1st Edition
Experiments in Cell and Molecular Biology A Laboratory Manual for Undergraduate Instruction or Concepts and Techniques for the Undergraduate Laboratory
Description, Prerequisites, Mission Statement
Learning Goals
Learning Objectives
General Laboratory Instructions
(A) Laboratory Safety Rules
(B) Procedures for Emergency Medical Care
(C) Guidelines for Keeping Laboratory Notebooks
(D)Guidelines for the Preparation of Laboratory Reports
(E) Citation Style Guide
(F) Pipetting
Laboratory No. 1: Bioinformatics: Self-guided Internet-based Exercise on Databases for the Storage and Data Mining
Laboratory No. 2: Biological Buffers I. Titration of the Amino Acid Glycine
Laboratory No. 3: Biological Buffers II. Preparation of a Phosphate Buffer
Laboratory No. 4: Determination of Protein Concentration using the Bradford Method
Laboratory No. 5: Enzyme Kinetics
Laboratory No. 6: Assay of Cytochrome c Oxidase in Potato Tuber Mitochondria
Laboratory No. 7: Molecular Evolution: Separation of Fish Proteins Using Polyacrylamide Gel Electrophoresis
Laboratory No. 8: Genetic Fingerprinting
Laboratory No. 9: Molecular Biology of Sickle-cell Anemia
Laboratory No. 10: Signal Transduction: Antagonistic effects of Gibberellin and Abscisic Acid on the Production of a-Amylase in Barley Seeds
Biography
Miguel Cervantes-Cervantes was born in Atotonilco el Alto, Jalisco, Mexico. He completed his B.Sc. in Biology and M.Sc. in Biochemistry at the National Polytechnic Institute, in Mexico City. He obtained a Ph.D. in Biochemistry from Rutgers University, New Brunswick, New Jersey, under the direction of Dr. Carl A. Price. Dr. Cervantes’ post-doctoral training included work at the Department of Biological Sciences of Rutgers University-Newark with Dr. Douglas Bush. Dr. Cervantes previously held a faculty position at the City University of New York, where his research focused on plant carotenoid biosynthesis. He currently works at the Department of Biological Sciences, Rutgers University-Newark, where he teaches biochemistry, microbiology, and genetics, among other subjects. Dr. Cervantes has taught the cell and molecular biology core course for biology majors and minors for many years. He has also taught a graduate course in college teaching. He has designed hands-on laboratory courses that emphasize experimental approaches to understanding biological processes. Out of the classroom and the laboratory, he enjoys drawing, painting, and traveling to explore cultures different from his own.
Born in Ho Chi Minh City, Vietnam, Dr. Tracy S. Tran earned her B.Sc. degree in Neuroscience, M.Sc. and Ph.D. degrees in Physiological Sciences and Molecular, Cellular and Integrative Physiology, respectively, from the University of California, Los Angeles (UCLA) with Dr. Patricia E. Phelps. Dr. Tran conducted her postdoctoral training in neuroscience at the Johns Hopkins University School of Medicine, with Drs. Alex Kolodkin and David Ginty. Currently, Dr. Tran is a Professor in the Department of Biological Sciences at Rutgers University, NJ, USA, and the Director of the Undergraduate Program in Biology. Dr. Tran’s laboratory investigates the molecular and cellular mechanisms that control neuronal morphogenesis and wiring in the mammalian nervous system. Her research aims to elucidate the molecular signaling involved in how neurons assume their diverse morphologies, the axons and dendrites, which enables the assembly of neural circuits required for complex behavior and cognitive function. When not doing research, Dr. Tran collaborates with colleagues to develop creative laboratory manuals to make learning about science and research fun for students. Finally, in her spare time, she enjoys traveling to visit her research collaborators’ labs or going on adventures to meet new people and try new things.
