1st Edition

Handbook of Nucleic Acid Purification





ISBN 9781138113879
Published June 16, 2017 by CRC Press
584 Pages - 53 B/W Illustrations

USD $86.95

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Book Description

An Indispensable Roadmap for Nucleic Acid Preparation

Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic acids, and these have become a cornerstone for all branches of modern biological sciences.

The Handbook of Nucleic Acid Purification provides researchers and students with an all-encompassing volume on nucleic acid extraction strategies. Due to the complexities within prokaryotic and eukaryotic cells, purification of the nucleic acids often forms a vital first step in the study of molecular biology of living organisms as well as in the evolutionary/phylogenetic analysis of ancient specimens.

Bringing together contributions from leading researchers, the handbook presents a comprehensive catalog of nucleic acid isolation methods. It includes dedicated sections on strategies for viruses, bacteria, fungi, parasites, insects, mammals, and plants, as well as for ancient samples, with an additional emphasis on sample preparation methods for direct molecular applications.

Each chapter in this handbook:

  • Explores the biological background important to understanding specific organisms and specimens
  • Reviews principles and current techniques for efficient isolation
  • Discusses challenges and future trends relating to improved recovery of nucleic acids

Besides providing an updated, reliable reference for anyone with an interest in molecular biology, this book offers a practical guide for clinical, forensic, and research scientists involved in molecular analysis of biological specimens. It also constitutes a convenient resource for students in other areas of biological sciences, and an indispensable roadmap for both new and experienced researchers wishing to acquire or sharpen their skills in nucleic acid preparation.

Table of Contents

Nucleic Acids: Structures, Functions, and Applications, Valentin V. Vlassov, Dmitrii V. Pyshnyi, and Pavel E. Vorobjev

Purification of Nucleic Acids from Viruses
Isolation of Viral DNA from Cultures, Larry A. Hanson
Isolation of Viral RNA from Cultures, Ling Zhao and Zhen F. Fu
Preparation of Viral Samples for Direct Molecular Applications, Grant S. Hansman

Purification of Nucleic Acids from Bacteria
Chapter 5 Isolation of Bacterial DNA from Cultures, Dongyou Liu
Isolation of Bacterial RNA from Cultures, Som Subhra Chatterjee and Trinad Chakraborty
Isolation of Plasmids, Armelle Bigot and Alain Charbit
Preparation of Bacterial Samples for Direct Molecular Applications, Knut Rudi, Robert C. Wilson, Kjetill S. Jakobsen, and Dongyou Liu

Purification of Nucleic Acids from Fungi
Isolation of Nucleic Acids from Yeasts, Lakshman P. Samaranayake, Nipuna B. Parahitiyawa, and C. Jayampath Seneviratne
Isolation of Nucleic Acids from Filamentous Fungi, Margaret E. Katz and Brian F. Cheetham
Preparation of Fungal Specimens for Direct Molecular Applications, Françoise Irlinger, Nicolas Berthet, Tatiana Vallaeys, Valérie Vasseur, Renaud Ioos, Marc Buée, and Jérôme Mounier

Purification of Nucleic Acids from Parasites and Insects
olation of Nucleic Acids from Protozoa, Vitaliano A. Cama and Lihua Xiao
Isolation of Nucleic Acids from Helminthes, Munehiro Okamoto and Akira Ito
Isolation of Nucleic Acids from Insects, Kun Yan Zhu
Preparation of Parasitic Specimens for Direct Molecular Applications, Huw Vaughan Smith and Rosely Angela Bergamin Nichols

Purification of Nucleic Acids from Mammals
Isolation of Nucleic Acids from Body Fluids, Karen Page and Jacqueline Amanda Shaw
Isolation of Nucleic Acids from Soft Tissues, Sergey Kovalenko
Isolation of Nucleic Acids from Hard Tissues. Judith Weidenhofer and Jennifer A. Byrne
Isolation of DNA from Ancient Samples, Paula F. Campos, Eske Willerslev, and M. Thomas P. Gilbert
Preparation of Forensic Samples for Direct Molecular Applications, Erin K. Hanson and Jack Ballantyne

Purification of Nucleic Acids from Plants
Isolation of Nucleic Acids from Plants, Song Weining and Dongyou Liu
Isolation of Megabase-Sized DNA Fragments from Plants, Meiping Zhang, Yaning Li, and Hong-Bin Zhang

Purification of Nucleic Acids from Miscellaneous Sources
Purification of Nucleic Acids from Gels, Chunfang Zhang and Theo Papakonstantinou
Index

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Reviews

"I was very impressed with the amount of technical detail in the book, having comprehensive descriptions of in-house and commercial methods, together with consideration of the theoretical basis of methods. There is so much more to this book than merely being a laboratory manual. The chapters are very well written, reference lists are comprehensive, and figures and diagrams are good."

– Vince Mulholland, SASA, in Microbiology Today, May 2009