The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions of gene regulation and function made through bioinformatics. Applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture, are also covered in the book.
Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource to both undergraduates and lecturers of biological and biomedical science courses.
'Had I ever been a practical molecular biologist, this is the book that would reassure me of the principles behind my experiments. Furthermore, if, for example, I wanted to find the transcription starting point of a gene, this would tell me the theory behind how to do it. Thus it is not a lab cookbook or manual, but a very user-friendly and helpful adviser. I was rather impressed with this book and will suggest it as a text, not only for my second and third year students but also for our MSc molecular genetics course.' - The Times Higher Education Supplement, February 23rd 2007
'Instructors seeking a text for a course in molecular biology would be
wise to consider this one. The authors are to be commended for a readable and
well organized presentation.
This is a strong effort. The presentation is logical, the level correct. Were I
to teach a course in this area, this book would be high on my list.' - Eugene A Davidson, PhD, Georgetown University School of Medicine, Doody's Reviews, April 2007
'The writing is clear and concise and, importantly for beginners, the essential information is not lost amongst a myriad of details. The instructor will find an excellent combination of traditional and up-to-date topics treated and will be able to easily complement this with information from the literature or laboratory.' - BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION, Vol. 36, 2008
2. Genome Organisation
3. Key Tools for Gene Cloning
4. Gene Identification and DNA Libraries
5. Screening DNA Libraries
6. Further Routes to Gene Identification
7. Sequencing DNA
9. Production of Proteins from Cloned Genes
10. Gene Cloning in the Functional Analysis of Proteins
11. The Analysis of the Regulation of Gene Expression
12. The Production and Uses of Transgenic Organisms
13. Forensic and Medical Applications