1st Edition

Handbook of Neurophotonics

Edited By Francesco S. Pavone, Shy Shoham Copyright 2020
    580 Pages 30 Color & 118 B/W Illustrations
    by CRC Press

    578 Pages 30 Color & 118 B/W Illustrations
    by CRC Press

    The Handbook of Neurophotonics provides a dedicated overview of neurophotonics, covering the use of advanced optical technologies to record, stimulate, and control the activity of the brain, yielding new insight and advantages over conventional tools due to the adaptability and non-invasive nature of light.

    Including 30 colour figures, this book addresses functional studies of neurovascular signaling, metabolism, electrical excitation, and hemodynamics, as well as clinical applications for imaging and manipulating brain structure and function. The unifying theme throughout is not only to highlight the technology, but to show how these novel methods are becoming critical to breakthroughs that will lead to advances in our ability to manage and treat human diseases of the brain.

    Key Features:

    • Provides the first dedicated book on state-of-the-art optical techniques for sensing and imaging across at the cellular, molecular, network, and whole brain levels.
    • Highlights how the methods are used for measurement, control, and tracking of molecular events in live neuronal cells, both in basic research and clinical practice.
    • Covers the entire spectrum of approaches, from optogenetics to functional methods, photostimulation, optical dissection, multiscale imaging, microscopy, and structural imaging.
    • Includes chapters that show use of voltage-sensitive dye imaging, hemodynamic imaging, multiphoton imaging, temporal multiplexing, multiplane microscopy, optoacoustic imaging, near-infrared spectroscopy, and miniature neuroimaging devices to track cortical brain activity.


    Preface, ix

    Contributors, xi

    Part I Function and Structural Neurophotonic Imaging

    Chapter 1 ¿ Miniaturized Optical Neuroimaging Systems 3

    Hang Yu, Janaka Senarathna, Betty M. Tyler, Nitis h V. Thakor, and Arvi nd P. Pathak

    Chapter 2 ¿ Functional Imaging with Light-Sheet Microscopy 21

    Raghav K. Chhetri and Philipp J. Keller

    Chapter 3 ¿ Two-Photon Microscopy in the Mammalian Brain 55

    Hod Dana and Shy Shoham

    Chapter 4 ¿ Light Field Microscopy for In Vivo Ca2+ Imaging 81

    Tobi as Nöbauer and Alip asha Vaziri

    Chapter 5 ¿ Genetically Encoded Activity Indicators 113

    Chenchen Song and Thomas Knöpfel

    Chapter 6 ¿ Functional Optoacoustic Imaging 129

    Daniel Razansky

    Chapter 7 ¿ Imaging Deep in the Brain with Wavefront Engineering 147

    Roarke Horstmeyer, Maximi llian Hoffmann, Haowen Ruan, Benjami n Judkewitz,

    and Changhuei Yang

    Chapter 8 ¿ Nanoscopic Imaging to Understand Synaptic Function 173

    Daniel Choquet and Anne-Sophie Hafner

    Chapter 9 ¿ Chemical Clearing of Brains 191

    Klaus Becker, Chris tian Hahn, Nina Jährling, Marko Pende,

    Inna Sabdyusheva-Litsc hauer, Saiedeh Saghafi, Martina Wanis , and Hans-Ulric h Dodt

    Chapter 10 ¿ Advanced Light-Sheet Microscopy to Explore Brain

    Structure on an Organ-Wide Scale 221

    Ludovic o Silvestri and Francesc o S. Pavone

    Part II Neurophotonic Control and Perturbation

    Chapter 11 ¿ Optogenetic Modulation of Neural Circuits 235

    Mathias Mahn, Oded Klavi r, and Ofer Yizhar

    Chapter 12 ¿ Molecular Photoswitches for Synthetic Optogenetics 271

    Shai Berlin and Ehud Y. Isacoff

    Chapter 13 ¿ Applications of Nanoparticles for Optical Modulation of

    Neuronal Behavior 293

    Chiara Pavi olo, Shaun Gietman, Daniela Duc, Sim on E. Moulton, and

    Paul R. Stoddart

    Chapter 14 ¿ Optical Stimulation of Neural Circuits in Freely Moving

    Animals 317

    Leore R. Heim and Eran Stark

    Chapter 15 ¿ Holographic Optical Neural Interfaces (HONIs) 333

    Shani Rosen, Shir Paluch, and Shy Shoham

    Chapter 16 ¿ Multi-Photon Nanosurgery 353

    Anna Letizia Allegra Masc aro and Francesc o Saverio Pavone

    Part III Clinical and Human Neurophotonics

    Chapter 17 ¿ High Resolution Diffuse Optical Tomography of the

    Human Brain 369

    Muriah D. Wheelock and Adam T. Eggebrecht

    Chapter 18 ¿ Human Brain Imaging by Optical Coherence Tomography 399

    Caroline Magnain, Jean C. Augustinack, Davi d Boas, Bruce Fisc hl, Taner Akkin,

    Ender Konukoglu, and Hui Wang

    Chapter 19 ¿ Acousto-Optic Cerebral Monitoring 439

    Mic hal Balberg and Revi tal Pery-Shechter

    Chapter 20 ¿ Neurophotonic Vision Restoration 459

    Adi Schejter Bar-Noam and Shy Shoham

    Chapter 21 ¿ Optical Cochlear Implants 473

    C.-P. Ric hter, Y. Xu, X. Tan, N. Xia, and N. Suematsu

    Chapter 22 ¿ Label-Free Fluorescence Interrogation of Brain Tumors 503

    Brad A. Hartl, Shami ra Sridharan, and Laura Marcu

    Chapter 23 ¿ Higher Harmonic Generation Imaging for Neuropathology 527

    Nikolay Kuzmi n, Sander Idema, Eleonora Aronic a, Philip C. de Witt Hamer,

    Pieter Wess eling, and Marie Louis e Groot

    INDEX, 545


    Francesco S. Pavone is full professor at the University of Florence in the Department of Physics and at the European Laboratory for Non-Linear Spectroscopy (LENS), and group leader at the Biophotonics Laboratories. He obtained a PhD in optics in 1993 and spent two years as postdoctoral fellow at the Ecole Normale Superieure with the group of Claude Cohen Tannoudjy (Nobel Prize, 1997). His research group is involved in
    developing new microscopy techniques for high-resolution and high-sensitivity imaging, and laser manipulation purposes. These techniques have been applied in singlemolecule biophysics, single-cell imaging, and optical manipulation. He is also engaged in tissue imaging research, for which nonlinear optical techniques have been applied to skin and neural tissue imaging. He is the author of more than 100 peer-reviewed journal articles, has delivered more than 60 invited talks. He coordinates various European projects and has organized international congresses. He is director of the international PhD program at LENS. He is on the editorial board of the journal Neurophotonics and is a principal investigator for the Human Brain Project, an EU Flagship initiative.

    Shy Shoham is an Associate Professor at the Department of Biomedical Engineering in the Technion - Israel Institute of Technology. He was born in Rehovot, Israel, and holds a B.Sc. degree in Physics from Tel Aviv University and a Ph.D. in Bioengineering from the University of Utah. After completing his Ph.D., he was a Lewis-Thomas postdoctoral fellow at the department of Molecular Biology, Princeton University. In 2005 he joined the Technion's faculty of Biomedical Engineering, where he established the Neural Interface Engineering laboratory. His lab focuses on the development of implant-less retinal prostheses aimed at restoring vision loss from outer-retinal degenerative diseases, and on developing advanced technologies for acoustic neuromodulation, microscopic neuro-imaging and for bioengineering brain-like tissues. He is a recipient of a starting grant from the European Research Council, and of the Daniel Shiran and Juludan awards for engineering advances in bio-medicine, and is a member of the editorial boards of Journal of Neural Engineering, and of Translational Vision Science & Technology.