Handbook of Carbohydrate Engineering: 1st Edition (Hardback) book cover

Handbook of Carbohydrate Engineering

1st Edition

Edited by Kevin J. Yarema

CRC Press

944 pages | 265 B/W Illus.

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The Handbook of Carbohydrate Engineering provides an overview of the basic science, theory, methods, and applications of this broad, interdisciplinary field. The text provides background information along with practical knowledge for current and future research methodologies used in the characterization and synthesis of various carbohydrates. This multidisciplinary perspective involves aspects of basic biology, synthetic chemistry, enzymology, complex instrumentation, and sophisticated modeling.

The book presents the fundamentals of carbohydrate engineering, addressing concepts in structure, biosynthesis, and biological functions for a variety of carbohydrates with a particular emphasis on mammalian glycoproteins and their N-linked oligosaccharides, glycolipids, sialic acid, as well as polysaccharides from both eukaryotes and bacteria. It describes glycosylation processes found in nature and surveys methods to manipulate these metabolic systems in living cells both for the improved production of carbohydrates and to give these molecules novel properties. Subsequent sections discuss the various methods of purification, synthesis, modification, and analysis used to create and manipulate carbohydrates in the laboratory; these approaches include chemical-enzymatic synthesis, small-molecule cell-based strategies, as well as complete chemical synthesis.

The Handbook of Carbohydrate Engineering also focuses on practical applications for carbohydrates. It emphasizes methods to characterize glycosylation pathways and expounds upon the role of carbohydrates in health and disease, a significant - and rapidly growing - area of research. World-renowned experts discuss biomedical applications, including the development of vaccines, therapeutics, glycomimetics, antibody engineering, drug delivery, tissue engineering and organ regeneration, and diagnostic agents. Several chapters also cover important applications in agriculture, industry, food technology, and environmental remediation.

Table of Contents

Mammalian Glycosylation: An Overview of Carbohydrate Biosynthesis; H. Chen, Z. Wang, Z. Sun, E.J. Kim, and K.J. Yarema

Patterning of Lectins of Vero and MDCK Cells and Influenza Viruses: The Search for Additional Virus/Cell Interactions; E. M. Rapoport, L.V. Mochalova, H.-J. Gabius, J. Romanova, and N.V. Bovin

O-Acetyl Sialic Acids in Parasitic Diseases; A.K. Chava, M. Chatterjee, and C. Mandal

Congenital Disorders of Glycosylation; J. Ko_cielak

Role of Milk Carbohydrates in Preventing Bacterial Adhesion; P. Hueso, S. Martín-Sosa, and M.-J. Martín

Structural Characterization of Glycosaminoglycans; K. Pojasek, R. Raman, and R. Sasisekharan

Carrageenans: Structural and Conformational Studies; C.A. Stortz

Computational Modeling in Glycosylation; M.P. Murrell, K.J. Yarema, and A. Levchenko

Biosynthetic Pathways for Differential Expression of Functional Chondroitin Sulfate and Heparan Sulfate; S. Mizumoto, T. Uyama, T. Mikami, H. Kitagawa, and K. Sugahara

Large-Scale Production of Oligosaccharides Using Engineered Bacteria; S. Koizumi

Fructan Biosynthesis in Genetically-Modified Plants; A.J. Cairns and S.J. Perret

Chinese Hamster Ovary (CHO) Glycosylation Mutants for Glycan Engineering; P. Stanley and S.K. Patnaik

Biochemical Engineering of Sialic Acids; S. Hinderlich, C. Oetke, and M. Pawlita

Glycosylation in Native and Engineered Insect Cells; K. Viswanathan and M.J. Betenbaugh

N-Glycan Engineering in Yeasts and Fungi: Progress Toward Human-Like Glycosylation; N. Callewaert, W. Vervecken, S. Geysens and R. Contreras

Bacterial Capsules: A Route for Polysaccharide Engineering; C.M. Taylor and I.S. Roberts

Chemo-Biological Approach to Modification of the Bacterial Cell Wall; R. Sadamoto and S.-I. Nishimura

Engineering Bacterial Biopolymers for the Biosorption of Heavy Metals; H. Bach and D.L. Gutnick

Treatment of Wastewaters with the Biopolymer Chitosan; H.K. No, W. Prinyawiwatkul, and S.P. Meyers

The Role of Glycosylation in Engineered Antibodies; S.L. Morrison

Recent Developments in the Synthesis of Oligosaccharides by Hyperthermophilic Glycosidases; M. Moracci, B. Cobucci-Ponzano, G. Perugino, A. Giordano, A. Trincone, and M. Rossi

Modification of Plant Cell Wall Polysaccharides Using Enzymes from Aspergillus; R.P. de Vries, M.C. McCann, and J. Visser

ß-Glucosidases from Filamentous Fungi: Properties, Structure, and Applications; J. Eyzaguirre, M. Hidalgo, and A. Leschot

Sialic Acids and Sialylmimetics: Useful Chemical Probes of Sialic Acid-Recognizing Proteins; J.C. Wilson, M.J. Kiefel, and M. von Itzstein

Engineering Carbohydrate Scaffolds into the Side Chains of Amino Acids and Use in Combinatorial Synthesis; F. Schweizer

Effects of Short- and Long-Chain Fructans on Large Intestinal Physiology and Development of Pre-Neoplastic Lesions in Rats; M. Poulsen

Alginate as Drug a Delivery Carrier; R. Pandey and G.K. Khuller

Chitosan-Based Nonviral Vectors for Gene Delivery; W.G. Liu, W.W. Lu, and K.D. Yao

Polysaccharides in Tissue Engineering Applications; D.W. Hutmacher, David T.W. Leong, and F. Chen


About the Authors/Editor

Kevin J. Yarema

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biochemistry
MEDICAL / Biotechnology
MEDICAL / Pharmacy