1st Edition

Engineering Disease Resistance in Plants using CRISPR-Cas

By Zulqurnain Khan Copyright 2023
    168 Pages 14 B/W Illustrations
    by CRC Press

    CRISPR genome-editing technology presents opportunities to engineer disease resistance traits in plants and improve crop quality. Engineering Disease Resistance in Plants using CRISPR-Cas introduces readers to the basics of CRISPR-Cas and discusses its potential uses in various fields. The book focuses on methods of developing disease-resistant crops using CRISPR-Cas–mediated plant disease resistance modification.

    Comprehensively written, the author details all types and variants of the CRISPR toolkit. The book opens with information on the evolution of the CRISPR technology and follows a chronology of its development. Although the book concentrates on the use of CRISPR-Cas for disease resistance in plants, it also covers the technology's broader potential examining the history and development of other genome-editing tools.

    Key Features:

    • Investigates the regulatory, ethical, and societal considerations while designing experiments.
    • Discusses topics on disease development, control, and plant defense mechanisms.
    • Examines genome-editing tools including Zinc Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs).
    • Examines production technology to reduce bacterial, fungal, and viral diseases.
    • Provides information for users to discover ways to overcome the challenges associated with food security.


    This book is a valuable resource for researchers, scientists, and undergraduate and graduate students who wish to gain a comprehensive understanding of genome-editing methods.

    Chapter 01 History and Development of CRISPR/Cas: A Bacterial Defense System

    Chapter 02 Plant Defense and Disease Resistance Mechanisms

    Chapter 03 CRISPR/Cas: An Arsenal against Plant Diseases

    Chapter 04 Methods for Designing Disease-resistant Plants using CRISPR Technology

    Chapter 05 Developments in plant viral resistance using the CRISPR/Cas system

    Chapter 06 Utilizing CRISPR/ Cas System to Develop Resistance Against Bacterial Diseases

    Chapter 07 CRISPR/Cas 9 System to Combat Plant Fungal Infections

    Chapter 08 Bioethics and Risk Assessment of CRISPR Edited Crops


    Dr. Zulqurnain Khan is an Assistant Professor at the Institute of Plant Breeding and Biotechnology (IPBB), MNS University of Agriculture, Multan (MNSUAM), in Multan, Pakistan. Dr. Khan earned his PhD in Biotechnology in 2017 from the University of Agriculture, Faisalabad (UAF) in Pakistan. He is Pakistan's first PhD candidate, with a thesis on genome editing. He has been involved in genome editing since 2012. His study focuses on employing genome editing techniques (TALEs, TALENs, Cas9, dCas9, and multiplexed CRISPR/Cas9) in model plants and cotton to develop resistance to begomoviruses. He is also employing CRISPR/Cas9 technology to enhance cereal crop genetics for abiotic and biotic stress tolerance. He has edited two books on genome editing, one book on cotton breeding, and has authored over twenty book chapters. In addition, he has several review and research papers published in international peer-reviewed journals. Dr Khan was awarded an IRSIP scholarship by the Higher Education Commission (HEC) of Pakistan and worked as a visiting researcher at Professor Caixia Gao's group at the Institute of Genetics and Developmental Biology (IGDB), Chinese Academy of Sciences (CAS), in Beijing, China. Since 2018, Dr. Khan has been teaching several courses in biotechnology, breeding, molecular biology, genetics, and genome editing.