Fundamentals of Fluorescence Imaging  book cover
1st Edition

Fundamentals of Fluorescence Imaging

Edited By

Guy Cox

ISBN 9789814774857
Published April 9, 2019 by Jenny Stanford Publishing
476 Pages 100 Color & 52 B/W Illustrations

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USD $179.95

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Book Description

Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism.

This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.

Table of Contents

What Is Fluorescence?

Scott Kable

The Ploem Fluorescence Microscope

Guy Cox

The Confocal Fluorescence Microscope

Stephen Cody and Guy Cox

Multiphoton Fluorescence Microscopy

Mark Cannell, Guy Cox, and Warren Zipfel


Tony Henwood

Fluorescent Proteins

Anya Salih

Dyes for Labeling Organelles and Compartments

Iain Johnson

Indicator and Reporter Dyes

Iain Johnson

New Approaches to Cancer Therapy Using In Vivo Fluorescent- Protein Imaging

Robert Hoffman

Fluorescence Lifetime Imaging

Chittanon Buranachai, John P. Eichorst, Kei Wei Teng, and Robert M. Clegg

Dynamic Experiments: FRAP and Photoconversion

Kelly Rogers and Sarah Ellis

Förster Resonant Energy Transfer (FRET)

Vinod Jyothikumar, Yuansheng Sun, and Ammasi Periasamy

Recording the Fluorescent Image

James Pawley

Practical Aspects of Localisation Microscopy

Mark Cannell, Christian Soeller, and David Baddeley

Super-Resolution Optical Microscopy with Structured Illumination

Trevor Smith and Liisa Hirvonen

STED Nanoscopy

Christian Wurm, Andreas Schoenle, and Christian Eggeling

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Guy Cox is honorary associate professor at the School of Medical Science and Australian Centre for Microscopy & Microanalysis, University of Sydney, Australia. He has been and is a member of several learned and professional societies, including the Australasian Society for Phycology and Aquatic Botany, Australian Microscopy and Microanalysis Society, Australian Speleological Federation, Phycological Society of America, USA, and Royal Microscopical Society, UK. Prof. Cox is also on the editorial board of Micron.


"Fundamentals of Fluorescence Imaging is one of those must-need textbooks for students in biomedical sciences, and I will definitely use it as such. In easy-to-read language understandable to the non-physicists or non-microscopists but in a complete way, it gives an overview of the most used fluorescence microscopy techniques. What is great about this book is also that it combines the technological part with very practical topics: sample preparation (chapter 5) and labeling of specific organelles (chapter 6–8). The book is fun to read and very instructive. I will implement it in my teaching program for sure."

Prof. Dr. Marc A. M. J. van Zandvoort
Maastricht University, the Netherlands

"This is an excellent book on the fundamentals of fluorescent imaging. There are very few books that provide the depth of coverage this one does. The book is clearly written without mathematics."

Omer Iqbal, MD, FACC, FESC
Loyola University Medical Center, USA