Fluorescence-based sensing is a significant technique used in prominent fields such as fluorescence-activated cell sorting, DNA sequencing, high-throughput screening, and clinical diagnostics. Fluorescence Sensors and Biosensors emphasizes the most recent developments and emerging technologies with the broadest impacts.
The text begins with the development of aptamers (oligoribonucleotides) and biorecognition techniques based on periplasmic binding proteins. The following chapters review the molecular beacon approach for DNA recognition, describe resonance energy transfer (FRET) in sensing, and present the use of carbonic anhydrase recognition platform for metal ion determination and imaging. The book explores the advantages of fluorophores, fluorescent labels, sensor and assay construction, metal-enhanced fluorescence, phosphorescent labels, and lab-on-a-chip applications. It also describes new anion-selective fluorescent probes used as analytes in clinical determinations.
The final chapters highlight the application of fluorescence sensing technology to several practical problems, such as the development of planar waveguide biosensors for clinical diagnostics and the adaptation of fluorescence-based sensing approaches for biochemical production by fermentation. The book also discusses the measurement of analytes, such as free zinc ions, at ultratrace levels in biological specimens. Written by internationally renowned authors in their fields, Fluorescence Sensors and Biosensors provides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology, analytical chemistry, and biomedicine.
Table of Contents
Introduction; Prospects for the De Novo Design of Nucleic Acid Biosensors; Biosensors Based on Periplasmic Binding Proteins; Molecular Beacon DNA Probes Based on Fluorescence Biosensing; Fluorescence Resonance Energy Transfer-Based Sensors for Bioanalysis; Carbonic Anhydrase-Based Biosensing of Metal Ions: Issues and Future Prospects; Metal-Enhanced Fluorescence Sensing; Subpicomolar Assays of Antibodies and DNA Using Phosphorescence Labels; Development of Fluorescent Dipyrrolylquinoxaline-Based Anion Sensors;Lab-on-a-Chip and Fluorescence Sensing on the Microscale; The Array Biosensors; Planar Waveguide Biosensors for Point-of-Care Clinical and Molecular Diagnostics; Fluorescence-Based Sensors for Bioprocess Monitoring; Practical Aspects of Fluorescence Analysis of Free Zinc Ion in Biological Systems: pZn for the Biologist;