This book covers the fundamental principles of fluorescence and their application to fluorescence microscopy, and presents applications to immunofluorescence, in situ hybridization, and photomicrography. It provides troubleshooting guidance to guide the user through commonly encountered problems.
1. Fundamentals of fluorescence 2. Fluorescence microscopy 3. Practical fluorescence microscopy 4. Applications of fluorescence microscopy 5. Quantitative fluorescence microscopy 6. Digitized video microscopy 7. Single and multiphoton microscopy 8. Fluorescence photomicroscopy