiple awards for the kits developed, owns more than 20 patents (out of which 3 are commercialized), has published more than 250 research papers, and has an h-index of 44 with more than 6000 citations. Currently, she is investigating the effects of diabetes and obesity on severe dengue and attempting to develop serological assays to differentiate flaviviruses.