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Fungal Protoplasts
Applications in Biochemistry and Genetics



ISBN 9780824771126
Published February 20, 1985 by CRC Press
368 Pages

 
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Book Description

Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts.

Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.

Table of Contents

Preface
Contributors

THE PROTOPLAST SYSTEM
The Fungal Cell Wall
Vladimir Farkaš

Mycolytic Enzymes
John F. Peberdy

Factors Influencing Protoplast Isolation
Barry Davis

Protoplasts from Fungal Spores
Cees J. Bos

Protoplasts from Entomophthorales
Richard A. Nolan

PROTOPLASTS AS TOOLS IN PHYSIOLOGICAL AND BIOCHEMICAL STUDIES
Cell Wall Regeneration and Protoplast Reversion
Oldrich Necas and Augustin Svoboda

The Protoplast Membrane and Antifungal Drugs
David Kerridge

Metabolic Properties of Protoplasts
Susan Isaac

Protoplasts and Secondary Metabolism
Joan W. Bennett

PROTOPLASTS AS GENETIC TOOLS
Protoplast Fusion and Genetic Analysis in Cephalosporium
John A. Birkett and Paul F. Hamlyn

Protoplast Fusion and Incompatibility in Aspergillus
James H. Croft

Interspecies Hybridization after Protoplast Fusion in Aspergillus
Ferenc Kevei and John F. Peberdy

Protoplast Fusion and Interspecies Hybrization in Penicillium
Jozef Anné and John F. Peberdy

Protoplast Fusion in Yeasts
Lajos Ferenczy

Transfer of Cytoplasmic Genetic Elements by Protoplast Fusion
Lajos Ferenczy

Some Aspects of Yeast Transformation
Albert Hinnen

Perspectives for Protoplasts in Genetics of Industrially Important Fungi
Christopher Ball

Index

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