1st Edition
Forensic Applications of High Performance Liquid Chromatography
Introduction to HPLC
Introduction
The History of Chromatography
Basic Instrumentation
Further Reading
Basic Principles of HPLC
Theory of Chromatography
Chemical Bonding and Polarity
Mobile Phase Preparation, the Use of Buffers, and Sample Preparation
Mobile Phase Preparation
Buffers
Sample and Standard Preparation
Modes of Separation
Introduction
Reverse Phase HPLC
Ion Exchange
Normal Phase HPLC
Detection Systems
Introduction
Theory
UV/Vis Detectors versus Diode Array Detectors
Electrochemical Detectors
Mass Spectrometry
Method Development in Reversed Phase HPLC
Introduction
Sample Composition
Which Column Will Affect the Separation?
Choosing a Mobile Phase
Automated Method Development
Method Development Strategy
Method Development Worked Example
System Suitability
Introduction
System Suitability
System Performance
Chromatographic Suitability
Qualification, Validation, and Verification
Introduction
Valid Analytical Measurement
Estimation of Uncertainty of Measurement, Sources of Error, and Statistical Evaluation
Instrument Qualification
Method Validation
Method Verification
Quality Systems
Introduction
What Is Quality?
Introduction to Quality Assurance
How Do We Ensure Quality in Our Laboratories?
A Quality Management System
Accreditation
Auditing
Troubleshooting HPLC Systems
Introduction
Troubleshooting
Tool Kit and Spare Parts
Preventative Maintenance
Column Care
Forensic Applications of HPLC
Introduction
Drug Analysis
Toxicological Analysis
Colour Analysis
Analysis of Explosives
Food and the Environment
Glossary
Index
Biography
Shirley Bayne, Michelle Carlin
I showed this book to many of my students and asked them to read it and they told me that they could understand the concepts of HPLC properly for the first time from this book. Even a number of my own concepts were clarified a great deal. … This is the first book to the best of my knowledge specifically dealing with the forensic applications of HPLC, and a very good one at that. Highly recommended for all forensic scientists and chemists, especially those who are working in separation science.
— Anil Aggrawal writing in Anil Aggrawal's Internet Journal of Forensic Medicine and Toxicology
"… easy to read and lively enough to engage students and keep their interest."
— C. F. Poole, Wayne State University, in Chromatographia
"The book chapters maintain a balance between theoretical considerations and examples and practical solutions. … designed as a textbook for university students studying analytical chemistry, applied chemistry, forensic chemistry, or other courses with an element of HPLC within the course curriculum, this volume is an invaluable guide for those in the early stages of their forensic analysis careers."
—International Journal of Environmental Analytical Chemistry
