Fluorescence Imaging and Biological Quantification
This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology.
Confocal microscopy in the life sciences; Imaging flow cytometry for quantification of cellular parameters; Live-cell imaging: Seeing is believing; Atomic force microscopy: A tool for imaging, manipulating biomolecules, and quantifying single-molecule interactions in biological systems; Confocal Raman microscopy: Imaging the chemistry; Tracking cancer: In vivo imaging techniques; Quantum dots: Concepts, imaging, and therapeutic applications in cancer; Selecting the appropriate in situ proximity ligation assay protocol; Cell detection and joint shape tracking using local image filters; Studying subcellular signaling events in living microglial cells using fluorescence resonance energy transfer-based nanosensors; Membrane trafficking under the microscope, what new imaging technologies have brought to light; Illuminating the cycle of life; Methods for the visualization of circadian rhythms: From molecules to organisms; Measurement of lysosomal ion homeostasis by fluorescence microscopy; Capturing quantitative features of protein expression from in situ fluorescence microscopic images of cancer cell populations; Cancer cell invadopodia: Visualization and quantification tools.
"Microscopy techniques have brought significant improvements and novel implementations to several fields in the past few years. This compendium offers a fresh review of several techniques (fluorescence confocal, Raman and AFM) and their applications to life sciences; it will be useful to the experienced researcher as well as to the student approaching the use and applications of microscopy techniques."
— Beniamino Barbieri, ISS, Champaign, Illinois, USA