Fundamentals of Fluorescence Imaging: 1st Edition (Hardback) book cover

Fundamentals of Fluorescence Imaging

1st Edition

Edited by Guy Cox

Pan Stanford

300 pages

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Hardback: 9789814774857
pub: 2019-04-30
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Fluorescence imaging, at macro, micro and sub-micro scales, has revolutionized biological science in the past 30 years. Immuno-labeling has provided the precise targeting of molecules in the fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable the imaging of dynamic changes in cell metabolism.

This book covers for the first time imaging at all scales, from macro to sub-micro (super-resolution). The authors include Robert Clegg, legendary teacher and researcher (who sadly passed away during the editorial process); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling, experts in STED nanoscopy; and many other famous participants in this field. All the contributors are at the cutting edge of their field.

Table of Contents

What Is Fluorescence?

Scott Kable

The Ploem Fluorescence Microscope

Guy Cox

The Confocal Fluorescence Microscope

Stephen Cody and Guy Cox

Multiphoton Fluorescence Microscopy

Mark Cannell, Guy Cox, and Warren Zipfel


Tony Henwood

Fluorescent Proteins

Anya Salih

Dyes for Labeling Organelles and Compartments

Iain Johnson

Indicator and Reporter Dyes

Iain Johnson

New Approaches to Cancer Therapy Using In Vivo Fluorescent- Protein Imaging

Robert Hoffman

Fluorescence Lifetime Imaging

Chittanon Buranachai, John P. Eichorst, Kei Wei Teng, and Robert M. Clegg

Dynamic Experiments: FRAP and Photoconversion

Kelly Rogers and Sarah Ellis

Förster Resonant Energy Transfer (FRET)

Vinod Jyothikumar, Yuansheng Sun, and Ammasi Periasamy

Recording the Fluorescent Image

James Pawley

Practical Aspects of Localisation Microscopy

Mark Cannell, Christian Soeller, and David Baddeley

Super-Resolution Optical Microscopy with Structured Illumination

Trevor Smith and Liisa Hirvonen

STED Nanoscopy

Christian Wurm, Andreas Schoenle, and Christian Eggeling

About the Editor

Guy Cox is honorary associate professor at the School of Medical Science and Australian Centre for Microscopy & Microanalysis, University of Sydney, Australia. He has been and is a member of several learned and professional societies, including the Australasian Society for Phycology and Aquatic Botany, Australian Microscopy and Microanalysis Society, Australian Speleological Federation, Phycological Society of America, USA, and Royal Microscopical Society, UK. Prof. Cox is also on the editorial board of Micron.

Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Life Sciences / Biology / Molecular Biology
SCIENCE / Physics