1st Edition

Fundamentals of Fluorescence Imaging

Edited By Guy Cox Copyright 2019
    476 Pages 100 Color & 52 B/W Illustrations
    by Jenny Stanford Publishing

    476 Pages 100 Color & 52 B/W Illustrations
    by Jenny Stanford Publishing

    Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism.

    This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.

    What Is Fluorescence?

    Scott Kable

    The Ploem Fluorescence Microscope

    Guy Cox

    The Confocal Fluorescence Microscope

    Stephen Cody and Guy Cox

    Multiphoton Fluorescence Microscopy

    Mark Cannell, Guy Cox, and Warren Zipfel


    Tony Henwood

    Fluorescent Proteins

    Anya Salih

    Dyes for Labeling Organelles and Compartments

    Iain Johnson

    Indicator and Reporter Dyes

    Iain Johnson

    New Approaches to Cancer Therapy Using In Vivo Fluorescent- Protein Imaging

    Robert Hoffman

    Fluorescence Lifetime Imaging

    Chittanon Buranachai, John P. Eichorst, Kei Wei Teng, and Robert M. Clegg

    Dynamic Experiments: FRAP and Photoconversion

    Kelly Rogers and Sarah Ellis

    Förster Resonant Energy Transfer (FRET)

    Vinod Jyothikumar, Yuansheng Sun, and Ammasi Periasamy

    Recording the Fluorescent Image

    James Pawley

    Practical Aspects of Localisation Microscopy

    Mark Cannell, Christian Soeller, and David Baddeley

    Super-Resolution Optical Microscopy with Structured Illumination

    Trevor Smith and Liisa Hirvonen

    STED Nanoscopy

    Christian Wurm, Andreas Schoenle, and Christian Eggeling


    Guy Cox is honorary associate professor at the School of Medical Science and Australian Centre for Microscopy & Microanalysis, University of Sydney, Australia. He has been and is a member of several learned and professional societies, including the Australasian Society for Phycology and Aquatic Botany, Australian Microscopy and Microanalysis Society, Australian Speleological Federation, Phycological Society of America, USA, and Royal Microscopical Society, UK. Prof. Cox is also on the editorial board of Micron.

    "Fundamentals of Fluorescence Imaging is one of those must-need textbooks for students in biomedical sciences, and I will definitely use it as such. In easy-to-read language understandable to the non-physicists or non-microscopists but in a complete way, it gives an overview of the most used fluorescence microscopy techniques. What is great about this book is also that it combines the technological part with very practical topics: sample preparation (chapter 5) and labeling of specific organelles (chapter 6–8). The book is fun to read and very instructive. I will implement it in my teaching program for sure."

    Prof. Dr. Marc A. M. J. van Zandvoort
    Maastricht University, the Netherlands

    "This is an excellent book on the fundamentals of fluorescent imaging. There are very few books that provide the depth of coverage this one does. The book is clearly written without mathematics."

    Omer Iqbal, MD, FACC, FESC
    Loyola University Medical Center, USA