This comprehensive book is a compilation of Professor Lubomir S. Hnilica's twenty years of research experimentally addressing the chemistry and the biological functions of chromosomal proteins. The histones and other nuclear proteins found associated with DNA in a number of tissues and cell types are featured. Lubomir Hnilica played a major role in establishing the extent to which these basic chromosomal polypeptides are conserved and the manner in which they interact with DNA to modify chromatin structure. In addition, non-histone chromosomal protein research is explained, and his technique of applying several biochemical and immunological approaches to the characterization of this complex and heterogeneous class of chromosomal polypeptides is discussed. Highlighted is the use of chemical crosslinking for studying protein/DNA interactions in intact cells. The proteins as well as the structure, organization, and regulation of the genes are also presented.
"…a readable and highly commendable volume…to all cell biologists interested in chromatic structure and function."
-Cell Biochemistry and Function
THE PROTEINS. Human Histone Variants. Histone Occurrence, Isolation, Characterization, and Biosynthesis. Specificity and Distribution of Sperm Basic Proteins. Histone and Basic Nuclear Protein Transitions in Mammalian Spermatogenesis. A Basic Nonhistone Nuclear Protein in Human Myeloid Cells. Posttranslational Modifications of Histones. STRUCTURE, ORGANIZATION, AND REGULATION OF THE GENES. Organization of Histone Genes and Their Variants. Cell Cycle Dependent Human Histone Genes: Their Organization and Regulation. Histone Gene Expression Not Coupled to DNA Synthesis. Structures of Testis-Specific Histones, Spermatid Transition Proteins, and Their Genes in Mammals. Mammalian Protamines and Their Expression. HMG Genes and Their Expression