Genome Visualization by Classic Methods in Light Microscopy: 1st Edition (Hardback) book cover

Genome Visualization by Classic Methods in Light Microscopy

1st Edition

By Jean-Marie Exbrayat

CRC Press

216 pages | 34 B/W Illus.

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pub: 2000-11-27
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Description

Visualization of nucleic acids has become indispensable to studying cells, tissues, and organisms. Certain techniques even permit quantification of DNA and/or RNA distribution in tissues, but few current analytical books cover the numerous methods for DNA and RNA visualization. This book provides insight into several classic techniques, histological as well as histochemical, that can be used to appreciate the nucleic acid status of the cell as well as to provide an overview of RNA and DNA distribution in cells and tissues.

Genome Visualization by Classic Methods in Light Microscopy begins with an introduction to DNA and RNA, followed by general visualization principles. The subsequent chapters describe: how to prepare tissues for staining; the principles, chemical formulas, and procedures for nuclear dye, fluorescent dye, and histochemical methods; directions to observe the products of the stained reactions; and more. Each protocol is presented as easy-to-follow directions and the author includes cautionary notes and points to consider. The final section provides color photographs of various tissues in which the staining method, fixative, and observations are noted.

A theoretical and practical book, Genome Visualization by Classic Methods in Light Microscopy allows you to understand which technique is most useful for your particular problem. Laboratory protocols are provided for you to follow, chemical structures and principles are provided for you to understand the technique, and the book is organized so you can find the necessary information when needed. This is the essential guide to understanding and executing visualization techniques for nucleic acids.

Table of Contents

GENERAL INTRODUCTION

ABBREVIATIONS

PRINCIPLES

Nucleic Acids

General Principles

TISSUE PREPARATION

Sampling

Fixation

Embedding

Sections

Adhesion of Sections

Deparaffining and Hydration

Collodioning

Smears

Cell Cultures

STAINING

Nuclear Dyes

Methods Used to Increase Contrast

HISTOCHEMICAL METHODS

General Methods

Basophilic Reactions

Feulgen and Rossenbeck Nuclear Reaction

Other Reactions

FLUORESCENT METHODS

Fluorescent Methods

Staining Methods

OBSERVATION PHASES

Mounting

Photonic Microscope

PREPARATION OF PRODUCTS

Fixatives

Dyes

Alcohol Dilutions

PROTOCOLS

Introduction

Fixation of Organs for Nucleic Acid Visualization

Staining

EXAMPLES OF STAINING METHODS

GLOSSARY

INDEX

About the Originator

About the Series

Methods in Visualization

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
SCI017000
SCIENCE / Life Sciences / Cytology
SCI029000
SCIENCE / Life Sciences / Genetics & Genomics
SCI047000
SCIENCE / Microscopes & Microscopy