Fungi: Experimental Methods In Biology, Second Edition, 2nd Edition (Paperback) book cover


Experimental Methods In Biology, Second Edition, 2nd Edition

By Ramesh Maheshwari

CRC Press

358 pages | 5 Color Illus. | 200 B/W Illus.

Purchasing Options:$ = USD
Paperback: 9781138199255
pub: 2016-11-16
SAVE ~$12.99
Hardback: 9781439839034
pub: 2011-09-21
SAVE ~$33.00
eBook (VitalSource) : 9780429106521
pub: 2016-04-19
from $28.98

FREE Standard Shipping!


Fungi are now at the forefront of research on mechanisms in gene silencing, biological rhythm, mating processes, biogenesis of intracellular organelles, adaptations to hostile habitats, structure of natural populations, and speciation. Because of their small genomes, fungi are being used in "systems biology" to understand the connections between genes, proteins, and metabolic and signaling pathways. The ease with which yeasts and fungi can be cultivated in simple nutritive media has also made these eukaryotic organisms the choice material for basic and applied research.

Fungi: Experimental Methods in Biology, Second Edition presents the latest information on fungal biology generated through advances in genetics, molecular biology, and biochemistry. It gives an account of real experiments that have been carried out on the diverse lifestyles of these organisms. Following in the footsteps of its highly-praised predecessor, this book continues to be a comprehensive review of the state of our knowledge about how fungi function.

Examining both unicellular and multicellular fungi, this accessible book covers:

  • Special features of fungi
  • Interaction of fungi with other organisms
  • Use of fungi as sources of chemicals for human health and welfare
  • Model fungi in research
  • Gene manipulation
  • Adaptations
  • Natural populations

Throughout, the book draws attention to unsolved questions and to the opportunities offered by the diversity of fungi. Written by a prominent mycologist, it provides an excellent introduction and reference for beginning researchers as well as for experienced professionals.

What’s New in This Edition:

  • New chapters on spores’ dormancy, germination, and uses; fungi as scavengers; and fungi as chemical factories
  • All chapters substantially revised, updated, and rewritten, in particular The Hyphal Mode of Life, Fungi as Symbiotic Partners, and Fungi as Plant Pathogens
  • New material on the use of yeast for functional analysis of genomes; the use of Neurospora in cytogenetics and genes controlling conidiation; and the identification of the clock gene
  • A new glossary to reinforce important concepts 


"… Following in the footsteps of its highly-praised predecessor, this book continues to be a comprehensive review of the state of our knowledge about how fungi function."

—Northeastern Naturalist, December 2012

Praise for the First Edition:

"…an important volume. …a comprehensive review of the state of our knowledge about how fungi function as individuals, populations, and members of the global economy of nature. Maheshwari has done an exemplary job. …the integration of information from such a broad area and the general clarity of presentation found in this volume deserve wide readership among mycologists, plant pathologists, geneticists, and molecular biologists."

—David S. Yohalem, East Malling Research, Kent, UK

"The book is simply written and easy to digest, which is aided by the consistent editorial style throughout the book."

—N. Louise Glass, Mycopathologia, 2006

"The most important feature of the book is its comprehensive nature…. This book, in describing the modernization of mycology that has come from advances in biochemistry, genetics, and molecular biology, will serve as an excellent introduction and reference for students and investigators having a background in these subjects and a particular interest in the fungi…. As such, the book sets an agenda for fungal research in the 21st century."

—Rowland H. Davis, University of California, Irvine

"It should prove useful both for undergraduate students and postgraduate researchers."

—David J. Adams, Microbiology Today

Table of Contents

The Unique Features of Fungi

The Hyphal Mode of Life

The Multinuclear Condition

Spores: Their Dormancy, Germination, and Uses

Fungi in Biosphere and Human Health

Fungi as Scavengers

Fungi as Symbiotic Partners

Fungi as Plant Pathogens

Fungi as Chemical Factories

Gene Silencing

Transformation and Discovery of Gene-Silencing Phenomena

Model Organisms

Yeast: A Unicellular Paradigm for Complex Biological Processes

Neurospora: A Gateway to Biology

Aspergillus nidulans: A Model for Study of Form and Asexual Reproduction

Ustilago maydis and Other Fungi as Models of Sexual Reproduction


Photoresponses and Circadian Rhythm

Thermophilic Fungi: Eukaryotic Life at High Temperature


Species—Their Diversity and Populations


A Glossary of Mycological and Interdisciplinary Terms

Appendix: Naming, Defining, and Broadly Classifying Fungi


About the Author/Editor

Ramesh Maheshwari received his PhD degree from the University of Wisconsin–Madison. He did his postdoctoral work at the University of Michigan–Ann Arbor and at Stanford University, California. He has held academic appointment as a professor of biochemistry at the Indian Institute of Science in Bangalore. He is the author of Fungi: Experimental Methods in Biology and over 100 scientific papers on fungal and plant physiology. The thrust of his research over a span of 35 years was on the physiology and enzymes of thermophilic fungi and the genetics and ecology of Neurospora.

About the Series


Learn more…

Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Life Sciences / Botany
SCIENCE / Life Sciences / Biology / Microbiology
SCIENCE / Life Sciences / Biology / Molecular Biology
SCIENCE / Life Sciences / General