The Handbook of Neurophotonics provides a dedicated overview of neurophotonics, covering the use of advanced optical technologies to record, stimulate, and control the activity of the brain, yielding new insight and advantages over conventional tools due to the adaptability and non-invasive nature of light.
Including 30 colour figures, this book addresses functional studies of neurovascular signaling, metabolism, electrical excitation, and hemodynamics, as well as clinical applications for imaging and manipulating brain structure and function. The unifying theme throughout is not only to highlight the technology, but to show how these novel methods are becoming critical to breakthroughs that will lead to advances in our ability to manage and treat human diseases of the brain.
- Provides the first dedicated book on state-of-the-art optical techniques for sensing and imaging across at the cellular, molecular, network, and whole brain levels.
- Highlights how the methods are used for measurement, control, and tracking of molecular events in live neuronal cells, both in basic research and clinical practice.
- Covers the entire spectrum of approaches, from optogenetics to functional methods, photostimulation, optical dissection, multiscale imaging, microscopy, and structural imaging.
- Includes chapters that show use of voltage-sensitive dye imaging, hemodynamic imaging, multiphoton imaging, temporal multiplexing, multiplane microscopy, optoacoustic imaging, near-infrared spectroscopy, and miniature neuroimaging devices to track cortical brain activity.
Table of Contents
Part I Function and Structural Neurophotonic Imaging
Chapter 1 ◾ Miniaturized Optical Neuroimaging Systems 3
Hang Yu, Janaka Senarathna, Betty M. Tyler, Nitis h V. Thakor, and Arvi nd P. Pathak
Chapter 2 ◾ Functional Imaging with Light-Sheet Microscopy 21
Raghav K. Chhetri and Philipp J. Keller
Chapter 3 ◾ Two-Photon Microscopy in the Mammalian Brain 55
Hod Dana and Shy Shoham
Chapter 4 ◾ Light Field Microscopy for In Vivo Ca2+ Imaging 81
Tobi as Nöbauer and Alip asha Vaziri
Chapter 5 ◾ Genetically Encoded Activity Indicators 113
Chenchen Song and Thomas Knöpfel
Chapter 6 ◾ Functional Optoacoustic Imaging 129
Chapter 7 ◾ Imaging Deep in the Brain with Wavefront Engineering 147
Roarke Horstmeyer, Maximi llian Hoffmann, Haowen Ruan, Benjami n Judkewitz,
and Changhuei Yang
Chapter 8 ◾ Nanoscopic Imaging to Understand Synaptic Function 173
Daniel Choquet and Anne-Sophie Hafner
Chapter 9 ◾ Chemical Clearing of Brains 191
Klaus Becker, Chris tian Hahn, Nina Jährling, Marko Pende,
Inna Sabdyusheva-Litsc hauer, Saiedeh Saghafi, Martina Wanis , and Hans-Ulric h Dodt
Chapter 10 ◾ Advanced Light-Sheet Microscopy to Explore Brain
Structure on an Organ-Wide Scale 221
Ludovic o Silvestri and Francesc o S. Pavone
Part II Neurophotonic Control and Perturbation
Chapter 11 ◾ Optogenetic Modulation of Neural Circuits 235
Mathias Mahn, Oded Klavi r, and Ofer Yizhar
Chapter 12 ◾ Molecular Photoswitches for Synthetic Optogenetics 271
Shai Berlin and Ehud Y. Isacoff
Chapter 13 ◾ Applications of Nanoparticles for Optical Modulation of
Neuronal Behavior 293
Chiara Pavi olo, Shaun Gietman, Daniela Duc, Sim on E. Moulton, and
Paul R. Stoddart
Chapter 14 ◾ Optical Stimulation of Neural Circuits in Freely Moving
Leore R. Heim and Eran Stark
Chapter 15 ◾ Holographic Optical Neural Interfaces (HONIs) 333
Shani Rosen, Shir Paluch, and Shy Shoham
Chapter 16 ◾ Multi-Photon Nanosurgery 353
Anna Letizia Allegra Masc aro and Francesc o Saverio Pavone
Part III Clinical and Human Neurophotonics
Chapter 17 ◾ High Resolution Diffuse Optical Tomography of the
Human Brain 369
Muriah D. Wheelock and Adam T. Eggebrecht
Chapter 18 ◾ Human Brain Imaging by Optical Coherence Tomography 399
Caroline Magnain, Jean C. Augustinack, Davi d Boas, Bruce Fisc hl, Taner Akkin,
Ender Konukoglu, and Hui Wang
Chapter 19 ◾ Acousto-Optic Cerebral Monitoring 439
Mic hal Balberg and Revi tal Pery-Shechter
Chapter 20 ◾ Neurophotonic Vision Restoration 459
Adi Schejter Bar-Noam and Shy Shoham
Chapter 21 ◾ Optical Cochlear Implants 473
C.-P. Ric hter, Y. Xu, X. Tan, N. Xia, and N. Suematsu
Chapter 22 ◾ Label-Free Fluorescence Interrogation of Brain Tumors 503
Brad A. Hartl, Shami ra Sridharan, and Laura Marcu
Chapter 23 ◾ Higher Harmonic Generation Imaging for Neuropathology 527
Nikolay Kuzmi n, Sander Idema, Eleonora Aronic a, Philip C. de Witt Hamer,
Pieter Wess eling, and Marie Louis e Groot
Francesco S. Pavone is full professor at the University of Florence in the Department of Physics and at the European Laboratory for Non-Linear Spectroscopy (LENS), and group leader at the Biophotonics Laboratories. He obtained a PhD in optics in 1993 and spent two years as postdoctoral fellow at the Ecole Normale Superieure with the group of Claude Cohen Tannoudjy (Nobel Prize, 1997). His research group is involved in
developing new microscopy techniques for high-resolution and high-sensitivity imaging, and laser manipulation purposes. These techniques have been applied in singlemolecule biophysics, single-cell imaging, and optical manipulation. He is also engaged in tissue imaging research, for which nonlinear optical techniques have been applied to skin and neural tissue imaging. He is the author of more than 100 peer-reviewed journal articles, has delivered more than 60 invited talks. He coordinates various European projects and has organized international congresses. He is director of the international PhD program at LENS. He is on the editorial board of the journal Neurophotonics and is a principal investigator for the Human Brain Project, an EU Flagship initiative.
Shy Shoham is an Associate Professor at the Department of Biomedical Engineering in the Technion - Israel Institute of Technology. He was born in Rehovot, Israel, and holds a B.Sc. degree in Physics from Tel Aviv University and a Ph.D. in Bioengineering from the University of Utah. After completing his Ph.D., he was a Lewis-Thomas postdoctoral fellow at the department of Molecular Biology, Princeton University. In 2005 he joined the Technion's faculty of Biomedical Engineering, where he established the Neural Interface Engineering laboratory. His lab focuses on the development of implant-less retinal prostheses aimed at restoring vision loss from outer-retinal degenerative diseases, and on developing advanced technologies for acoustic neuromodulation, microscopic neuro-imaging and for bioengineering brain-like tissues. He is a recipient of a starting grant from the European Research Council, and of the Daniel Shiran and Juludan awards for engineering advances in bio-medicine, and is a member of the editorial boards of Journal of Neural Engineering, and of Translational Vision Science & Technology.