Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolution to accelerate the development of novel enzyme functionalities. This reference gathers the diverse perspectives of nearly 80 scientists from around the globe and surveys all leading rational and random approaches to the artificial evolution of enzymes. Citing more than 1500 notable works, it outlines assays for enzyme activity, stability, and specificity and a wide variety of site-directed, redesign, and evolutionary engineering methods.
Enzyme design: concepts for protein engineering, Martin Lehmann; sequence families and modular organization of carbohydrate-active enzymes, Bernard Henrissat, Pedro M. Coutinho, Emeline Deleury and Gideon Davies; analyzing three-dimensional structures of variant enzymes, Richard Bott; quantitative modelling of lipase enantioselectivity, Jurgen Pleiss; rational redesign of haloalkane dehalogenases guided by comparative binding energy analysis, Jiri Damborsky, Jan Kmunicek, Tomas Jedlicka, Santos Luengo, Federico Gago, Angel R. Ortiz and Rebecca C. Wade. Computer simulations: a tool for investigating the biological function of complex biological macromolecules, Gunther H. Peters; calculations of ionization equilibria in proteins, Andrey Karshikoff; Modelling and optimization of directed evolution protocols, Gregory L. Moore and Costas D. Maranas. Enzyme diversity generation - site-directed and redesign: rational redesign of enzymes, Jens Erik Nielsen; details in the reaction mechanism of chitinases, Vincent G.H. Eijsink, Gustav Kolstad, Sigrid Gaseidnes, Bjornar Synstad, Martin G. Peter, Jens E. Nielsen, David Komander, Douglas Houston and Daan M.F. van Aalten; kinetic evolution to the catalytic core of the bacterial phosphotriesterase, Frank M. Raushel; protein engineering of PQQ glucose dehydrogenase, Satoshi Igarashi and Koji Sode; the proline rule - a concept for engineering protein stability, Yiuzuru Suzuki; homing endonucleases - tools and targets for protein engineering, Alfred Pingoud, Ann-Josee Noel, Vera Pingoud and Shawn Steuer and Wolfgang Wende. Enzyme diversity generation - evolutionary methods: evolutionary methods for protein engineering, Huimin Zhao and Wenjuan Zha; directed evolution by random mutagenesis - a critical evaluation, Thorsten Eggert, Manfred T. Reetz and Karl-Erich Jaeger; enzyme engineering by phage display, Patrice Soumillion, Daniel Legendre and Jacques Fastrez; in vivo gene shuffling in yeast - a fast and easy method for directed evolution of enzymes, Jens Sigurd Okkels; effective DNA shuffling methods for enzyme evolution, Osamu Kagami, Sang-Ho Baik and Shigeaki Harayama; exploring the functional space of combinatorial mutant libraries for the directed evolution of novel enzyme activities, Bengt Mannervik, Lars O. Hansson and William G. Bardsley; modifying the character of an enzyme by producing chimeric enzymes - chimeric -glucosidases as an illustration, Kiyoshi Hayashi, Bong Jo Kim, Kshamata Goyal, Satya Singh, Jong-Deog Kim, Yeon-Kye Kim, Satoru Nirasawa and Motomitsu Kitaoka. Screening: assay systems for screening or selection of biocatalysts, Uwe T. Bornscheuer; screening of enzyme variants for thermostability, Shigenori Kanaya. (Part ).