Functional Protein Microarrays in Drug Discovery  book cover
1st Edition

Functional Protein Microarrays in Drug Discovery

Edited By

Paul F. Predki

ISBN 9780849398094
Published March 30, 2007 by CRC Press
480 Pages 148 B/W Illustrations

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Book Description

As central actors in most biological functions, proteins are the subject of intense study. This has driven the development of increasingly sophisticated approaches for the study of proteins, which has extended to proteomic level methodologies. Yet currently no book has addressed all aspects of functional microarrays in a coherent and integrated fashion. Until now. Meeting the need for comprehensive and authoritative information, Functional Protein Microarrays in Drug Discovery provides an up-to-date overview of the field and the background required to actually design and develop arrays or perform and analyze array experiments.

The book is divided into five main sections that address critical aspects of the field. The first focuses on the generation of functional protein content, the first and perhaps most challenging aspect of protein microarrays. The second section describes ‘standard’ and state-of-the-art fabrication methods, focusing on issues of particular significance to functional protein microarrays. Similarly, the third section reviews current and next generation approaches to assay detection. The fourth and largest section, dedicated to applications, spans the breadth of published applications, from biomolecular interaction discovery and characterization to humoral response biomarker profiling, enzyme substrate identification and drug discovery. The final section addresses fundamental computational issues including image and data analysis as well as data visualization.

While the field’s early successes have set the stage for rapid growth, it is not without challenges. Indeed, challenges are to be expected in a fast-moving, interdisciplinary endeavor such as this, where molecular biology, protein chemistry, bioinformatics, engineering, and physical sciences intersect. As the first integrated reference for functional protein microarrays, this book helps you not only meet the challenges but also excel in your research.

X. Y. Zhu, one of the contributors to this text, was recently featured in an article in Austin's The Statesman.

Daniel E. Levy, editor of the Drug Discovery Series, is the founder of DEL BioPharma, a consulting service for drug discovery programs. He also maintains a blog that explores organic chemistry.

Table of Contents

Functional Protein Content for Microarrays
High-Throughput Gene Cloning Using the Gateway®
Technology, S.N. Peterson, P. Burr, G. Tsegaye, and P. Venepally
Protein Expression for MicroArrays, H.H. Yim, T.G. Chappell, and S.H. Harwood
Emerging Trend: Cell-Free Protein Expression, F. Katzen and W. Kudlicki
Fabrication of Functional Protein Microarrays
The Critical Role of Surface Chemistry in Protein Microarrays, A. Guo and X.-Y. Zhu
Fabrication of Sol-Gel-Derived Protein Microarrays for Diagnostics and Screening, N. Rupcich and J.D. Brennan
Printing and QC of Functional Protein Microarrays, D. Shen, F.X. Zhou, and B. Schweitzer
Protein Engineering for Surface Attachment, A. Girish, G.Y.J. Chen, and S.Q. Yao
Protein In Situ Arrays through Cell-Free Protein Synthesis, M. He, F. Khan, E. Palmer, M. Wang, and M.J. Taussig
Detection Methods for Protein Microarrays
Fluorescent Detection Methods for Protein Microarrays, S. Roman and S. Clarke
Functional Analysis of Protein Interactions Using Surface Plasmon Resonance-Based Microarrays, A. McWhirter and S. Löfås
Leaving the Surface Behind: At the Intersection of Protein Microarrays and Mass Spectrometry, D.P. Chandler, D.S. Schabacker, S. Bavykin, and I.M. Gavin
High-Resolution Label-Free Detection Applied to Protein Microarray Research, L.G. Laing and B. Cunningham
Applications of Functional Protein Microarrays
Studying Protein–Protein Interactions with Protein Microarrays: Rapid Identification of 14-3-3 Protein Binding Partners, J.-i. Satoh
A Combined Force of Chemical Genetics and Protein Microarrays, H. Zhu and J. Huang
Antibody Profiling for Protein Drug Development and Clinical Development, S.H. Herrmann
Humoral Response Profiling Using Protein Microarrays, A. Sreekumar, B.S. Taylor, X. Wang, D. Lubman, and A.M. Chinnaiyan
DNA Interactions with Arrayed Proteins, M. Snapyan and V. Sakanyan
G Protein–Coupled Receptor Microarrays for Drug Discovery, J. Salon, M. Johnson, B. Rasnow, G. Biddlecome, Y. Hong, B. Webb, Y. Fang, and J. Lahiri
Kinase Substrate Identification Using Yeast Protein Microarrays, G. Devgan and M. Snyder
Bioinformatics & Data Analysis
Protein Microarray Image Analysis, M. Ruan
The Analysis of Protein Arrays, B. Love
Evaluating Precision and Recall in Functional Protein Arrays, K. Robison
Visualization of Protein Microarray Data, K. Clancy Index

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"The first integrated reference for functional protein microarrays, this book helps you not only meet the challenges but also excel in your field."

– In Anticancer Research, September/ October 2007, Vol. 27, No. 5A