1st Edition

Forensic Analysis of Biological Evidence A Laboratory Guide for Serological and DNA Typing

By J. Thomas McClintock Copyright 2014
    176 Pages 43 B/W Illustrations
    by CRC Press

    176 Pages
    by CRC Press

    A powerful tool in the identification of individuals, DNA typing has revolutionized criminal and paternity investigations. Widespread analysis is now conducted by public and private laboratories in the United States and abroad. Focusing on the basic techniques used in forensic DNA laboratories, Forensic Analysis of Biological Evidence: A Laboratory Guide for Serological and DNA Typing introduces readers to the science of serological analysis and DNA typing methods and provides a thorough background of the molecular techniques used to determine an individual’s identity or parental lineage.

    Originally published as Forensic DNA Analysis: A Laboratory Manual, this revised work offers updated exercises and protocols for all kinds of DNA and serological analyses with delineated objectives, step-by-step procedures, and required laboratory supplies. Each exercise in this manual:

    • Provides an overview of forensic DNA analysis
    • Explains the sources or types of biological material used in a particular DNA analysis
    • Supplies the background principles and practical methodology for specific serological analysis and DNA typing techniques
    • Simulates human forensic testing and can also be used to simulate a wide range of applications for genetic analysis

    The book contains an extensive glossary to make readers familiar with terminology used in the forensic analysis of biological evidence, as well as basic terms used in molecular biology. Those who master the material in this volume will understand the methodology of the investigation in DNA typing, develop an understanding of the scientific principles involved in serology and DNA analysis, and succeed in analyzing and interpreting the data generated in each exercise with clarity and confidence.

    An Overview of Forensic DNA Analysis
    Types of Biological Specimens
    Exercise 1: Evidence Examination Using an Alternate Light Source
    Forensic Serology
    Exercise 2: Detection of Saliva
    Exercise 3: Detection of Blood
    Exercise 4: Detection and Identification of Semen
    Exercise 5: Detection and Identification of Urine
    Exercise 6 - DNA Extraction
    Exercise 7: Concentration of Extracted DNA
    Exercise 8: Microcon Concentration and Purification of Extracted DNA
    Exercise 9 - Assessing the Quality and Quantity of Isolated DNA
    The Polymerase Chain Reaction (PCR)
    Exercise 10: Short Tandem Repeat (STR) Analysis
    Exercise 11: Using Short Tandem Repeat (STR) Analysis to Determine Paternity (A Case Study)
    Exercise 12: Polymerase Chain Reaction (PCR)-Based Tests: Y-Chromosome Short Tandem Repeat (Y-STR) Analysis (A Case Study)
    Exercise 13: Mitochondrial DNA (mtDNA) Analysis
    Exercise 14: Assessment of DNA Typing Data
    Single Nucleotide Polymorphisms (SNPs) for Human Identification: An Emerging Forensic Tool
    References and Suggested Readings
    Appendix A: Composition of Buffers and Solutions
    Appendix B: Using a Micropipet
    Appendix C: Glossary


    J. Thomas McClintock