1st Edition
Experimental Surgical Models in the Laboratory Rat
An All-Inclusive Guide to Surgical Techniques on Rats
The design of an adequate surgical model, like the choice of the animal model itself, is extremely important for obtaining reliable valuable data. Experimental Surgical Models in the Laboratory Rat summarizes a series of techniques that were applied in the Bone Biology Laboratories, School of Medicine, Rosario National University, Argentina.
Stopping just short of an exhaustive analysis of the techniques, this manual describes the basics of each surgical technique, the necessary materials, precautions to consider, the methodology to apply, and the possible results to be obtained in similar experiments.
CD-ROM: 250 Illustrations of Surgery Techniques with Step-by-Step Instruction
With succinct descriptions, color photographs, and easy-to-follow steps, the downloadable resources are a practical, full-color roadmap for performing various surgery techniques, such as catheterization, tracheostomy, intramuscular injection, subcutaneous injection, intraperitoneal injection, and oral intubation. This definitive work provides technical and practical data including volumes and concentrations of solutions, catheters used, equipment, and biological variables on the rat. Experimental Surgical Models in the Laboratory Rat is a complete reference for today’s biomedical sciences investigator.
Section I: Introduction
Bioethics and Animal Care, Lucas R. M. Brun and Verónica Di Loreto
Control of Stress and Distress, María F. Landoni
Management of Pain in Laboratory Animals, María F Landoni
Anesthesia and Analgesia, Verónica E. Di Loreto, Alfredo Rigalli, and Lucas F. de Candia
Euthanasia, Alfredo Rigalli
Antibiotics, Verónica Di Loreto and Alfredo Rigalli
Section II: General Procedures
Operating Theatre, Verónica Di Loreto and Alfredo Rigalli
Rat Identification, Verónica Di Loreto and Alfredo Rigalli
Antisepsis, Sterilization, and Asepsis, Diego Holotte,- Jésica Nipoti, Maria Julia Pretini, and Verónica Di Loreto
Suture, Aneley Traverso and Alfredo Rigalli
Substances Administration, Maela Lupo, Verónica Di Loreto, and Alfredo Rigalli
Samples, Veronica Di Loreto, Laura Pera, and Alfredo Rigalli
Section III: Catheterism and Cannulation
Catheterism of the Femoral Artery and Vein, Verónica Di Loreto and Alfredo Rigalli
Cardiac Catheterization, Cristina Lorenzo Carrión, Laura Krieger, Manuel Rodríguez, and Martín Donato
Cannulation of the Thoracic Duct, Gabriel A. Inchauspe and Alfredo Rigalli
Tracheostomy, Laura L. Pera and Alfredo Rigalli
Section IV: Gastrointestinal Tract
In Situ Isolation of the Stomach, Alfredo Rigalli
In Situ Isolation of the Intestinal Loop, María L. Brance, Lucas R.M. Brun, and Alfredo Rigalli
Intestinal Everted, Lucas R. M. Brun, María L. Brance, Lucas F. de Candia, and Alfredo Rigalli
In Situ Perfusion of the Small Intestine, Alfredo Rigalli
Section V: Pancreas
Experimental Models for the Study of Diabetes, Verónica Di Loreto and Alfredo Rigalli
Islets of Langerhans Isolation, Alfredo Rigalli
Incubation of Pancreatic Tissue Slices, Inés Menoyo and Alfredo Rigalli
In Situ Perfusion of The Pancreas, Inés Menoyo and Alfredo Rigalli
Experimental Pancreatitis, Diego Holotte, Jésica Nipoti, Maria J. Pretini, Verónica Di Loreto, and Alfredo Rigalli
Section VI: Liver
Hepatic Circulation Impairment, Gabriel A. Inchauspe and Alfredo Rigalli
Extrahepatic Cholestasis Model, Anabel Brandoni and Adriana M. Torres
Section VII: Ablation of Endocrine Glands
Adrenalectomy, Laura Pera and Alfredo Rigalli
Ovariectomy, Verónica Di Loreto, Laura Pera, and Alfredo Rigalli
Thyroparathyroidectomy, Verónica Di Loreto and Alfredo Rigalli
Parathyroid Glands, Maela Lupo and Alfredo Rigalli
Section VIII: Kidneys
Measurement Techniques of Renal Parameters, Verónica Di Loreto and Alfredo Rigalli
Kidney Isolation and Perfusion, Verónica Di Loreto and Alfredo Rigalli
Acute Renal Failure Models, Gisela Di Giusto and Adriana M. Torres
Renal Blood Flow Measurement, Gisela Di Giusto, Adriana M. Torres, and Alfredo Rigalli
Transport Studies in Plasma Membrane Vesicles Isolated from Renal Cortex, Gisela Di Giusto and Adriana M. Torres
Remnant Kidney Model, María V. Arcidiacono, L. A. Ramirez, V. Dalmau, and Alfredo Rigalli
Section IX: Circulatory System
Experimental Myocardial Infarction, Ignacio Seropian and Germán E. González
Experimental Arteriosclerosis, Anabel Brandoni and Adriana M. Torres
Isolated Heart: Langendorff Technique, Bruno Buchholz, Verónica D´Annunzio, and Ricardo J. Gelpi
Section X: Additional Techniques
Polyclonal Antibodies Preparation, Laura I. Pera, Lucas R. M. Brun, and Alfredo Rigalli
Histological Procedures, María L. Brance and Lucas R. M. Brun
Bones and Bone Tissue, Hilda Moreno, Mercedes Lombarte, and Verónica Di Loreto
Section XI: Miscellaneous
Equipment, Alfredo Rigalli
Solutions Preparation, Verónica Di Loreto and Alfredo Rigalli
Normal Values In Iim/Fm Rat Subline "M", Veronica Di Loreto and Alfredo Rigalli
Abbreviations
