Handbook of HLA Typing Techniques is an authoritative collection of HLA phenotyping and genotyping techniques to be used at the bench level and as a reference. The information presented, much of it previously unpublished, was written by leading authorities in the field of transplantation. Each chapter provides detailed methodologies, notes on the interpretation of tests, reference material, and appendices.
Phenotyping topics covered include microlymphocytotoxicity assay, monoclonal antibodies, complement testing, serum screening for HLA antibodies, lymphocytotoxic crossmatching, and mixed and primed lymphocyte testing. Genotyping topics discussed include well-established RFLP analysis and polymerase chain reaction applications of PCR-SSO, PCR-RFLP, PCR-SSP, and PCR fingerprinting and related DNA heteroduplex technologies. Handbook of HLA Typing Techniques is an indispensable sourcebook for all researchers, practitioners, and students in the international tissue typing and human immunogenetics community.
Introduction. PCR-RFLP (H. Inoko and M. Ota). HLA-DR and -DQ Typing by DNA-RFLP Analysis (J.L. Bidwell, E.A. Bidwell, N.A.P. Wood, M.C. Pursall, B.A. Bradley, I. Crosby, and J.D. Bignon). Rapid HLA-DR-Dw and DP Matching by PCR Fingerprinting and Related DNA Heteroduplex Technologies (J.L. Bidwell, T.M. Clay, N.A.P. Wood, M.C. Pursall, A.F. Martin, B.A. Bradley, and K.M. Hui). A Comprehensive HLA-DRB, -DQB, and -DPB Oligotyping Procedure by Hybridization with Sequence-Specific Oligonucleotide Probes (J.-M. Tiercy, C. Grundschober, M. Jeannet, and B. Mach). HLA-DR Typing by Polymerase Chain Reaction Amplification with Sequence-Specific Primers (PCR-SSP) (O. Olerup and H. Zetterquist). Serological Typing of HLA-A, -B, and -C Antigens (D.J. McCloskey, J. Brown, and C. Navarrete). HLA-DR and -DQ Serotyping (J. Brown, D. McCloskey, and C. Navarrete). Definition of HLA-Dw Determinants Using Homozygous Typing Cells and the Mixed Lymphocyte Culture (C. Navarrete, J. Brown, D. McCloskey, and D. Jaraquemada). Definition of HLA-Dw and HLA-DPw Determinants by the Primed Lymphocyte Test (C. Navarrete, D.J. McCloskey, and J. Brown). Appendix: HLA Class II Nucleotide Sequences, 1992 (S.G.E. Marsh and J.G. Bodmer). Index.