1st Edition

Handbook of Tissue Optical Clearing
New Prospects in Optical Imaging

  • Available for pre-order. Item will ship after November 18, 2021
ISBN 9780367895099
November 18, 2021 Forthcoming by CRC Press
600 Pages 577 B/W Illustrations

USD $300.00

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Book Description

Biomedical photonics is currently one of the fastest growing fields, connecting research in physics, optics, and electrical engineering coupled with medical and biological applications. It allows for the structural and functional analysis of tissues and cells with resolution and contrast unattainable by any other methods.

However, the major challenges of many biophotonics techniques are associated with the need to enhance imaging resolution even further to the sub-cellular level as well as translate them for in vivo studies. The tissue optical clearing method uses immersion of tissues into optical clearing agents (OCAs) that reduces the scattering of tissue and makes tissue more transparent and this method has been successfully used ever since.

This book is a self-contained introduction to tissue optical clearing, including the basic principles and in vitro biological applications, from in vitro to in vivo tissue optical clearing methods, and combination of tissue optical clearing and various optical imaging for diagnosis. The chapters cover a wide range of issues related to the field of tissue optical clearing: mechanisms of tissue optical clearing in vitro and in vivo; traditional and innovative optical clearing agents; recent achievements in optical clearing of different tissues (including pathological tissues) and blood for optical imaging diagnosis and therapy.

This book provides a comprehensive account of the latest research and possibilities of utilising optical clearing as an instrument for improving the diagnostic effectiveness of modern optical diagnostic methods.

The book is addressed to biophysicist researchers, graduate students and postdocs of biomedical specialties, as well as biomedical engineers and physicians interested in the development and application of optical methods in medicine.


Key features:

  • The first collective reference to collate all known knowledge on this topic
  • Edited by experts in the field with chapter contributions from subject area specialists
  • Brings together the two main approaches in immersion optical clearing into one cohesive book

Table of Contents

Part I Basic principles of tissue optical clearing

Chapter 1. Tissue optical clearing mechanisms

Tingting Yu, Dan Zhu, Luís Oliveira, Elina A. Genina, Alexey N. Bashkatov, Valery V. Tuchin

Chapter 2.  Tissue optical clearing for Mueller matrix microscopy

Nan Zeng, Honghui He, Valery V. Tuchin, and Hui Ma

Chapter 3.  Traditional and innovative optical clearing agents

Elina Genina, Vadim D. Genin, Jingtan Zhu, Alexey Bashkatov, Dan Zhu, Valery Tuchin

Chapter 4.  Chemical enhancers for improving tissue optical clearing efficacy

Dan Zhu, Yanmei Liang, Xingde Li, and Valery Tuchin

Chapter 5.  Human skin autofluorescence and optical clearing

Walter Blondel, Marine Amouroux, Sergey Zaytsev, Elina Genina, Victor Colas, Christian Daul, Alexander Pravdin and Valery Tuchin

Chapter 6.  Molecular modeling of post-diffusion phase of optical clearing of biological tissues

K.V. Berezin, K. N. Dvoretskiy, M. L. Chernavina, M.A. Likhter, and V.V. Tuchin

Chapter 7. Refractive index measurements of tissue and blood components and OCAs in a wide spectral range

Ekaterina N. Lazareva, Luís Oliveira, Irina Yu. Yanina, Nikita V. Chernomyrdin, Guzel R. Musina, Daria K. Tuchina, Alexey N. Bashkatov, Kirill I. Zaytsev, and Valery V. Tuchin

Chapter 8. Water migration at skin optical clearing

Anton Yu. Sdobnov, Johannes Schleusener, Jürgen Lademann, Valery V. Tuchin, Maxim E. Darvin

Chapter 9.  Optical and mechanical properties of the cartilage during optical clearing

Yulia Alexandrovskaya, Olga Baum, Vladimir Zaitsev, Alexander Sovetsky, Alexander Matveyev, Lev Matveev, Kirill Larin, Emil Sobol, Valery Tuchin

Chapter 10.  Compression optical clearing

Olga Zurukina, Yury P. Sinichkin

Part II Tissue optical clearing method for biology (3D imaging)

Chapter 11. Optical clearing for multiscale tissues and the quantitative evaluation of clearing methods in mouse organs

Tingting Yu, Jianyi Xu, Dan Zhu

Chapter 12.  Ultrafast aqueous clearing methods for 3D imaging

Tingting Yu, Jingtan Zhu, Dan Zhu

Chapter 13. The Challenges and Opportunities in Hydrophilic Tissue Сlearing Methods

Etsuo A. Susaki, Hiroki R. Ueda 

Chapter 14. Combination of tissue optical clearing and 3D fluorescence microscopy for high-throughput imaging of entire organs and organisms

Peng Fei and Chunyu Fang

Chapter 15.  Endogenous fluorescence preservation from solvent-based optical clearing

Tingting Yu, Yisong Qi, Dan Zhu

Chapter 16.  Progress in ex situ tissue optical clearing – shifting immuno-oncology to the third dimension

Paweł Matryba, Leszek Kaczmarek, Jakub Gołąb

Part III Towards in vivo tissue optical clearing

Chapter 17.  In vivo skin optical clearing methods for blood flow and cell imaging

Dongyu Li, Wei Feng, Rui Shi, Dan Zhu

Chapter 18.  In vivo skull optical clearing for imaging cortical neuron and vascular structure and function

Dongyu Li, Yanjie Zhao, Chao Zhang, Dan Zhu

Chapter 19.  In vivo skin optical clearing in humans

Elina Genina, Alexey N. Bashkatov, Valery Tuchin, Vladimir Zharov

Chapter 20.  Optical clearing of blood and blood components

Olga Zhernovaya, Elina A. Genina, Valery V. Tuchin, Alexey N. Bashkatov

Chapter 21.  Blood and lymph flow imaging at optical clearing

Polina A. Dyachenko (Timoshina), Arkady C. Abdurashitov, Oxana V. Glushkovskaya, and Valery V. Tuchin

Part IV Combination of tissue optical clearing and optical imaging/spectroscopy for diagnostics

Chapter 22.  Optical clearing aided photoacoustic imaging in vivo

Yong Zhou and Lihong V. Wang

Chapter 23. Enhancement of contrast in photoacoustic – fluorescence tomography and cytometry using optical clearing and contrast agents

Julijana Cvjetinovic, Daniil Nozdriukhin, Maksim Mokrousov, Alexander Novikov, Marina Novoselova, Valery Tuchin, Dmitry Gorin

Chapter 24.  Tissue optical clearing in the terahertz range

Olga Smolyanskaya, Kirill Zaytsev, Irina Dolganova, Guzel Musina, Daria Tuchina, Maxim Nazarov, Alexander Shkurinov, Valery Tuchin

Chapter 25.  Magnetic resonance imaging study of diamagnetic and paramagnetic agents for optical clearing of tumor-specific fluorescent signal in vivo

Alexei A. Bogdanov Jr., Natalia I. Kazachkina, Victoria V. Zherdeva, Irina G. Meerovich, Daria K. Tuchina, Ilya D. Solovyev, Alexander P. Savitsky, Valery V. Tuchin

Chapter 26.  Use of an optical clearing and index matching agents to enhance the imaging of caries lesions and internal structures in teeth using optical coherence tomography and SWIR imaging

Daniel Fried

Chapter 27.  Optical clearing of adipose tissue

Irina Yu. Yanina, Yohei Tanikawa, Daria K. Tuchina, Polina A. Dyachenko (Timoshina), Yasunobu Iga, Shinichi Takimoto, Elina A. Genina, Alexey N. Bashkatov, Georgy S. Terentyuk, Nikita A. Navolokin, Alla B. Bucharskaya, Galina N. Maslyakova, Valery V. Tuchin

Chapter 28.  Diabetes mellitus-induced alterations of tissue optical properties, optical clearing efficiency and molecular diffusivity

Daria Tuchina, Valery Tuchin

Chapter 29.  Tissue optical clearing for in vivo detection and imaging diabetes induced changes in cells, vascular structure and function

Dongyu Li, Wei Feng, Rui Shi, Valery Tuchin, Dan Zhu

Chapter 30. Light operation on cortex through optical clearing skull window

Dongyu Li, Chao Zhang, Oxana Glushkovskaya, Yanjie Zhao, Dan Zhu

Chapter 31.  The role of optical clearing to enhance the applications of in vivo OCT and photodynamic therapy: towards PDT of pigmented melanomas and beyond?

Layla Pires, Michelle Barreto Requena, Valentin Demidov, Ana Gabriela Salvio, I. Alex Vitkin, Brian C. Wilson, Cristina Kurachi

Chapter 32.  Combination of tissue Optical clearing and OCT for tumor diagnosis via permeability coefficient measurements

Qingliang Zhao

Chapter 33.  Optical clearing for cancer diagnostics and monitoring

Luis Oliveiro, Valery Tuchin

Chapter 34. Contrast enhancement and tissue differentiation in optical coherence tomography with mechanical compression

Pavel Agrba and Mikhail Kirillin

Chapter 35.  Measurement of the dermal beta-carotene in the context of the multimodal optical clearing

M. A. Ansari, V.V. Tuchin

Chapter 36.  Optical clearing and molecular diffusivity of hard and soft oral tissues

Alexey A. Selifonov and Valery V. Tuchin

Chapter 37. Optical clearing and Raman spectroscopy: in vivo applications

Qingyu Lin, Ekaterina N. Lazareva, Vyacheslav I. Kochubey, Yixiang Duan, and Valery V. Tuchin

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Valery V. Tuchin - Corresponding Member of the Russian Academy of Sciences, Professor, Head of the Department of Optics and Biophotonics and Director of the Scientific Medical Center of the Saratov State University. He is also the Head of the laboratory for laser diagnostics of technical and living systems at the Institute of Precise Mechanics and Control of the RAS, the supervisor of the Interdisciplinary Laboratory of Biophotonics at the National Research Tomsk State University and the Femtomedicine Laboratory of the ITMO University.

His research interests include biophotonics, biomedical optics, tissue optics, laser medicine, tissue optical clearing, and nanobiophotonics.

He is a member of SPIE, OSA and IEEE, Visiting Professor at HUST (Wuhan) and Tianjin Universities in China, and Adjunct Professor at the University of Limerick (Ireland) and the National University of Ireland (Galway).

Professor Tuchin was elected Fellow SPIE and OSA, he was awarded many titles and awards, including Honored Scientist of the Russian Federation, Honored Professor of SSU, Honored Professor of Finland (FiDiPro), SPIE in the field of optical education, Chime Bell of Hubei province (China), Joseph Goodman (OSA / SPIE) for Outstanding Monograph (2015), Michael Feld (OSA) for Pioneering Research in Biophotonics (2019), the Medal of the D.S. Rozhdestvensky Optical Society (2018) and the Alexander Mikhailovich Prokhorov medal of the Academy of Engineering Sciences named after A.M. Prokhorov (2021).

He is the author of over 700 articles (Web of Science), 30 monographs and textbooks, has over 60 patents, his work has been cited over 30,000 times.

Dan Zhu is a distinguished professor of Huazhong University of Science & Technology (HUST), and the Vice-Director of Wuhan National Optoelectronics Laboratory,Vice-Director of Key Laboratory of Biomedical Photonics (HUST), Ministry of Education.

Her research interests include tissue optical imaging theory and methods, especially, focus on tissue optical clearing imaging and applications. She has authored more than 150 papers including Science Advances, Nature Communications, et al.

She was elected Fellow of SPIE, and Secretary General & Vice President of Biomedical Photonics Committee of Chinese Optical Society. She serves for some international or domestic conference as chair or co-chair, and some journals as guest editor or editorial member, including Biomedical Optics Express, Journal of Biomedical Optical, Scientific Reports, Journal of Innovative Optical Health Sciences, Frontier of Optoelectronics et al.

Elina A. Genina, PhD, Dr. Sc. is a professor in the Departments of Optics and Biophotonics at Saratov State University. Her research interests include biomedical optics, laser medicine, nanobiophotonics, and development of methods for control of tissue optical properties. She is a coauthor of more than 300 peer-reviewed publications, analytical reviews, book chapters, and patents on Biomedical Optics, guest editor of 18 special issues of journals and proceedings. She is a member of editorial board of J. Innovative Optical Health Sciences, Diagnostics, J. Biomedical Photonics & Engineering, and The Open Biomedical Engineering Journal. She is a scientific secretary of International Symposium on Optics and Biophotonics (Saratov Fall Meeting) and co-chair of a conference in the framework of the Symposium. She has more than 4000 citations and h-index 30.