Any scientist or technician involved in pre-clinical drug studies knows that infusing animal models with test substances is no simple matter, especially if one wants to do it humanely as well as guarantee results that are not compromised by the procedure. The challenge becomes compounded if one is employing a variety of species. What works for a rat or mouse does not necessarily work for a rabbit or primate.
The Handbook of Pre-clinical Continuous Intravenous Infusion answers the needs of researchers and technicians by providing complete instructions for a wide range of infusion techniques delivered to several species. With sections devoted to rats, mice, rabbits, dogs, mini-pigs, and nonhuman primates (both large and small), this thorough text gives step-by-step instructions for the use of a variety of techniques, and equipment. It includes procedures for multidose infusion toxicity and reproductive toxicology studies, and provides guidance on the use of a variety of equipment, including tail cuffs, harnesses, and implanted buttons. It also offers detailed information on surgical preparation for primates and mini-pigs, along with advice about the use of preoperative anesthesia, post surgical analgesics, and antibiotic administration, as well as valuable input on surgical suite design.
"… I was amazed and gratified about the level of detail and the practical emphasis on technical expertise and animal well-being. This book is unique in having that as an overt aim."
From the Foreword, David B. Morton, University of Birmingham, UK
The text concludes with a chapter on general non-species-specific information that discusses common pathological findings in continuous infusion studies, the use of mini-osmatic pumps, and controllable variables such as vehicles, rates of administration and volumes. With contributions from several established experts in the handling of pre-clinical animal models, this authoritative volume removes the need for any guesswork, allowing technicians and researchers to proceed with confidence in undertaking critical studies.
Table of Contents
Introduction; D. Smith and G. Healing
CONTINUOUS INTRAVENOUS INFUSION IN THE RAT
Femoral Cannulation Using the Jacket/Harness Model in the Rat; O. Green and D. Patten
Femoral Cannulation Using the Tail Cuff Exteriorisation Method in the Rat; A. McCarthy
Tethering and the Implanted Button Model in the Rat; E. Miedel
Multidose Infusion Toxicity Studies in the Rat G. Healing
Reproductive Toxicology Studies by Infusion Studies in the Rat P. Barrow
CONTINUOUS INTRAVENOUS INFUSION IN THE MOUSE
Femoral Cannulation Using the Tail Cuff Model in the Mouse H. Van Wijk and D. Robb
Multidose Infusion Toxicity Studies in the Mouse H. Van Wijk and D. Robb
Jugular Cannulation and Efficacy Studies in the Mouse I. Horii
CONTINUOUS INTRAVENOUS INFUSION IN THE RABBIT
Femoral Cannulation of the Rabbit for Reproductive Toxicity Studies C. Copeman and K. Robinson
Jugular Cannulation of the Rabbit for Reproductive Toxicity Studies E. Gordon and S. Lynch
CONTINUOUS INTRAVENOUS INFUSION IN THE DOG
Jugular Cannulation of the Dog N. Pickersgill
The Non-Ambulatory Model in Dog Multidose Infusion Toxicity Studies N. Pickersgill
The Ambulatory Model in Dog Multidose Infusion Toxicity Studies T.R. Gleason and C. Chengelis
CONTINUOUS INTRAVENOUS INFUSION IN THE PRIMATE
Surgical Preparation of the Large Primate V. Mendenhall, S. Cornell and M. Scalaro
Multidose Infusion Toxicity Studies in the Large Primate M. Walker
Surgical Preparation and Multidose Infusion Toxicity Studies in the Marmoset C. Schnell
CONTINUOUS INTRAVENOUS INFUSION IN THE MINI-PIG
Surgical Preparation of the Mini-Pig P. Brinck
Multidose Infusion Toxicity Studies in the Mini-Pig P. Brinck
Reproductive Toxicity Studies in the Mini-Pig P. McAnulty
CONTINUOUS INTRAVENOUS INFUSION - GENERAL
Common Pathological Findings in Continuous Infusion Studies J. Evans and P. Kerry
The Use of Mini-Osmotic Pumps in Continuous Infusion Studies L. Perkins, C. Peer and P. Murphy-Hackley
The Contribution of Vehicles, Rates of Administration and Volumes to Infusion Studies K. Hickling and D. Smith
Equipment for Continuous Intravenous Infusion A. Jacobson
"…an excellent buy for anyone involved in drug development, risk assessment or within drug licensing agencies and in academic research. …This book represents a benchmark achievement for this technique …it will become the standard for many years to come"
British Toxicology Society Newsletter