This is a concise guide to the combined use of classical and molecular methods for the genetic analysis and breeding of fungi. It presents basic concepts and experimental designs, and demonstrates the power of fungal genetics for applied research in biotechnology and phytopathology. Case studies of Saccharomyces cerevisiae, Candida albicans, Aspergillus niger, Neurospora crassa, Podospora anserina, Phytophthora infestans and others are included.
Part 1 Genetic principles: biology of fungi; mutation; meiotic recombination; somatic recombination; molecular genetic analysis; the meiotic system of fungi; extrachromosomal and transposable genetic elements; genetic transformation and vector developments in filamentous fungi; mapping and breeding strategies. Part 2 Case studies of genetic analyses: mitochondrial genetics of saccharomyces cerevisiae - the way from genetic crosses to transposable elements; construction of a physical map of the candida albicans genome; use of heterologous gene as marker for genetic analysis in aspergillus niger; mutation in neurospora crassa - from x-rays to RIP; genetic analysis and mapping of avirulence genes in magnaporte grisea; genetic analysis of senescence in podospora anerina; horizontal transmission in fungal populations; genetic analysis in the imperfect fungus cladosporium fulvum - the development of a map-based strategy for cloning of genes involved in pathogenicity; production and analysis of meiotic mutants in corpinus cinereus; a case study in fungal development and genetics - schizophyllum commune; genetics of phycomyces; genetic analysis of the oomycetous fungus, phytophthora infestans.