Extracellular Vesicles in Health and Disease

SECTION 1 OVERVIEW
Overview of extracellular vesicles in health and disease – Rienk Nieuwland, Edwin van der Pol and Augueste Sturk
The Biogenesis of Exosomes – Michiel Pegtel
Extracellular vesicle-mediated epigenetic reprogramming of cells G.Camussi, F. Collino, M.C. Deregibus

SECTION 2 TECHNOLOGIES FOR MEASUREMENT OF EXTRACELLULAR VESICLES
An overview of novel and conventional methods to detect extracellular vesicles. E. van der Pol, T.G. van Leeuwen, R. Nieuwland
Standardization and Pre-analytical Variables Romaric Lacroix, Coralie Judicone, Stéphane Robert, Laurent Arnaud, Florence Sabatier and Françoise Dignat-George
Procoagulant Assays Barry Woodhams
Capture Based Assays for Extracellular Vesicles within the Blood Jean Amiral
Flow Cytometry Romaric Lacroix, Stephane Robert, Florence Sabatier and Francoise Dignat-George
Atomic force microscopy measurement of extracellular vesicles derived from plasma Yuana Yuana and Susanne Osanto
Light scattering methods to characterize extracellular vesicles Don A Gabriel MD PhD, Sam Glover, Regina Bowling and Nigel Key
Nanoparticle Tracking Analysis Rebecca Dragovic and Chris Gardiner
Microvesicle and exosome proteomic analysis Extracellular vesicle proteomic analysis Andrés F. Parguiña and Ángel García

SECTION 3 CLINICAL PERSPECTIVES
Microvesicles and exosomes in Cancer Rebecca Karp and Jeffrey Zwicker
Extracellular Vesicles and tissue factor Pierre-Emmanuel Rautou and Nigel Mackman
Extracellular vesicles in normal pregnancy and pre-eclampsia Ian Sargent, Rebecca Dragovic, Dionne Tannetta and Christopher Redman
Extracellular vesicles in Cardiovascular Disease Anne-Clémence Vion, and Chantal M. Boulanger
Extracellular vesicles in Immunology Els J. van der Vlist, Esther N.M Nolte’t Hoen and Marca H.M. Wauben

Biography

Paul Harrison is the Healing Foundation senior lecturer within the School of Immunity and Infection at the University of Birmingham, UK. He is a former president of the British Society of Haemostasis and Thrombosis. He has published over 120 papers and book chapters/reviews in his field. In 2010, Paul was featured as one of the top 100 healthcare scientists in the United Kingdom by the Department of Health. He is also an associate editor of the Journal of Extracellular Vesicles and a member of the editorial board of Platelets and the Journal of Thrombosis and Haemostasis.

Chris Gardiner is scientific secretary of the British Society for Haemostasis and Thrombosis and a board member of both the International Society of Extracellular Vesicles and International Society of Laboratory Hematology. He serves on the editorial board of the Journal of Extracellular Vesicles and the International Journal of Thrombosis and Haemostasis. He has vast experience on the measurement and characterization of extracellular vesicles in the early detection of human disease.

Ian L. Sargent is professor of reproductive science in the Nuffield Department of Obstetrics and Gynaecology, University of Oxford, UK. He has worked in the field of reproductive immunology and trophoblast biology for more than 30 years and has over 150 publications on a wide range of topics including pre-eclampsia, feto-maternal cell traffic, HLA-G, and early human embryo development and implantation. A major focus of his work has been the isolation and characterization of placental extracellular vesicles and their role in the pathogenesis of pre-eclampsia.