A comprehensive and groundbreaking collection of ideas for plant improvement
Most of the world’s supply of legumes is cultivated under adverse conditions that make this commercially important crop susceptible to the vagaries of nature and damaging stresses. Genetic manipulation has become a proven way for cultivators to battle these problems; but there has been a lack of reliable information on the practical aspects of this promising method. Handbook of New Technologies for Genetic Improvement of Legumes fills this void by providing a vast array of groundbreaking essays from some of the discipline’s most renowned experts. This unique collection is your single source for not only the most up-to-date accounts of genetic manipulation employed today, but also ideas for realistic and practical uses.
Comprehensive in its approach, Handbook of New Technologies for Genetic Improvement of Legumes presents the latest research on all aspects of genetically modified legume production. With contributions from over 90 international experts, this eye-opening resource offers an in-depth exploration of key topics such as, genetic transformation technology, suppression-subtractive hybridization, c-DNA micro arrays, molecular mapping, tagging regulatory elements, and real-time PCR. Additionally, this text features a wealth of detailed references and informative tables to clearly explain pertinent data.
Chapters in Handbook of New Technologies for Genetic Improvement of Legumes detail both family- and species-specific topics such as: • agrobacterium-mediated transformation in trifolium and medicago • biotechnological approaches to soybean improvement • transgenic approaches for enhancement of vitamin E • particle bombardment and SAAT approaches • agrobacterium tumefaciens-mediated transformation and in vitro regeneration • plant regeneration via organogenesis, somatic embryogenesis, and protoplast culture • somaclonal variation and its exploitation • cotyledonary node and embryo axes as explants • in planta transformation of pisum sativum l. • de novo regeneration protocol • reverse genetic approaches in medicago truncatula • tagging regulatory elements in plants • gene expression analysis with DNA array technology • advent of diverse DNA markers to decipher genome sequence polymorphism • defensins in legumes • legume-nematode interaction with reference to model legumes • and much, much more! Handbook of New Technologies for Genetic Improvement of Legumes is an invaluable resource for researchers and educators in the area of plant genetic manipulation and plant biotechnology as well as scientists, educators, and graduate students focusing on the genetic manipulation of legumes.
Table of Contents
Genetic Engineering for Forage Quality in Alfalfa, C. Sengupta-Gopalan. Genetic Transformation in Trifolium and Medicago. Agrobacterium-Mediated Genetic Transformation of Alfalfa. Agrobacterium-Mediated Transformation of Medicago truncatula. Biotechnological Approaches to Soybean Improvement. Agrobacterium-Mediated Genetic Transformation of Soybean. Genetic Transformation of Soybean Using Particle Bombardment and SAAT Approaches. Genetic Transformation as a Tool for Improvement of Pigeon Pea. Genetic Transformation of Chickpea Using Cotyledonary Node Explants. Genetic Transformation of Phaseolus and Vigna Species. Genetic Engineering in Azuki Bean. Somaclonal Variation and Genetic Transformation in Lathyrus sativus. Agrobacterium; Mediated Transformation of Pea. Genetic Transformation of Pea by Microprojectile Bombardment. In Planta Transformation of Pisum sativum L. Using Microinjection and Electroporation. Agrobacterium-Mediated Genetic Transformation of Peanut. Cotyledonary Node and Embryo Axes as Explants in Legume Transformation with Special Reference to Peanut. A Novel in Planta Approach of Gene Transfer for Legumes. Agrobacterium-Mediated Genetic Transformation of Faba Bean. Genetic Transformation of Lotus Species. Genetic Transformation of Tree Legume Leucaena leucocephala. Genetic Transformation of Tree Legume Robinia. Medicago truncatula as A Model System in Plant Molecular Biology. Reverse Genetic Approaches in Medicago truncatula. Fluorescence in Situ Hybridization on Medicago truncatula Chromosomes. Molecular and Genetic Analyses of Transgenic Plants. Tagging Regulatory Elements in Plants. cDNA-AFLP Transcriptome Analysis in Legumes. Suppression Subtractive Hybridization to Identify Novel Genes in Legumes. Quantitative PCR in the Analysis of Transgenic Plants. Gene Expression Analysis with DNA Array Technology in Legumes. Advent of Diverse DNA Markers to Decipher Genome Sequence Polymorphism. Applicability of DNA Markers for Genome Diagnostics of Grain Legumes. Genetic Maps and Molecular Markers in The Genus Medicago. Management of Abiotic Stresses in Grain Legumes through Manipulation of Genes for Compatible Solutes. Legume-Nematode Interaction with Reference to Model legumes. Defensins in Legumes.