Presenting all preclinical and clinical information available on genetically engineered toxins, this unique, single-source reference provides the most up-to-date methods and practical examples for conducting clinical studies in toxin molecular biology.;Reviewing difficult problems and their solutions, Genetically Engineered Toxins discusses techniques for clo;ning, expressing, and purifying recombinant toxins and genetically modified recombinant toxins; documents structure-function relationships in toxins, including comparative information; supplies theory and illustrations of chimeric toxins; delineates the preclinical assessments of new reagents; and summarizes approaches to drug design.;With over 1100 literature citations, Genetically Engineered Toxins is an invaluable resource for biochemists, molecular biologists, biotechnologists, pharmacologists, toxicologists, X-ray crystallographers, enzymologists, oncologists, hematologists, immunologists, rheumatologists, botanists, and graduate-level students in molecular biology, biotechnology, and clinical oncology courses.
Table of Contents
Strategies and techniques - general methods, Robert F. Weaver, cloning strategies, Richard Intres and John W. Crabb, protein engineering strategies, Marc Whitlow, general strategies in in vivo animal modelling, Daniel A. Vallera and Bruce R. Blazar; ricin - molecular cloning of ricin, Lynne R. Roberts, et al, expression of plant-derived ribosome-inactivating proteins in heterologous systems, Michael Piatak, Jr, and Noriyuki Habuka, the structure of plant toxins as a guide to rational design, Jon D. Robertus, chemical and genetic characterization of the enzymatic activity associated with ricin A chain, Lawrence Greenfield, chimeric proteins containing ricin A chain, J. Michael Lord, et al; plant hemitoxins - studies on ribosome-inactivating proteins from Saponaria officinalis, Marco R. Soria, et al, cloning and expression of trichosanthin and - momorcharin cDNA, Pang-Chui shaw, et al, cloning and expression of a Luffa ribosome-inactivating-related protein, Bi-Yu Li and S. Ramakrishnan; fungal ribotoxins - the Aspergillus ribonucleolytic toxins (ribotoxins), Bernard Lamy, et al, an efficient expression system for -sarcin in Escherichia coli, Yaeta Endo, et al; diphtheria toxin - diphtheria toxin cloning and expression in corynebacterium diphtheriae, Lawrence Greenfield, diphtheria toxin expression in Escherichia coli, Lawrence Greenfield, the structure of diphtheria toxin as a guide to rational design, Peter J. Nicholls and Richard J. Youle, protein engineering of diphtheria toxin - development of receptor-specific cytotoxic agents for the treatment of human disease, John R. Murphy , et all, in vivo studies with chimeric toxins - interleukin-2 fusion toxins as immunosuppressive agents, Michael E. Shapiro, et al, initial clinical experiences with an interleukin-2 fusion toxin (DAB 486-IL-2), Carole M. Meneghetti and C.F. Le Maistre; pseudomonas exotoxins - pseudomonas aeruginosa exotoxin A, G. Jiliani Chaudry, et al, expression of growth factor - toxin fusion proteins, Gwynneth M. Edwards, et al, the structure of pseudomonas exotoxin A as a guide to rational design, Peter J. Nichols and Richard J. Youle, generation of chimeric toxins, David FitzGerald, et al; conclusions - genetically engineered toxins in perspective, Sjur Olsnes and Arthur E. Frankel, Appendix - primary amino acid sequences of toxins, Paul Sehnke and Alexander Tonevistsky.
Arthur E. Frankel