Electrochemical DNA Biosensors

Preface
Terminology Related to Electrochemical DNA-Based Biosensors Jan Labuda
Introduction
Detection Features of DNA-Based Biosensors
Detection of Specific DNA Interactions
Conclusions
Electrochemical Aptamer-Based Biosensors S. Centi, S. Tombelli, and M. Mascini
Introduction
Electrochemical Detection Strategies Based on Labeling
Electrochemical Aptasensors Based on a Sandwich Assay
Electrochemical Aptasensors Based on a Competitive Assay
Electrochemical Aptasensors Based on a Direct Assay 37
Electrochemical Metal Nanoparticle-Labeled Aptasensors 392.7 Electrochemical Aptasensors Based on Noncovalent Redox Species Label
Electrochemical Aptasensors Based on the Aptamer Conformational Changes
Electrochemical Aptasensors Based on Target-Induced Aptamer Displacement
Conclusions
Carbon-Polymer Bio-Nano-Composite Electrodes for Electrochemical Genosensing Mar´ıa Isabel Pividori and Salvador Alegret
Introduction
Composites Materials: Main Features and Classification
Carbon Composites
Electrochemical Genosensing Based on Graphite-Epoxy Composite
Final Remarks
Gold Nanoparticle-Based Electrochemical DNA Biosensors Mar´ıa Pedrero, Paloma Y´a ˜ nez-Sede ˜ no,
and Jos´e M. Pingarr´on
Introduction
Configurations Used for DNA Immobilization
Signal Transduction and Amplification Strategies
Conclusions and Outlook
Nanoparticle-Induced Catalysis for Electrochemical DNA Biosensors Marisa Maltez-da Costa, Alfredo de la Escosura-Mu˜ niz, and Arben Merkoc¸i
Introduction
Catalysis Induced by Gold Nanoparticles 1455.2.1 Electrocatalytic Activity of Gold Nanoparticle Labels on Silver Deposition
Catalysis Induced by Platinum and Palladium Nanoparticles
Catalysis Induced by Other Nanoparticles
Conclusions
Application of Field-Effect Transistors to Label-Free Electrical DNA Biosensor Arrays Peng Li, Piero Migliorato, and Pedro Estrela
Introduction
Field-Effect Transistors
Field-Effect DNA Sensing
Electrochemical Impedance Spectroscopy
Application of FETs on Biosensor Arrays
Conclusions
Electrochemical Detection of Basepair Mismatches in DNA Films Piotr Michal Diakowski, Mohtashim Shamsi,
and Heinz-Bernhard Kraatz
Introduction
Surface Immobilization
Detection Strategies
Conclusion
Electrochemical Detection of DNA Hybridization: Use of Latex to Construct Metal-Nanoparticle Labels Mithran Somasundrum andWerasak Surareungchai
Introduction
Synthesis of Metal Nanoparticles
Use of Metal Nanoparticles as Electrochemical Labels
Voltammetric Detection of Metal-Nanoparticle Labels
Latex as a Label Support
DNA Measurement
Areas for Further Research
Screen-Printed Electrodes for Electrochemical DNA Detection Graciela Mart´ınez-Paredes, Mar´ıa Bego ˜ na Gonz´ alez-Garc´ıa, and Agust´ın Costa-Garc´ıa
Introduction
Fabrication of Screen-Printed Electrodes
Genosensors on Screen-Printed Electrodes
Applications
Conclusion
Synthetic Polymers for Electrochemical DNA Biosensors Adriana Ferancov ´a and Katar´ına Ben´ıkov ´a
Introduction
Modification of Electrode Surface with Polymers
Polymer-Assisted DNA Immobilization
Application of Synthetic Polymers in DNA Biosensors
Conclusions
Electrochemical Transducer for Oligonucleotide Biosensor Based on the Elimination and Adsorptive Transfer Techniques Libuse Trnkova, Frantisek Jelen, and Mehmet Ozsoz
Introduction
Theoretical Fundamentals of Elimination Voltammetry with Linear Scan (EVLS)
EVLS Increasing the Transducer Potential Range
EVLS in Connection with Adsorptive Stripping Technique
EVLS of Nucleobases and Oligonucleotides in the Presence of Copper Ions
Conclusions
Electrochemical DNA Biosensors for Detection of Compound-DNA Interactions D. Ozkan-Ariksoysal, P. Kara, and M. Ozsoz
Introduction
The Structure of DNA
Natural Electronalytical Characterictics of DNA
Types of DNA Immobilization Methodologies onto Sensor Surfaces
DNA-Compound Interactions
Calculations About Compound-DNA Interactions
Conclusions
Electrochemical Nucleic Acid Biosensors Based on Hybridization Detection for Clinical Analysis P. Kara, D. Ariksoysal, and M. Ozsoz
Introduction
Biosensors
Electrochemical Nucleic Acid Biosensors
Conclusion
Nanomaterial-Based Electrochemical DNA Detection Ronen Polsky, Jason C. Harper, and Susan M. Brozik
Introduction
Nanoparticle-Based Electrochemical DNA Detection
Nanowires, Nanorods, and Nanofibers
DNA Detection Using Carbon Nanotubes
Conclusion
Electrochemical Genosensor Assay for the Detection of Bacteria on Screen-Printed Chips Chan Yean Yean, Lee Su Yin, and Manickam Ravichandran
Introduction
Methods for the Detection and Identification of Microorganism Utilizing Enzyme-Based Genosensors on Screen-Printed Chips
Advantages of the Enzyme-Based Electrochemical Genosensors in Detecting Bacteria on Screen-Printed Carbon Chips
Conclusions
Introduction to Molecular Biology Related to Electrochemical DNA-Based Biosensors Yalcin Erzurumlu and Petek Ballar
Introduction
Nucleic Acids
Deoxyribonucleic Acid
DNA in Electrochemical DNA-Based Biosensors
Nucleic Acid Variants Used in Electrochemical DNA-Based Biosensors
Index

"The marriage of natural and synthetic nanotechnology in electrochemical DNA sensors is a fascinating object of research. The reader gets an easy access to the complex matter by the well-written introductory chapter. This volume builds a bridge from molecular biology to the applications in medical diagnostics and microbiology."
—Prof. Frieder Scheller - Universität Potsdam, Germany

"This book is a very welcome contribution to the literature of electrochemical DNA biosensors. It offers extremely useful insights into this exciting and important field."
—Dr. Joseph Wang - University of California, San Diego, USA