Jenny Stanford Publishing
482 pages | 20 Color Illus. | 41 B/W Illus.
Interest in the role of extracellular vesicles (microvesicles and exosomes) is expanding rapidly. It is now apparent that far from being merely cellular debris, these vesicles play a key role in cell-to-cell communication and signaling. Moreover, they are significantly elevated in a number of diseases. This raises the question of their direct role in pathogenesis as well as their possible use as biomarkers.
This book stems from the first international meeting on "Microvesicles and Nanovesicles in Health and Disease" held at Magdalen College, Oxford, in 2010. The purpose of the meeting was to bring together, for the first time, a range of experts from around the world to discuss the latest advances in this field. Key to the study of these vesicles is the availability of methodologies for their measurement in biological fluids. A major section of the meeting focused on a range of exciting new technologies which have been developed for this purpose. The presentations at this meeting form the basis of this book, which will appeal to basic scientists, clinicians, and those developing technology for the measurement of extracellular vesicles.
SECTION 1 OVERVIEW
Overview of extracellular vesicles in health and disease – Rienk Nieuwland, Edwin van der Pol and Augueste Sturk
The Biogenesis of Exosomes – Michiel Pegtel
Extracellular vesicle-mediated epigenetic reprogramming of cells G.Camussi, F. Collino, M.C. Deregibus
SECTION 2 TECHNOLOGIES FOR MEASUREMENT OF EXTRACELLULAR VESICLES
An overview of novel and conventional methods to detect extracellular vesicles. E. van der Pol, T.G. van Leeuwen, R. Nieuwland
Standardization and Pre-analytical Variables Romaric Lacroix, Coralie Judicone, Stéphane Robert, Laurent Arnaud, Florence Sabatier and Françoise Dignat-George
Procoagulant Assays Barry Woodhams
Capture Based Assays for Extracellular Vesicles within the Blood Jean Amiral
Flow Cytometry Romaric Lacroix, Stephane Robert, Florence Sabatier and Francoise Dignat-George
Atomic force microscopy measurement of extracellular vesicles derived from plasma Yuana Yuana and Susanne Osanto
Light scattering methods to characterize extracellular vesicles Don A Gabriel MD PhD, Sam Glover, Regina Bowling and Nigel Key
Nanoparticle Tracking Analysis Rebecca Dragovic and Chris Gardiner
Microvesicle and exosome proteomic analysis Extracellular vesicle proteomic analysis Andrés F. Parguiña and Ángel García
SECTION 3 CLINICAL PERSPECTIVES
Microvesicles and exosomes in Cancer Rebecca Karp and Jeffrey Zwicker
Extracellular Vesicles and tissue factor Pierre-Emmanuel Rautou and Nigel Mackman
Extracellular vesicles in normal pregnancy and pre-eclampsia Ian Sargent, Rebecca Dragovic, Dionne Tannetta and Christopher Redman
Extracellular vesicles in Cardiovascular Disease Anne-Clémence Vion, and Chantal M. Boulanger
Extracellular vesicles in Immunology Els J. van der Vlist, Esther N.M Nolte’t Hoen and Marca H.M. Wauben