1st Edition

From Proteins to Proteomics Basic Concepts, Techniques, and Applications

By Sanjeeva Srivastava Copyright 2023
    272 Pages 50 Color & 153 B/W Illustrations
    by CRC Press

    272 Pages 50 Color & 153 B/W Illustrations
    by CRC Press

    272 Pages 50 Color & 153 B/W Illustrations
    by CRC Press

    Proteomics aims to study all the proteins of human and other living systems, as well as their properties to provide an integrated view of cellular processes. The study of proteomics involves the application of rapidly evolving high-throughput technologies and new platforms that are coming forward regularly, providing versatile novel tools for biomedical and pharmaceutical applications.

    This book provides a detailed understanding of the basics of proteins and proteomics, gel based-proteomics techniques, basics of mass spectrometry and quantitative proteomics, interactomics: basics and applications, and advancements in proteomics. It also covers basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics.

    The text highlights the application and challenges of various high-throughput integrated proteomics technologies capable of fast and accurate screening of thousands of biomolecules, which are found to be very effective for studying disease pathobiology and identification of next-generation biomarkers and potential drug/vaccine targets; and are therefore considered valuable tools for multidisciplinary research.


    • Basics of proteins and proteomics techniques
    • In-depth understanding of mass spectrometry and quantitative proteomics
    • An overview of interactomics and its application for translational research
    • Advancement in the field of proteomics and challenges in clinical applications

    We hope the knowledge gained from reading this book will intrigue and motivate young minds to explore future opportunities in the constantly evolving field of proteomics.

    Module 1: Basics of proteins and proteomics

    Basics of amino acids and proteins

    Protein chemistry to proteomics

    Module 2: Gel-based proteomics techniques

    Gel-based proteomics

    2-d difference in gel electrophoresis (2d-dige)

    Gel-based proteomic data analysis

    Module 3: Basics of mass-spectrometry and quantitative proteomics

    Introduction to mass spectrometry

    Hybrid mass spectrometry configurations

    Tandem mass spectrometry for protein identification

    In vitro quantitative proteomics using itraq

    In vivo quantitative proteomics using silac

    Module 4: Interactomics: basics and application

    Introduction to interactomics

    Antigen and antibody microarrays

    Cell-free expression based protein microarrays

    Nucleic acid programmable protein arrays

    Label-free proteomics

    Surface plasmon resonance

    Surface plasmon resonance imaging

    Protein interaction analysis using spr and spri

    Module 5: Advancement in proteomics

    Proteomics for translational research

    Future of proteomics for clinical applications

    Challenges in clinical proteomics


    Prof. Sanjeeva Srivastava is a professor and the group leader of the Proteomics Laboratory at the Department of Biosciences and Bioengineering at the Indian Institute of Technology, Bombay. High-throughput proteomics, protein microarrays, and mass spectrometry are among his specialties. He has implemented ground-breaking AI-driven data analytics on big biological datasets. He has been at the forefront of biomedical research based on big data. His group's current research focuses on the development of clinical biomarkers for infectious diseases and malignancies. His group also pioneered therapeutic target identification efforts and decoded protein interaction networks in human illnesses like gliomas and contagious diseases like COVID-19 and malaria. His group has developed reliable diagnostic biomarkers and described the pathophysiology of severe malaria (falciparum and vivax) and COVID-19, especially the underlying mechanisms that lead to the development of severe sequelae.

    Dr. Srivastava is an active contributor to global proteomics research and development. He serves on the Executive Committee of Human Proteome Organization (HUPO) and Proteomics Society, India (PSI). He has more than 125 publications from his work as an independent researcher at IIT Bombay. To date, he has filed 15 patents that include biomarkers for various types of cancers, infectious diseases like malaria, leptospirosis, COVID-19, and method improvement in uncharted territories such as fish and plant proteomics.